發(fā)布時間：2017-12-27 10:10

  本文關(guān)鍵詞：結(jié)核分枝桿菌panD蛋白功能預(yù)測及生物信息學(xué)分析　出處：《中國病原生物學(xué)雜志》2017年06期 　論文類型：期刊論文

  更多相關(guān)文章： 結(jié)核分枝桿菌 panD 抗原表位 耐藥 生物信息學(xué)

【摘要】：目的研究結(jié)核分枝桿菌panD(Rv3601c)基因及其編碼蛋白的結(jié)構(gòu)和功能,為耐藥結(jié)核的治療提供參考依據(jù)。方法運用ClustalX 2.1、MEGA 6.06軟件以及ExPASy、NCBI等在線工具分析結(jié)核分枝桿菌panD基因信息,同時分析其進(jìn)化特征、編碼蛋白質(zhì)的理化性質(zhì)、信號肽、磷酸化位點、結(jié)構(gòu)特點并預(yù)測其功能;利用BepiPred 1.0Server和NetMHCIIpan 3.1Server預(yù)測panD蛋白的B細(xì)胞和T細(xì)胞抗原表位。結(jié)果結(jié)核分枝桿菌panD基因全長420bp,不同菌株panD基因序列相似度100%,具有高度同源性,進(jìn)化關(guān)系較近,編碼139個氨基酸。panD蛋白不穩(wěn)定系數(shù)為8.72,親水性平均系數(shù)為0.147,為穩(wěn)定、疏水性蛋白、無跨膜區(qū)與信號肽,存在9個磷酸化位點。二級結(jié)構(gòu)中以無規(guī)則卷曲為主,結(jié)構(gòu)較疏松。三級結(jié)構(gòu)同源建模成功。該蛋白具有多個潛在的B細(xì)胞抗原表位和T細(xì)胞抗原表位。結(jié)論panD蛋白作為脫羧酶,在結(jié)核分枝桿菌合成β-丙氨酸過程中具有重要作用。panD蛋白序列保守穩(wěn)定,具有多個優(yōu)勢抗原表位,是治療耐藥結(jié)核的潛在新靶標(biāo)。
[Abstract]:Objective to study the structure and function of Mycobacterium tuberculosis panD (Rv3601c) gene and its encoded protein, so as to provide reference for the treatment of drug-resistant tuberculosis. Methods using ClustalX 2.1 and MEGA 6.06 software and ExPASy, NCBI and other online tools to analyze the genetic information of Mycobacterium tuberculosis panD, and analyzes its evolution characteristics, encoding protein physicochemical properties, signal peptide, phosphorylation sites, the structure characteristics and predict its function; prediction of panD protein by BepiPred 1.0Server and NetMHCIIpan 3.1Server and T B cells cell epitope. Results the panD gene of Mycobacterium tuberculosis was 420bp, and the sequence similarity of different panD genes was 100%, which was highly homologous. The evolutionary relationship was close, encoding 139 amino acids. The panD protein instability coefficient was 8.72, the average hydrophilic coefficient was 0.147, which was stable, hydrophobic protein, transmembrane free zone and signal peptide, and there were 9 phosphorylation sites. The two stage structure is dominated by irregular curling, and the structure is loose. Three level structure homologous modeling is successful. The protein has multiple potential B cell epitopes and T cell epitopes. Conclusion panD protein, as decarboxylase, plays an important role in the synthesis of beta - alanine in Mycobacterium tuberculosis. The panD protein sequence is conservative and stable, and has multiple dominant epitopes. It is a potential new target for the treatment of drug-resistant tuberculosis.
【作者單位】： 濟(jì)寧醫(yī)學(xué)院臨床醫(yī)學(xué)院;濰坊醫(yī)學(xué)院;濰坊醫(yī)學(xué)院臨床醫(yī)學(xué)院;
【基金】：國家自然科學(xué)基金項目(No.30972639) 山東省自然科學(xué)基金項目(No.ZR2016HM09)
【分類號】：R378.911
【正文快照】： 結(jié)核病是由胞內(nèi)寄生菌-結(jié)核分枝桿菌(Mycobac-terium tuberculosis,MTB)引起的傳染病。隨著近幾年抗生素的濫用和潛伏感染、耐藥結(jié)核的出現(xiàn),使結(jié)核***病的根治面臨重重困難。我國是全球22個結(jié)核病高負(fù)擔(dān)國家之一,近年來MTB耐藥、耐多藥情況尤為嚴(yán)重[1]。吡嗪酰胺(pyrazinamide，

本文編號：1341269