本文關(guān)鍵詞：HMGB1蛋白在扁平苔蘚發(fā)病機(jī)制中的作用　出處：《吉林大學(xué)》2017年碩士論文　論文類型：學(xué)位論文

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【摘要】：背景:扁平苔蘚是一種病因尚未明確的慢性炎癥性皮膚病,細(xì)胞免疫在其發(fā)病中起重要作用。細(xì)胞外的HMGB1蛋白作為損傷相關(guān)模式分子的代表,在多種炎癥和自身免疫性疾病的發(fā)病機(jī)制中發(fā)揮重要作用,但其在扁平苔蘚發(fā)病機(jī)制中的作用未見報(bào)道。目的:觀察扁平苔蘚及正常人皮膚組織HMGB1蛋白的表達(dá)情況進(jìn)行檢測(cè),探討HMGB1蛋白在扁平苔蘚發(fā)病機(jī)制中的作用方法:免疫組化檢測(cè)扁平苔蘚及正常人皮膚組織中HMGB1蛋白表達(dá)及分布情況,比較不同類型扁平苔蘚HMGB1的表達(dá)分布,比較初期、成熟期及消退期扁平苔蘚HMGB1的表達(dá)分布,比較扁平苔蘚皮損內(nèi)、皮損邊緣及皮損外正常皮膚皮膚的表達(dá)分布,并分析HMGB1在表皮角質(zhì)形成細(xì)胞核內(nèi)的陽性率與真皮炎性細(xì)胞浸潤程度的相關(guān)性。結(jié)果:1.HMGB1蛋白在扁平苔蘚表皮細(xì)胞胞核的陽性細(xì)胞比例較正常皮膚降低,細(xì)胞漿、細(xì)胞外間隙陽性分布較正常皮膚顯著升高(P0.05),陽性細(xì)胞以表皮基底層和棘層中下部為主。2.HMGB1蛋白在扁平苔蘚不同亞型(急性泛發(fā)性扁平苔蘚,慢性局限性扁平苔蘚、口唇扁平苔蘚、肥厚性扁平苔蘚、毛囊性扁平苔蘚、反向性扁平苔蘚、色素性扁平苔蘚、萎縮性扁平苔蘚)都具有上述扁平苔蘚的共同特點(diǎn)。但也有差異:急性泛發(fā)性扁平苔蘚表皮較角質(zhì)形成細(xì)胞核陽性率較其他亞型高;色素性扁平苔蘚、萎縮性扁平苔蘚胞漿及細(xì)胞外間隙分布較其他亞型低。3.HMGB1在不同時(shí)期扁平苔蘚的分布:HMGB1蛋白在不同時(shí)期扁平苔蘚皮損表皮細(xì)胞的表達(dá)分布各有差異。初期、成熟期扁平苔蘚皮損表皮細(xì)胞核陽性率較消退期扁平苔蘚低,但初期扁平苔蘚皮損表皮細(xì)胞漿及細(xì)胞外間隙陽性率較成熟期扁平苔蘚和消退期扁平苔蘚高,且成熟期扁平苔蘚表皮細(xì)胞漿及細(xì)胞外間隙陽性率較消退期扁平苔蘚高。以上差異均有統(tǒng)計(jì)學(xué)意義(P0.05)。4.HMGB1蛋白在扁平苔蘚皮損不同部位細(xì)胞核、細(xì)胞漿及細(xì)胞外間質(zhì)的表達(dá)百分比,細(xì)胞核:皮損內(nèi)皮損邊緣皮損外正常皮膚;細(xì)胞漿:皮損邊緣較皮損外正常皮膚和皮損內(nèi)增高;細(xì)胞外間隙:皮損邊緣較皮損外正常皮膚和皮損內(nèi)增高。所有差異都具顯著性(P0.05)。5.HMGB1在表皮角質(zhì)形成細(xì)胞核的陽性率與真皮炎性細(xì)胞浸潤數(shù)呈負(fù)相關(guān),(y=-25.58X+1507 R=-0.853,p0.001),即表皮內(nèi)細(xì)胞細(xì)胞核陽性率越低,真皮內(nèi)炎性細(xì)胞浸潤數(shù)越多。結(jié)論:1.與正常皮膚相比,扁平苔蘚皮損中HMGB1核表達(dá)降低,胞漿、細(xì)胞外間隙表達(dá)升高,且在扁平苔蘚發(fā)病初期和皮損邊緣更顯著,提示HMGB1由胞核向胞漿、細(xì)胞外間隙的轉(zhuǎn)移可能與扁平苔蘚炎癥發(fā)生有關(guān);2.HMGB1在扁平苔蘚表皮角質(zhì)形成細(xì)胞核陽性率與真皮淋巴細(xì)胞浸潤數(shù)呈負(fù)相關(guān),進(jìn)一步提示HMGB1由胞核向胞漿、細(xì)胞外間隙的轉(zhuǎn)移由與扁平苔蘚真皮淋巴細(xì)胞浸潤相關(guān)。
[Abstract]:Background: lichen planus is a chronic inflammatory skin disease with unknown etiology. Cellular immunity plays an important role in the pathogenesis of lichen planus. Extracellular HMGB1 protein is the representative of damage related model molecules. It plays an important role in the pathogenesis of many kinds of inflammation and autoimmune diseases. But its role in the pathogenesis of lichen planus has not been reported. Objective: to observe the expression of HMGB1 protein in lichen planus and normal human skin. To investigate the role of HMGB1 protein in the pathogenesis of lichen planus methods: immunohistochemistry was used to detect the expression and distribution of HMGB1 protein in lichen planus and normal human skin. To compare the expression distribution of HMGB1 in different types of lichen planus, the expression distribution of HMGB1 in early stage, mature stage and extinction stage, and compare the expression distribution of HMGB1 in the lesions of lichen planus. The distribution of the expression of normal skin on the edge of the lesion and the skin outside the lesion. The relationship between the positive rate of HMGB1 in keratinocytes and the degree of dermal inflammatory cell infiltration was analyzed. 