本文關(guān)鍵詞： 程序性細(xì)胞死亡受體- T淋巴細(xì)胞 炎癥　出處：《免疫學(xué)雜志》2017年06期 　論文類型：期刊論文

【摘要】：目的探討在LPS模擬的炎癥環(huán)境中,老年人T淋巴細(xì)胞功能與程序性細(xì)胞死亡受體-1(PD-1)表達(dá)的相關(guān)性。方法選取我院健康體檢人員110例,其中年輕人55例(65歲),老年人55例(≥65歲)。流式細(xì)胞術(shù)檢測(cè)外周血T淋巴細(xì)胞亞群(CD45-Per CP/CD3-APC/CD8-FITC)數(shù)目及其PD-1(CD279-PE)表達(dá)水平。分離外周血單個(gè)核細(xì)胞,分為對(duì)照組、LPS刺激組、LPS+抗PD-1組,CCK-8法和流式凋亡檢測(cè)術(shù)檢測(cè)細(xì)胞活性及凋亡影響,ELISA法檢測(cè)培養(yǎng)上清中TNF-α、IL-6、IL-10、IL-2和IFN-γ表達(dá)水平。結(jié)果與年輕人相比,老年人各T淋巴細(xì)胞亞群絕對(duì)值均顯著下降,PD-1表達(dá)水平升高(P0.05)。老年人LPS+抗PD-1組較LPS組細(xì)胞活性升高,凋亡率下降(P0.05),年輕人LPS+抗PD-1組與LPS組之間的細(xì)胞活性和凋亡率差異均無(wú)統(tǒng)計(jì)學(xué)意義(P0.05)。老年人LPS組TNF-α表達(dá)水平顯著低于年輕人,IL-10/TNF-α比值高于年輕人(P0.05)。結(jié)論炎癥狀態(tài)下,老年人T淋巴細(xì)胞功能減低與PD-1高表達(dá)密切相關(guān)。免疫應(yīng)答時(shí)淋巴細(xì)胞抑制性細(xì)胞因子分泌增多,更易處于免疫抑制狀態(tài),與老年人較年輕人更易罹患感染性疾病密切相關(guān)。
[Abstract]:Objective to investigate the correlation between the function of T lymphocytes and the expression of programmed cell death receptor (PD-1) in the inflammatory environment simulated by LPS. Of these, 55 were young people aged 65). The number of CD45-Per / CD3-APC / CD8-FITC-1 and the number of CD45-Per / CD3-APC / CD8-FITC in peripheral blood T lymphocyte subsets were detected by flow cytometry in 55 elderly patients (鈮，

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