發(fā)布時(shí)間：2018-01-01 10:12

  本文關(guān)鍵詞：中藥成分綠原酸和異綠原酸的敏化熒光與分析方法研究　出處：《河北師范大學(xué)》2017年碩士論文　論文類型：學(xué)位論文

  更多相關(guān)文章： 綠原酸 異綠原酸A 異綠原酸B 異綠原酸C 中藥 菊花 金銀花 熒光分析法 三維熒光二階校正法 薄層吸光掃描法

【摘要】：綠原酸和異綠原酸是重要的中藥活性成分,廣泛存在于藥用植物中,具有多種藥理作用。測(cè)定中藥樣品中綠原酸和異綠原酸的含量對(duì)于中藥質(zhì)量評(píng)價(jià)具有重要意義。本文研究了綠原酸和三種異綠原酸(包括異綠原酸A、異綠原酸B和異綠原酸C)的敏化熒光性質(zhì),進(jìn)而研究了菊花和金銀花中綠原酸和異綠原酸的熒光分析方法,并用薄層吸光掃描法分別測(cè)定了金銀花中綠原酸、異綠原酸A和異綠原酸C的含量。論文包括以下三部分:1.研究了綠原酸和三種異綠原酸的熒光性質(zhì)。綠原酸和異綠原酸水溶液的內(nèi)源熒光均很弱,加入硼砂和溴化十六烷基三甲胺(CTAB)可產(chǎn)生較強(qiáng)的敏化熒光。綠原酸熒光峰的最大激發(fā)/發(fā)射波長(zhǎng)位于350 nm/460 nm,異綠原酸A在350 nm/480 nm,異綠原酸B在350 nm/471nm,異綠原酸C在350 nm/480 nm。四種物質(zhì)的敏化熒光強(qiáng)度均與水溶液中甲醇的體積分?jǐn)?shù)、離子強(qiáng)度、硼砂和CTAB的濃度有關(guān)。在最佳實(shí)驗(yàn)條件下,測(cè)得綠原酸的熒光量子產(chǎn)率為0.0177,異綠原酸A為0.0167,異綠原酸B為0.0154,異綠原酸C為0.0248。2.由于綠原酸和異綠原酸的熒光性質(zhì)非常相似,在不經(jīng)分離的情況下難以測(cè)定各種組分含量,因而用熒光分光光度法和三維熒光二階校正法測(cè)定了菊花和金銀花中綠原酸和異綠原酸的總量。兩種方法對(duì)菊花的測(cè)定結(jié)果的平均值分別為5.59%和5.40%,對(duì)金銀花測(cè)定結(jié)果的平均值分別為7.05%和8.48%。研究結(jié)果表明,熒光分析法可以測(cè)定菊花和金銀花中綠原酸和異綠原酸的總量,進(jìn)而可用于評(píng)估藥材的質(zhì)量。3.用薄層吸光掃描法測(cè)定了金銀花中綠原酸、異綠原酸A和異綠原酸C的含量,結(jié)果分別為2.76%、1.35%和0.733%。實(shí)驗(yàn)表明,金銀花中各組分在硅膠板上能夠完全分離,各組分在薄層板上性質(zhì)穩(wěn)定,測(cè)定精密度好,方法簡(jiǎn)單易行。
[Abstract]:Lv Yuan acid and isob_person1# acid are important active components of traditional Chinese medicine. They are widely used in medicinal plants. Determination of Lv Yuan acid and isob_person1# acid in Chinese traditional medicine samples is of great significance for the quality evaluation of traditional Chinese medicine. The sensitized fluorescence properties of iso#china_person0# acid B and isob_person1# acid C) were studied, and then the fluorescence analysis method of Lv Yuan acid and isob_person2# acid in chrysanthemum and honeysuckle flower was studied, and the Lv Yuan acid in honeysuckle flower was determined by thin layer absorption-scanning method. The contents of iso#china_person0# acid A and isob_person1# acid C. The paper includes three parts: 1. The fluorescence properties of Lv Yuan acid and three kinds of isob_person2# acid were studied. The endogenous fluorescence of Lv Yuan acid and iso#china1# acid aqueous solution was very weak. Addition of borax and cetyltrimethylammonium bromide (CTAB) can produce strong sensitized fluorescence. The maximum excitation / emission wavelength of the fluorescence peak of Lv Yuan is at 350 nm/460 nm. Iso#china_person0# acid A was at 350 nm/480 / nm, and iso#china_person1# acid B was at 350 nm/471nm. The sensitized fluorescence intensity of iso#china_person0# C at 350 nm/480 Nm. The sensitized fluorescence intensity of the four substances was in agreement with the volume fraction and ionic strength of methanol in aqueous solution. Under the optimum experimental conditions, the fluorescence quantum yield of Lv Yuan acid was 0.0177, the isob_person1# acid A was 0.0167, and the iso#china_person2# acid B was 0.0154. Iso#china_person0# acid C is 0.0248.2. Due to the very similar fluorescence properties of Lv Yuan acid and isob_person2# acid, it is difficult to determine the contents of various components without separation. Therefore, the total amount of Lv Yuan acid and isob_person1# acid in chrysanthemum and honeysuckle were determined by fluorescence spectrophotometry and three dimensional fluorescence second order correction method. The average values of the two methods for determination of chrysanthemum were 5.59% and 5.59%, respectively. 0%. The average values of the determination results of honeysuckle were 7.05% and 8.48.The results showed that the total amount of Lv Yuan acid and isob_person1# acid in chrysanthemum and honeysuckle flower could be determined by fluorescence analysis. Then it can be used to evaluate the quality of medicinal materials .3.The content of Lv Yuan acid, isobali_person1# acid A and iso#china_person2# acid C in Flos Lonicerae was determined by thin layer absorption-scanning method. The results were 2.76%, respectively. The experimental results show that the components of Flos Lonicerae can be completely separated on silica gel plate, the properties of each component are stable on the thin layer plate, the precision is good, and the method is simple and feasible.
【學(xué)位授予單位】：河北師范大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2017
【分類號(hào)】：R284.1;O657.3

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