1. The proportion of HMGB1 protein positive cells in the nucleus of lichen planus epidermis cells was lower than that in normal skin. The positive distribution of cytoplasm and extracellular space was significantly higher than that of normal skin (P0.05). The positive cells were mainly epidermal basal layer and middle and lower part of spinous layer. 2. HMGB1 protein was found in different subtypes of lichen planus (acute generalized lichen planus, chronic localized lichen planus, oral lichen planus). Hypertrophic lichen planus, follicular lichen planus, reverse lichen planus, and pigmented lichen planus. Atrophic lichen planus) all have the same characteristics of the above lichen planus, but there are also differences: the positive rate of keratinocyte in acute generalized lichen planus is higher than that in other subtypes. Luteous lichen planus. The distribution of cytoplasm and extracellular space in atrophic lichen planus was lower than that in other subtypes. 3. The distribution of HMGB1 in different stages of lichen planus:. The expression and distribution of HMGB1 protein in epidermal cells of lichen planus were different at different stages. The positive rate of epidermal nuclei in mature lichen planus was lower than that in dissipated lichen planus, but the positive rate of epidermal cytoplasm and extracellular space in primary lichen planus was higher than that in mature lichen planus and extinction lichen planus. The positive rates of epidermal cytoplasm and extracellular space in mature lichen planus were higher than those in dissipated lichen planus. 4. HMGB1 protein was located in the nuclei of different parts of lichen planus lesions. Percentage of expression of cytoplasm and extracellular stroma, nucleus: normal skin outside the edge of skin lesion; Cytoplasm: the edge of the lesions was higher than that of the normal skin and the lesions. Extracellular space: the edge of the lesions was higher than that of the normal skin and the lesions. All the differences were significant (P0.05). The positive rate of HMGB1 in keratinocytes was negatively correlated with the number of dermal inflammatory cells. The positive rate of cell nucleus in epidermis was lower than that in the epidermis. Conclusion 1. Compared with normal skin, the expression of HMGB1 in the lesions of lichen planus decreased, and the expression of cytoplasm and extracellular space increased. Moreover, it was more obvious in the early stage of lichen planus and the edge of lesions, suggesting that the transfer of HMGB1 from nucleus to cytoplasm and the transfer of extracellular space may be related to the occurrence of lichen planus inflammation. 2. The positive rate of HMGB1 in epidermal keratinocytes of lichen planus was negatively correlated with the number of dermal lymphocyte infiltration, which further suggested that HMGB1 changed from nucleus to cytoplasm. Metastasis of the extracellular space is associated with lymphocytic infiltration in the dermis of lichen planus.
【學(xué)位授予單位】：吉林大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2017
【分類號(hào)】：R758.65

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