本文選題：Nrf2敲除鼠 + 低氧運(yùn)動��； 參考：《北京體育大學(xué)》2016年博士論文

【摘要】：目的:低氧訓(xùn)練已被廣泛用來提高運(yùn)動員運(yùn)動能力的訓(xùn)練方法。它試圖通過低氧環(huán)境和運(yùn)動雙重因素刺激,使機(jī)體出現(xiàn)強(qiáng)烈的應(yīng)激反應(yīng),最大程度地調(diào)動機(jī)體的潛能,從而產(chǎn)生一系列有助于提高運(yùn)動能力的生理生化反應(yīng),以達(dá)到提高運(yùn)動能力的目的。然而,低氧訓(xùn)練對骨骼肌的抗氧化作用分子機(jī)制,目前并不十分清楚,低氧訓(xùn)練介導(dǎo)Nrf2對骨骼肌抗氧化作用的影響尚未見報(bào)道。因此,本研究通過對Nrf2基因敲除和野生型小鼠進(jìn)行為期4周的低氧運(yùn)動訓(xùn)練,觀察小鼠骨骼肌ROS含量、SOD和CAT抗氧化酶蛋白表達(dá)、GSH/GSSG比值的變化,以及對運(yùn)動能力的影響,從而為低氧訓(xùn)練的訓(xùn)練效果機(jī)制提供一定的理論依據(jù)。實(shí)驗(yàn)方法:采用Nrf2敲除(KO)和野生(WT)小鼠,分別隨機(jī)分為兩組:常氧安靜組和低氧訓(xùn)練組,每組10只。常氧安靜組:小鼠在常氧狀態(tài)下,正�；\中生活,不施加運(yùn)動負(fù)荷;低氧訓(xùn)練組:小鼠每天在常壓低氧環(huán)境(11.2%的氧濃度)中暴露8小時(shí),并在低氧環(huán)境進(jìn)行坡度為0,運(yùn)動速度為12米/分跑臺運(yùn)動1小時(shí),每周6天,連續(xù)四周。最后一次低氧訓(xùn)練后休息48小時(shí),進(jìn)行運(yùn)動能力測定。而后48小時(shí),頸椎脫臼處死,取小鼠骨骼肌,快速置于液氮中,再于-80度凍存。測定骨骼肌ROS、Nrf2mRNA、SOD1、SOD2和CAT蛋白表達(dá)、GSH/GSSG比值和運(yùn)動至力竭的跑距。結(jié)果表明(1)野生鼠和Nrf2敲除鼠四周低氧訓(xùn)練后骨骼肌ROS均顯著性增加;(2)安靜Nrf2敲除鼠Nrf2mRNA、SOD2和CAT蛋白表達(dá)量以及運(yùn)動能力顯著低于野生鼠;(3)四周低氧訓(xùn)練后,與野生鼠相比,Nrf2敲除鼠GSH/GSSG比值顯著性下降和運(yùn)動能力非常顯著性降低。結(jié)論:低氧訓(xùn)練可以提高野生小鼠的抗氧化能力,在Nrf2缺失環(huán)境下,低氧訓(xùn)練對Nrf2敲除鼠其無明顯改善作用。
[Abstract]:Objective: hypoxia training has been widely used to improve the sports ability of athletes. It tries to stimulate the body by the dual factors of low oxygen environment and movement, and make the body have a strong stress response and maximize the potential of the body, thus producing a series of physiological and biochemical reactions that can help to improve the exercise ability, so as to improve the transportation of the body. However, the molecular mechanism of the antioxidant activity of hypoxia training on skeletal muscle is not very clear. The effect of hypoxia training on the antioxidant effect of Nrf2 on skeletal muscle has not yet been reported. Therefore, this study was conducted to observe the R of skeletal muscle in mice by 4 weeks of hypoxia exercise training in Nrf2 knockout and wild type mice. OS content, the expression of SOD and CAT anti oxidase protein, the change of the GSH/GSSG ratio and the effect on the exercise ability, thus providing a certain theoretical basis for the training effect mechanism of hypoxia training. The experimental method: using Nrf2 knockout (KO) and the wild (WT) mice, they were randomly divided into two groups: normoxic quiet group and hypoxia training group, 10 rats in each group. Quiet group: mice in normal oxygen state, normal cage life, no exercise load, low oxygen training group: mice were exposed to 8 hours in the normal pressure and hypoxia environment (11.2% oxygen concentration) every day, and in the low oxygen environment, the slope was 0, and the speed of movement was 12 meters / sub run 1, 6 days a week, four weeks. After the last hypoxia training, 48 rest 48 rest after 48 rest. For hours, exercise ability was measured. After 48 hours, the cervical dislocations were killed, the skeletal muscles were taken, and the mice were quickly placed in the liquid nitrogen and then stored at -80 degrees. The expression of ROS, Nrf2mRNA, SOD1, SOD2 and CAT protein, the GSH/GSSG ratio and the distance of movement to exhaustion were measured. The results showed (1) the skeletal muscle ROS after the hypoxia training of wild and Nrf2 knockout mice. (2) the Nrf2mRNA, SOD2 and CAT protein expression and exercise ability of quiet Nrf2 knockout mice were significantly lower than those of wild mice; (3) after the four weeks of hypoxia training, the GSH/GSSG ratio of Nrf2 knockout mice decreased significantly and the exercise ability was significantly reduced. Conclusion: hypoxia training can improve the antioxidant capacity of wild mice. In the absence of Nrf2, hypoxic training had no significant effect on Nrf2 knockout mice.

【學(xué)位授予單位】：北京體育大學(xué)
【學(xué)位級別】：博士
【學(xué)位授予年份】：2016
【分類號】：G804.7

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本文編號：1873854