本文選題：當(dāng)歸多糖　切入點：雙氧水　出處：《南京醫(yī)科大學(xué)》2017年博士論文　論文類型：學(xué)位論文

【摘要】：目的:本實驗主要研究當(dāng)歸多糖(angelica sinensis polysaccharide,ASP)在雙氧水(hydrogenperoxide,H_2O_2)所誘導(dǎo)的大鼠軟骨細(xì)胞氧化應(yīng)激損傷中所起的作用,并探討其作用機制。方法:取SD大鼠雙側(cè)膝關(guān)節(jié)軟骨細(xì)胞行體外分離培養(yǎng),在體外培養(yǎng)的軟骨細(xì)胞中加入H_2O_2,建立大鼠軟骨細(xì)胞氧化應(yīng)激損傷模型。在體外培養(yǎng)的大鼠軟骨細(xì)胞中加入不同濃度的ASP并與H_2O_2共培養(yǎng),通過檢測大鼠軟骨細(xì)胞的細(xì)胞活性、細(xì)胞凋亡水平、腫瘤壞死因子-α(tumournecrosis factor-α,TNF-α)和白細(xì)胞介素-1β(interleukin-1β,IL-1β)水平、超氧化物歧化酶(superoxide dismutase,SOD)和過氧化氫酶(catalase,CAT)活性以及丙二醛(malondialdehyde,MDA)水平來評價ASP的保護作用。同時應(yīng)用實時定量逆轉(zhuǎn)錄聚合酶鏈反應(yīng)(quantitative real-time reverse transcription polymerase chain reaction,qRT-PCR)來估測骨性關(guān)節(jié)炎(osteoarthritis,OA)相關(guān)基因的相對表達(dá)水平,其中包括II型膠原(typeⅡcollagen,Col2a1)、aggrecan、SOX9、基質(zhì)金屬蛋白酶(matrix metalloproteinase,MMP)-1,-3,-9以及組織基質(zhì)金屬蛋白酶抑制劑(tissue inhibitor of matrix metalloproteinase,TIMP)-1。結(jié)果:在H_2O_2所誘導(dǎo)的大鼠軟骨細(xì)胞氧化應(yīng)激損傷中,ASP通過其抗氧化、抗凋亡以及抗炎作用機制在體外對大鼠軟骨細(xì)胞氧化應(yīng)激損傷起保護作用,使大鼠軟骨細(xì)胞免于氧化應(yīng)激所致細(xì)胞損傷。結(jié)論:ASP可作為一種替代性藥物在骨性關(guān)節(jié)炎的臨床治療中起到一定作用。
[Abstract]:Objective: to investigate the role of Angelica sinensis polysaccharide in oxidative stress injury of chondrocytes in rats induced by hydrogen peroxide peroxide (H _ 2O _ 2). Methods: the chondrocytes of bilateral knee joint of SD rats were isolated and cultured in vitro. The oxidative stress injury model of rat chondrocytes was established by adding H _ S _ 2O _ 2 into the chondrocytes cultured in vitro. Different concentrations of ASP were added to the chondrocytes cultured in vitro and co-cultured with H _ 2O _ 2 to detect the cellular activity of rat chondrocytes. The levels of apoptosis, tumor necrosis factor- 偽 (TNF- 偽) and interleukin-1 尾 interleukin-1 尾 (IL-1 尾) were measured. The activity of superoxide dismutase (SOD) and catalase (catalase) and the level of malondialdehyde (MDA) were used to evaluate the protective effect of ASP. The real-time quantitative reverse transcriptase polymerase chain reaction (RT-PCR) was used to estimate the osteoarthritis of osteoarthritis by quantitative real-time transcription polymerase chain reactionation (qRT-PCR). The relative level of expression of the related gene, These include type II collagen type 鈪，

本文編號：1580423