本文關(guān)鍵詞： 肺疾病 慢性阻塞性 膠原 基質(zhì)金屬蛋白酶 金屬蛋白酶抑制物 熱休克蛋白　出處：《中華臨床醫(yī)師雜志(電子版)》2016年22期 　論文類型：期刊論文

【摘要】：目的探討慢性阻塞性肺疾病(COPD)大鼠模型中氣道彈性及炎癥因子的改變。方法將40只Wistar大鼠隨機(jī)分為5組(G1~G5),每組再分兩組(COPD模型組及正常對(duì)照組),采用煙熏加氣管內(nèi)注入脂多糖的方法制造COPD大鼠模型。用HE染色及馬松(Masson)三色染色評(píng)價(jià)大鼠氣道壁病理變化和膠原纖維的變化;采用ELISA法檢測(cè)血清中基質(zhì)金屬蛋白酶9(MMP9)、金屬蛋白酶抑制物1(TIMP1)及熱休克蛋白27(HSP27)的表達(dá)水平。COPD模型組血清MMP9、TIMP1及HSP27表達(dá)水平的各組間比較采用方差分析,組間兩兩比較采用LSD-t檢驗(yàn),P0.05為有統(tǒng)計(jì)學(xué)差異。結(jié)果 G1、G4、G5組中COPD模型組血清MMP9和TIMP1的表達(dá)水平高于正常對(duì)照組的表達(dá)水平,各COPD模型組血清HSP27的表達(dá)水平均高于正常對(duì)照組;COPD模型組MMP9的表達(dá)總體呈上升趨勢(shì),差異有統(tǒng)計(jì)學(xué)意義(F=17.491,P0.05);COPD模型組TIMP1的表達(dá)總體呈上升趨勢(shì),G3與G5組(P=0.000 0470.001)及G4與G5組(P=0.000 0280.001)差異有統(tǒng)計(jì)學(xué)意義;COPD模型組MMP9/TIMP1的比值隨MMP9和TIMP1改變而變化,差異有統(tǒng)計(jì)學(xué)意義(F=15.076,P0.05);COPD模型組HSP27的表達(dá)呈輕微上升趨勢(shì),G4與G5組之間差異有統(tǒng)計(jì)學(xué)意義(P=0.0270.05)。結(jié)論 MMP9/TIMP1的平衡及膠原纖維、HSP27的改變可以作為評(píng)價(jià)COPD進(jìn)展的重要指標(biāo)。
[Abstract]:Objective to investigate the changes of airway elasticity and inflammatory factors in rats with chronic obstructive pulmonary disease (Wistar). Methods 40 Wistar rats were randomly divided into 5 groups. Each group was divided into two groups: COPD model group and normal control group. The rat model of COPD was established by fumigation and intratracheal injection of lipopolysaccharide. The pathological changes of airway wall and the changes of collagen fibers were evaluated by HE staining and Ma Song Masson staining. Serum matrix metalloproteinase 9 (MMP9) was determined by ELISA. The expression level of metalloproteinase inhibitor 1 (TIMP 1) and heat shock protein 27 (HSP27). The expression level of TIMP1 and HSP27 were analyzed by ANOVA, and LSD-t test was used to compare the expression levels of G1G4 and G1G4. The expression of serum MMP9 and TIMP1 in the COPD model group was higher than that in the normal control group, and the expression level of serum HSP27 in each COPD model group was higher than that in the normal control group. The expression of MMP9 in COPD model group showed an upward trend, and the difference was statistically significant (P 0.05). The expression of TIMP1 in COPD model group showed a general upward trend. There was significant difference between G3 and G5 group (0.000 0470.001) and G4 and G5 group (0.000 0280.001). In COPD model group, the ratio of MMP9/TIMP1 changed with the changes of MMP9 and TIMP1, and the difference was statistically significant (P 0.05). The expression of HSP27 in COPD model group showed a slight upward trend. The difference between G4 and G5 groups was statistically significant (P < 0.05). Conclusion the balance of MMP9/TIMP1 and collagen fibers are significant. The change of HSP27 can be used as an important index to evaluate the progress of COPD.
【作者單位】： 揚(yáng)州大學(xué)第二臨床學(xué)院影像科;天津市人民醫(yī)院影像中心;東南大學(xué)附屬中大醫(yī)院放射科;
【基金】：揚(yáng)州市社會(huì)發(fā)展科技攻關(guān)計(jì)劃立項(xiàng)課題(2012136)
【分類號(hào)】：R563.9;R-332
【正文快照】： 慢性阻塞性肺疾病(chronic obstructive pulmonarydisease,COPD)是一種高患病率、高死亡率的呼吸系統(tǒng)疾病,目前位居全球死因的第4位。在未來幾十年內(nèi),COPD仍是一項(xiàng)嚴(yán)重的公共衛(wèi)生問題,預(yù)計(jì)到2020年COPD將是全球第5位負(fù)擔(dān)最重的疾病及第3位致死性疾病。我國(guó)COPD患病人群中每年

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