發(fā)布時(shí)間：2018-01-06 02:23

  本文關(guān)鍵詞：KLF9在胰腺癌中的表達(dá)及其臨床意義　出處：《江蘇大學(xué)》2017年碩士論文　論文類型：學(xué)位論文

  更多相關(guān)文章： 胰腺癌 Krüppel樣轉(zhuǎn)錄因子 預(yù)后

【摘要】：目的:本實(shí)驗(yàn)通過研究KLF9(Krüppel樣轉(zhuǎn)錄因子9)在胰腺癌中的表達(dá)并研究其與胰腺癌患者臨床病理參數(shù)及預(yù)后的關(guān)系,從而為胰腺癌的診治提供新的研究思路。方法:本實(shí)驗(yàn)通過采集150例胰腺癌及其配對(duì)的癌旁組織標(biāo)本,從中隨機(jī)挑選28對(duì)標(biāo)本運(yùn)用實(shí)時(shí)熒光定量PCR(RT-PCR)技術(shù),以了解胰腺癌及其配對(duì)的癌旁組織中的KLF9 m RNA的表達(dá)情況;隨機(jī)挑選8對(duì)標(biāo)本采用蛋白印跡技術(shù)(Western Blot)技術(shù),并對(duì)所有的150對(duì)標(biāo)本運(yùn)用免疫組化技術(shù)檢測(cè)胰腺癌及其配對(duì)癌旁組織中的KLF9蛋白的表達(dá)情況,并統(tǒng)計(jì)分析KLF9的表達(dá)水平與病人臨床病理參數(shù)及生存率的關(guān)系。結(jié)果:實(shí)時(shí)熒光定量PCR結(jié)果顯示,在28個(gè)配對(duì)的胰腺癌標(biāo)本中,與癌旁正常胰腺組織相比,胰腺癌組織中KLF9 mRNA的表達(dá)水平明顯下調(diào)(21/28)。蛋白免疫印跡結(jié)果顯示,與癌旁正常對(duì)照胰腺組織相比,胰腺癌組織中KLF9蛋白表達(dá)水平明顯下降。胰腺癌組織基因芯片(含150對(duì)胰腺癌組織和配對(duì)的正常的胰腺組織)免疫組化結(jié)果顯示,KLF9在胰腺癌組織中的染色強(qiáng)度明顯減弱。KLF9與病人臨床病理參數(shù)分析顯示,KLF9的表達(dá)水平與腫瘤的分化程度(p=0.008)和血管侵犯(p=0.006)顯著相關(guān)。生存分析顯示,KLF9陽性表達(dá)患者1、2、3年總生存率明顯高KLF9陰性表達(dá)患者(41%、31%、16%vs.18%、6%、6%)、中位生存時(shí)間明顯長于KLF9陰性表達(dá)患者(21.05個(gè)月vs.11.82個(gè)月)(均P0.05)。結(jié)論:通過RT-PCR、蛋白質(zhì)免疫印跡、免疫組化技術(shù)顯示KLF9在胰腺癌組織中低表達(dá),其表達(dá)水平與腫瘤的分化程度及血管侵犯顯著相關(guān),同時(shí)KLF9與胰腺癌患者的生存時(shí)間也有相關(guān)性,由此可猜測(cè)KLF9可能介入了胰腺癌的發(fā)生發(fā)展過程。
[Abstract]:Objective: To investigate the effect of KLF9 (Kr ppel like transcription factor 9) expression in pancreatic carcinoma and to study its relationship with clinical pathological parameters and prognosis of patients with pancreatic cancer, so as to provide a new idea for the diagnosis and treatment of pancreatic cancer. Methods: This study collected 150 cases of pancreatic cancer and its adjacent tissue samples from 28 randomly selected specimens by real-time fluorescence quantitative PCR (RT-PCR) technology, the expression of KLF9 m RNA for pancreatic cancer and adjacent tissues; 8 randomly selected specimens using Western blot technique (Western Blot) technology, and the expression of all 150 samples on the use of immunohistochemical detection of pancreatic cancer and its adjacent tissues KLF9 protein, and statistical analysis of the expression of KLF9 and clinicopathological parameters and patient survival rate. Results: the results of real-time quantitative PCR showed that in 28 Pancreatic cancer specimens paired, compared with adjacent normal pancreatic tissue, the expression of KLF9 in pancreatic cancer tissues of mRNA was significantly reduced (21/28). Western blot results showed that compared with adjacent normal pancreatic tissue in the control, decreased level of expression of KLF9 protein in pancreatic cancer. Pancreatic cancer tissue microarray (including 150 in pancreatic cancer tissues and paired normal pancreatic tissue) immunohistochemistry showed that the staining intensity of KLF9 in pancreatic cancer patients significantly decreased.KLF9 and clinicopathological parameters analysis showed that KLF9 expression and tumor differentiation degree of (p=0.008) and vascular invasion (p=0.006). Survival analysis showed significant correlation, KLF9 the positive expression of 1,2,3 in patients with 5-year overall survival rate was significantly higher in patients with negative expression of KLF9 (41%, 31%, 16%vs.18%, 6%, 6%), the median survival time was significantly longer than that in patients with negative expression of KLF9 (21.05 vs.11.82 months) (P0. 05). Conclusion: by RT-PCR, Western blot, immunohistochemistry showed low expression of KLF9 in pancreatic cancer tissue, differentiation and vascular invasion of the tumor and its expression level was significantly related to patients with pancreatic cancer and KLF9 survival time is related to the speculation that KLF9 might be involved in the development and progression of pancreatic cancer process.

【學(xué)位授予單位】：江蘇大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2017
【分類號(hào)】：R735.9

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本文編號(hào)：1385916