本文關鍵詞： 帕金森病 深部腦磁刺激 運動行為學 不良反應 安全性　出處：《首都醫(yī)科大學學報》2017年02期 　論文類型：期刊論文

【摘要】：目的評估深部腦磁刺激(deep-brain magnetic stimulation,DMS)對單側帕金森病(Parkinson's disease,PD)模型大鼠運動障礙的治療作用及DMS治療的安全性。方法立體定位注射六羥基多巴胺(6-hydroxydopamine,6-OHDA)于大鼠右側紋狀體制備單側帕金森病模型,假手術對照組(Sham組)依據(jù)同樣方法注射0.9%(質量分數(shù))氯化鈉注射液。利用阿撲嗎啡篩選模型后,造模成功的動物采用均衡隨機化分組的方式分為模型對照組(Model組)、δ節(jié)律DMS治療組(DMS-δ組)、γ節(jié)律DMS治療組(DMS-γ組),其中兩種DMS治療組行每天40 min、持續(xù)4周的DMS治療。通過碰壁實驗檢測大鼠運動行為,通過大鼠體質量的測定、外周血淋巴細胞的分類及計數(shù)、主要組織器官的蘇木精-伊紅(Hematoxylin-Eosin staining,HE)染色評價DMS治療的安全性。結果 (1)檢測大鼠運動行為顯示,DMS-γ治療組損傷側的肢體利用率顯著高于Model組,差異具有統(tǒng)計學意義(P0.05),而DMS-δ組相較于Model組差異無統(tǒng)計學意義(P0.05)。(2)Sham組、Model組、DMS-δ組、DMS-γ組大鼠體質量差異均無統(tǒng)計學意義(P0.05)。(3)Sham組、Model組、DMS-δ組、DMS-γ組大鼠外周血總淋巴細胞與T細胞的數(shù)量和比例差異均無統(tǒng)計學意義(P0.05)。DMS-δ組大鼠的自然殺傷(natural killer,NK)細胞數(shù)量和比例相較于Model組有顯著改善。(4)HE染色顯示,DMS對大鼠主要器官包括心、肝、脾、肺、腎、胃、小腸、睪丸均未造成明顯的組織病理變化。結論 DMS-γ治療可改善PD大鼠雙側肢體不對稱性運動障礙,且DMS對PD大鼠運動行為的治療中無明顯不良反應,表明DMS在對PD大鼠運動癥狀的治療中具有很好的安全性。
[Abstract]:Objective to evaluate the therapeutic effect of deep-brain magnetic stimulation (magnetic) on motor disorders in rats with unilateral Parkinson's disease (PD) and the safety of DMS treatment. Methods A stereotactic injection of 6-hydroxydopamine 6-hydroxydopamine 6-OHDAin was made in the right striatum of rats. Lateral Parkinson's disease model, Sham-operated control group (Sham group) was injected with 0.9 sodium chloride injection (mass fraction) according to the same method. The model was screened with apomorphine. The successful animals were randomly divided into three groups: model group (model group), DMS group (未 rhythm), DMS- 緯 group (緯 rhythm DMS). Two DMS treatment groups were treated with DMS for 40 min per day for 4 weeks. The movement behavior of rats was detected by the experiment of bumping through the wall. By measuring the body mass of rats, the peripheral blood lymphocytes were classified and counted. The safety of DMS was evaluated by hematoxylin-eosin hematoxylin-eosin staining in main tissues and organs. Results 1) the limb utilization rate of injured side in DMS- 緯 treatment group was significantly higher than that in Model group. There was no significant difference between DMS- 未 group and Model group. There was no significant difference in body mass between DMS- 未 group and DMS- 未 group compared with Model group. There was no significant difference in body mass between DMS- 未 group and DMS- 緯 group. There was no significant difference in body mass between DMS- 未 group and DMS- 未 group compared with DMS- 未 group, DMS- 未 group, DMS- 緯 group, DMS- 未 group, DMS- 未 group, DMS- 緯 group, DMS- 未 group, DMS- 未 group, DMS- 未 group, DMS- 未 group and DMS- 緯 group. The number and proportion of natural killer killer NKK cells in DMS- 未 group were significantly improved compared with that in Model group. The results showed that the number and proportion of natural killer NK cells in DMS- 未 group were significantly better than those in Model group, which showed that the number and proportion of natural killer NK cells in DMS- 未 group were significantly better than those in Model group. Liver, spleen, lung, kidney, stomach, small intestine and testis did not cause obvious histopathological changes. Conclusion DMS- 緯 therapy can improve bilateral limb asymmetric motor disorder in PD rats, and there is no obvious adverse reaction in the treatment of PD rats with DMS. The results suggest that DMS is safe in the treatment of motor symptoms in PD rats.
【作者單位】： 首都醫(yī)科大學基礎醫(yī)學院神經生物學系;教育部神經變性病重點實驗室;北京腦重大疾病研究院;首都醫(yī)科大學基礎醫(yī)學院生理學與病理生理學系;
【基金】：國家重點基礎研究發(fā)展計劃(“973”計劃)(2011CB504100) 北京市自然科學基金項目(7082008) 北京市科學技術委員會專項資助(Z161100002616007) 北京市屬高等學校創(chuàng)新團隊建設與教師職業(yè)發(fā)展計劃項目(IDHT20140514)~~
【分類號】：R742.5;R-332

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