本文關(guān)鍵詞：膀胱癌5637細(xì)胞系的體外培養(yǎng)及荷瘤裸鼠實(shí)驗(yàn)動物模型的建立　出處：《北京協(xié)和醫(yī)學(xué)院》2012年博士論文　論文類型：學(xué)位論文

  更多相關(guān)文章： 膀胱癌 5637細(xì)胞系 體外培養(yǎng) 生長曲線 動物模型

【摘要】：背景：膀胱癌是泌尿系統(tǒng)最常見的腫瘤,臨床上通�？煞譃榫窒抻谡衬さ臏\表腫瘤和肌層浸潤性腫瘤。治療肌層浸潤性膀胱癌的重要手段是全身化療,但目前常用的化療方案(GC、MVAC)療效不佳,而且毒副作用比較嚴(yán)重。針對以上這些缺點(diǎn),一種新的治療方法開始被大家所重視,這就是分子靶向治療。該方法具有選擇性高、毒副作用小的特點(diǎn)。為了能夠很好地評價靶向藥物的療效,最常用的方法就是建立膀胱癌的動物模型進(jìn)行藥物試驗(yàn)。 目的：了解膀胱癌5637細(xì)胞系在體外的生長情況,并且建立動物模型以備進(jìn)行藥物試驗(yàn)。 方法：取凍存的膀胱癌5637細(xì)胞進(jìn)行復(fù)蘇并傳代培養(yǎng),待細(xì)胞生長旺盛且形態(tài)較好時進(jìn)行生長曲線的測定。將處于對數(shù)生長期的膀胱癌細(xì)胞制成細(xì)胞懸液,注射入2只裸鼠皮下,數(shù)周后取出腫瘤,將其切成小塊移植入4只裸鼠皮下,觀察腫瘤生長情況并測定腫瘤大小,取腫瘤組織進(jìn)行HE染色后光鏡下觀察其結(jié)構(gòu)。 結(jié)果：膀胱癌5637細(xì)胞傳代培養(yǎng)后生長速度良好。加入CCK-8試劑后,每日測定吸光度,以時間為橫軸,吸光度為縱軸,繪制出生長曲線。結(jié)果顯示,膀胱癌細(xì)胞d1-4處于對數(shù)生長期,增長速度快,活性佳,d4之后進(jìn)入平臺期。將膀胱癌細(xì)胞懸液注射入2只裸鼠皮下后約2-3周達(dá)到最大體積,之后將腫瘤組織塊移植入4只裸鼠皮下,約2周腫瘤達(dá)到最大體積。 結(jié)論：荷膀胱癌裸鼠異位移植瘤模型成瘤率高,生長迅速,腫瘤大小受實(shí)驗(yàn)操作影響較大。
[Abstract]:Background: bladder cancer is the most common tumor in urinary system, often clinically can be divided into the superficial tumors confined to the mucosa and muscle invasive tumor. Treatment of muscle invasive bladder cancer is an important means of systemic chemotherapy, but the common chemotherapy (GC, MVAC) poor efficacy and toxic side effects more serious. In view of the above shortcomings, a new method of treatment began to be valued, this is the molecular targeted therapy. This method has the advantages of high selectivity, small toxic side effect. In order to effectively evaluate the efficacy of drugs targeting the animal model, the most commonly used method is to establish the bladder cancer drug testing.
Objective: to understand the growth of the 5637 cell line of bladder cancer in vitro, and to establish an animal model for the drug test.
Methods: the recovery of cryopreserved and cultured from 5637 bladder cancer cells, when cell growth Washingce good morphology were determined. The growth curve of the logarithmic phase of growth of bladder cancer cells into cell suspension was injected into 2 nude mice, a few weeks after the tumor removed, cut it into pieces transplanted into 4 nude mice, growth of tumor was observed and measured tumor size, tumor tissues were observed with HE staining and its structure.
Results: the passage of bladder cancer 5637 cell culture growth speed is good. After adding CCK-8 reagent, the daily measurement of absorbance with time as the horizontal axis, the absorbance for the vertical axis, the growth curve was drawn. The results showed that bladder cancer D1-4 cells in logarithmic growth phase, fast growth, good activity, D4 after bladder cancer into the platform. The maximum volume of cell suspension was injected into the liquid reaches about 2-3 weeks after 2 nude mice, tumor tissue transplanted into 4 nude mice, tumor volume reached a maximum of about 2 weeks.
Conclusion: the ectopic tumor model of nude mice with bladder cancer has a high tumor rate and rapid growth, and the size of the tumor is greatly influenced by the experimental operation.

【學(xué)位授予單位】：北京協(xié)和醫(yī)學(xué)院
【學(xué)位級別】：博士
【學(xué)位授予年份】：2012
【分類號】：R737.14;R-332

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本文編號：1358896