本文關(guān)鍵詞：肥胖小鼠良性前列腺增生模型的建立　出處：《中國老年學雜志》2017年19期 　論文類型：期刊論文

  更多相關(guān)文章： 肥胖小鼠 良性前列腺增生 前列腺體積 前列腺指數(shù)

【摘要】：目的構(gòu)建肥胖小鼠良性前列腺增生的動物模型。方法 SPF級C57BL/6J小鼠28只,取8只小鼠作為對照組(C組)喂養(yǎng)普通飼料,剩余的20只小鼠為高脂誘導(dǎo)肥胖組(Dio組)喂食高脂飼料,構(gòu)建肥胖小鼠良性前列腺增生的模型。酶聯(lián)免疫法檢測血糖血脂、血清雌二醇(E2)和睪酮(T);水取代法檢測前列腺體積;HE染色檢測前列腺組織病理變化;IPP圖像分析軟件統(tǒng)計前列腺腺腔面積和上皮高度。結(jié)果實驗第9周,高脂喂養(yǎng)后有12只小鼠達到肥胖標準(為Ob組),肥胖率為60%。Ob組小鼠體重、內(nèi)臟脂肪重量明顯大于C組(P0.05),前列腺重量、前列腺指數(shù)、體積、面積和上皮高度明顯大于C組(P0.05);Ob組E2水平高于C組(P0.05),T水平低于C組(P0.05),而雌雄激素(E2/T)比值Ob組大于C組(P0.05);前列腺組織病理切片顯示Ob組小鼠前列腺發(fā)生增生的病理變化。結(jié)論肥胖小鼠發(fā)生了前列腺增生。
[Abstract]:Objective to construct the animal model of benign prostatic hyperplasia in obese mice. Methods SPF 28 C57BL/6J mice, 8 mice as control group (group C) were fed with common feed, the remaining 20 mice for high fat induced obesity group (Dio group) were fed a high-fat diet, construct obese mice benign prostatic hyperplasia model detection. Blood glucose and lipid ELISA, serum estradiol (E2) and testosterone (T); water substitution method detection of prostate volume; HE staining was used to detect the pathological changes of prostate tissue; IPP image analysis software statistical prostate cavity area and epithelial height. The results of experiment ninth weeks after high-fat feeding 12 mice obese (for the Ob group), the obesity rate was 60%.Ob mice body weight, visceral fat weight was significantly higher than that of C group (P0.05), prostate weight, prostate index, volume, area and epithelial height was significantly higher than that of C group (P0.05); the level of E2 Ob was higher than that of C group (P0.05), T Compared with group C (P0.05), the ratio of sex hormones (E2/T) in group Ob was larger than that in group C (P0.05), and pathological changes in prostate tissue showed that there was a pathological change in hyperplasia of prostate in Ob group.

【作者單位】： 上海體育學院上海市人類運動能力開發(fā)與保障重點實驗室運動健身科技省部重點實驗室;
【基金】：國家自然科學基金資助項目(81472148) 上海市體育局科技綜合計劃項目(Z022)
【分類號】：R-332;R697.3
【正文快照】： 良性前列腺增生(BPH)是老年男性最常見的疾病之一,年齡、生活方式等是BPH的重要原因,60~69歲的男性BPH發(fā)病率為70%,70歲以上的男性BPH的發(fā)病率為80%[1]。因此,探索BPH的發(fā)病機制,對提高老年男性的生活質(zhì)量具有重大意義。國內(nèi)外多項研究發(fā)現(xiàn)肥胖與BPH密切相關(guān),體重、身高、腰圍，

本文編號：1418577