本文關(guān)鍵詞： 跑臺運(yùn)動 帕金森病 運(yùn)動皮層 局部場電位 β振蕩　出處：《西安體育學(xué)院學(xué)報(bào)》2017年05期 　論文類型：期刊論文

【摘要】：目的觀察運(yùn)動對帕金森病(parkinson's disease,PD)模型大鼠初級運(yùn)動皮層(M1)異常β振蕩的調(diào)節(jié)作用,探究運(yùn)動干預(yù)改善PD行為功能的可能原因。方法清潔級SD大鼠18只分為3組,即假手術(shù)組(Control,n=5)、PD組(PD,n=6)和PD運(yùn)動組(PD+Ex,n=7),于內(nèi)側(cè)前腦束注射6-羥基多巴(6-OHDA)同時(shí)于M1區(qū)植入多通道電極。大鼠在造模后第1周頸部皮下注射阿樸嗎啡(APO)旋轉(zhuǎn)行為實(shí)驗(yàn)評價(jià)模型成功;PD+Ex組在第1周開始進(jìn)行跑臺訓(xùn)練(11 m/min,30 min/d,5 d/周,4周),各組大鼠在第0/1/2/3/4采用自主錯步儀進(jìn)行行為測試;采用在體多通道電生理技術(shù),觀察各組大鼠M1區(qū)在第0/1/2/3/4的局部場電位(LFPs),免疫組化技術(shù)檢測黑質(zhì)和紋狀體內(nèi)酪氨酸羥化酶(TH)表達(dá)。結(jié)果 TH免疫組化結(jié)果顯示,PD+Ex組較PD組無顯著性改變(P0.05);自主錯步行為測試結(jié)果發(fā)現(xiàn),PD+Ex組的通過時(shí)間、前肢錯步數(shù)和尾部滑落數(shù)及潛伏期較PD組均有顯著性改善(P0.01)。電生理學(xué)結(jié)果顯示,PD組較Control組10~30 Hz的β頻段異常增高(P0.01);PD+Ex組在第1周β振蕩的PSD較第0周無顯著改變(P0.05),在第2/3/4周(P0.05)顯著降低。結(jié)論運(yùn)動干預(yù)調(diào)節(jié)運(yùn)動皮層的功能活性,抑制M1區(qū)異常β振蕩,進(jìn)而改善其步態(tài)及自主活動能力。推測運(yùn)動降低PD模型大鼠M1區(qū)異常β振蕩介導(dǎo)了皮層-紋狀體Glu能突觸可塑性,調(diào)控輸入至基底神經(jīng)節(jié)的運(yùn)動編碼信息,最終改善大鼠的行為功能。
[Abstract]:Objective to observe the effect of exercise on 尾 oscillation in primary motor cortex (M1) of Parkinson's disease (PD) rats, and to explore the possible causes of exercise intervention to improve PD behavioral function. Methods 18 clean SD rats were divided into 3 groups. That is, the sham operation group (control group) and PD group (PD group) and PD exercise group (PD exann 7) were injected into the medial forebrain tract of 6-hydroxydopazone 6-OHDA.The rats were injected with multichannel electrodes in M1 region simultaneously. The rotation behavior of apomorphine apo was injected subcutaneously into the neck of rats at the first week after modeling. The model was successfully established in the PD Ex group at the beginning of the first week. The rats in each group were tested with an autonomous stagger at 0 / 1 / 2 / 3 / 4 by using an autonomous error-walker. The rats in each group were trained at 11 m / min ~ 30 min / d ~ (-1) min / d ~ (-1) / d ~ (5) d / week for 4 days / week. Using in vivo multichannel electrophysiology, The expression of tyrosine hydroxylase (THH) in substantia nigra and striatum was detected by immunohistochemistry. The wrong walk was used to find the passage time of the PD Ex group as a result of the test. The results of electrophysiology showed that the PSD of PD group was significantly higher than that of Control group at 10 ~ 30 Hz. The PSD of 尾 oscillation in PD group at the 1st week was not significantly different from that in the 0 ~ (th) week. P0.05 decreased significantly at the 2nd / 3rd to 4th week (P 0.05). Conclusion exercise intervention regulates the functional activity of motor cortex. The abnormal 尾 oscillation in M1 region was inhibited, and then its gait and autonomous activity were improved. It is speculated that the abnormal 尾 oscillation in M1 region of PD model rats mediated the synaptic plasticity of cortex-striatal Glu. The motor coding information inputted to the basal ganglia can improve the behavioral function of rats.
【作者單位】： 北京師范大學(xué)體育與運(yùn)動學(xué)院;
【基金】：國家自然科學(xué)基金項(xiàng)目(31571221) 北京市自然科學(xué)基金項(xiàng)目(5142012)
【分類號】：G804.2;R-332

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