本文關鍵詞：高表達人源Her2乳腺癌小鼠模型的建立及鑒定　出處：《第二軍醫(yī)大學學報》2017年04期 　論文類型：期刊論文

  更多相關文章： 乳腺腫瘤 轉基因小鼠 動物模型 Her

【摘要】：目的構建并鑒定高表達人源Her2乳腺癌轉基因小鼠模型。方法構建pMD18T-MMTV-huHER2-EGFP轉基因構件,通過原核顯微注射方法將線性化、純化后的外源質粒pMD18T-MMTV-huHER2-EGFP注射入C57BL/6J小鼠受精卵中,胚胎移植至同期發(fā)情的假孕受體母鼠輸卵管內,獲得子代小鼠。采用PCR鑒定子代小鼠尾部組織基因組DNA,用蛋白質免疫印跡法檢測乳腺組織Her2蛋白的表達,通過組織病理學切片觀察轉基因小鼠乳腺組織的病理學變化。結果經PCR方法檢測得到轉基因陽性小鼠。蛋白質免疫印跡結果顯示,與野生型小鼠相比,F2代陽性小鼠的乳腺組織中Her2蛋白高表達。病理組織切片表明25周齡陽性小鼠的乳腺組織已經出現明顯的癌變傾向。結論高表達人源Her2乳腺癌小鼠模型成功建立并能穩(wěn)定遺傳,可自發(fā)形成乳腺癌,其生物學特性和病理學改變與人乳腺癌相近,可作為研究乳腺癌發(fā)生發(fā)展的動物模型。
[Abstract]:Objective to construct and identify the high expression of human breast cancer Her2 transgenic mouse model. Methods we constructed pMD18T-MMTV-huHER2-EGFP transgenic components by pronuclear microinjection method will be linear, the plasmid pMD18T-MMTV-huHER2-EGFP C57BL/6J was injected into mouse zygotes after purification, embryo transfer to pseudopregnant pseudopregnant female fallopian tube of estrus, the offspring were obtained. The genomic DNA PCR identification of offspring mouse tail tissue expression using Western blot analysis of Her2 protein in breast tissue, pathological changes were observed in transgenic mice mammary gland tissues by histological sections. The results detected by PCR from transgenic mice. Western blot results showed that, compared with wild-type mice, Her2 protein the expression of F2 positive mouse mammary tissues. Pathological sections showed that breast group 25 week old mice positive fabric There has been a noticeable tendency of canceration. Conclusion the highly expressed mouse model of human Her2 breast cancer is successfully established and stable, which can spontaneously form breast cancer. Its biological characteristics and pathological changes are similar to those of human breast cancer. It can be used as an animal model to study the occurrence and development of breast cancer.

【作者單位】： 浙江嘉興學院醫(yī)學院醫(yī)學實驗中心;浙江嘉興學院醫(yī)學院藥學系;第二軍醫(yī)大學海軍醫(yī)學系放射醫(yī)學教研室;
【基金】：浙江省科技廳項目(2014C37018,2017C37118,2016C37097)~~
【分類號】：R737.9;R-332
【正文快照】： 乳腺癌是女性惡性腫瘤死亡威脅的第二大元兇,需耗費巨額的人力、物力、財力用于乳腺癌治療的研究[1]。研發(fā)一種新的乳腺癌藥物,平均要花費6.1億美元,還要進行為期37個月的動物實驗[2]。因此,研究者們前期會應用乳腺癌動物模型來篩選有效的治療方法,以期為后續(xù)的臨床試驗提供強

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1 張慧影;抗雌激素藥物治療乳腺癌機理的探討[J];生理科學;1985年04期

2 Lippman ME;李春華;;乳腺癌的自體和旁分泌生長調節(jié)[J];國外醫(yī)學.婦產科學分冊;1988年01期

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4 李艷;張海燕;王U，

本文編號：1373723