本文選題：荒漠 + 多功能植物根際促生菌��； 參考：《內(nèi)蒙古農(nóng)業(yè)大學(xué)》2017年碩士論文

【摘要】：植物根際促生細(xì)菌(Plant Groeth Promoting Rhizobacteria,PGPR)是植物根際存在的一類能夠促進(jìn)和保護(hù)植物生長(zhǎng)發(fā)育的有益細(xì)菌,可以促使荒漠植物適應(yīng)其所在惡劣生態(tài)環(huán)境,并具有應(yīng)用于生態(tài)農(nóng)業(yè)的潛力。然而,有關(guān)荒漠植物根際土中的PGPR研究還較少,相關(guān)類群組成和功能特性還不清楚。本研究采集了生長(zhǎng)于內(nèi)蒙古巴丹吉林、吉蘭泰、騰格里沙漠和巴拉貢等荒漠中的梭梭(Haloxylon ammodendron)、沙冬青(Ammopiptarthus mongolicus)、白刺(Nitraria tangutorum)、鹽爪爪(Kalidiumm foliatum)、紅砂(Reaumuria soongoricaa)等幾種常見植物的根際土混合樣,應(yīng)用傳統(tǒng)涂布劃線方法,分離篩選了具有固氮、溶磷、產(chǎn)鐵載體、產(chǎn)IAA及產(chǎn)ACC脫氨酶功能的根際細(xì)菌,基于16SrDNA基因序列技術(shù)進(jìn)行了初步分類鑒定。結(jié)果表明,共獲得472株P(guān)GPR,其中用Ashby培養(yǎng)基篩選得到固氮菌有191株,優(yōu)勢(shì)菌門為變形菌門(Proteobacteria)占85.8%,用AZO培養(yǎng)基篩選得到固氮菌149株,優(yōu)勢(shì)菌門為放線菌門(Actinobacteria)占43.7%,用有機(jī)磷培養(yǎng)基篩選得到溶有機(jī)磷菌株53株,優(yōu)勢(shì)菌門為放線菌門(Actinobacteria)占69.8%,溶用NBRIP培養(yǎng)基篩選得到無機(jī)磷菌株79株,優(yōu)勢(shì)菌門為放線菌門(Actinobacteria).占59.5%。將得到的菌株進(jìn)行促生能力分析,最終具有固氮能力,溶磷能力,產(chǎn)鐵載體,產(chǎn)IAA及產(chǎn)ACC脫氨酶能力的細(xì)菌有9株,編號(hào)分別為1、58、B58、B70、界64、Y16-1、Y18、Y34、Y43,經(jīng)PCR擴(kuò)增16SrDN貼,測(cè)定其基因序列與EZ-BioCloud數(shù)據(jù)庫中同源序列進(jìn)行比對(duì),同源性分別與ArthrobacternitroguajacolicusG2-1T、PseudomonasbrassicacearumATCC 49054T、Rhizobium radiobacter ATCC 19358TT、Pantoea conspicua LMG 24534T、Rhodococcus marinonascens DSM 43752T、Gordonia terraeNBRC 100016T、Arthrobbacter nicotianae DSM 20123T、Bacillus cereus ATCC 14579T、Gordonia effusa IFM 10200T最為接近,在99-100%之間。
[Abstract]:Plant Groeth promoting bacteria (PGPR) is a kind of beneficial bacteria which can promote and protect the growth and development of plants in the rhizosphere of plants. It can promote desert plants to adapt to its harsh ecological environment and have the potential to be applied to ecological agriculture. However, there are few studies on PGPR in the rhizosphere of desert plants, and the composition and functional characteristics of related groups are not clear. In this study, the rhizosphere soil mixed samples of Haloxylon ammodendron), (Ammopiptarthus mongolicus), (Ammopiptarthus mongolicus),) grown in Badain Jilin, Jilantai, Tenggari and Barragon deserts in Inner Mongolia were collected, such as Kalidiumm foliatum), red sand (Reaumuria soongoricaa) and so on. Rhizosphere bacteria with nitrogen-fixing, phosphorus-soluble, iron-producing carrier, IAA production and ACC deaminase production were isolated and screened by traditional coating method. The bacteria were preliminarily classified and identified based on 16s rDNA gene sequence technique. The results showed that 472 strains of PGPRs were obtained, of which 191 strains were screened by Ashby medium, 85.8% by Proteobacteria, 149 strains were screened by AZO medium. The dominant phylum was actinomycetes (Actinobacteria) accounting for 43.7%. 53 strains of organophosphorus strains were screened by organic phosphorus medium, 69.8% of them by (Actinobacteria), 79 strains of inorganic phosphorus strains were screened by using NBRIP medium, and 79 strains were screened as (Actinobacteria). Of actinomycetes by using organophosphorus medium. 59.5%. 9 strains of bacteria with nitrogen fixation ability, phosphorus solubilization ability, iron production carrier, IAA production ability and ACC deaminase production capacity were identified as 1t58.B58B70, and 64FY16-1OY18OY34-Y43, respectively, and 16SrDN stickers were amplified by PCR, and the results showed that the bacteria had the ability to fix nitrogen, dissolve phosphorus, produce IAA, and produce ACC deaminase, respectively. 嫻嬪畾鍏跺熀鍥犲簭鍒椾笌EZ-BioCloud鏁版嵁搴撲腑鍚屾簮搴忓垪榪涜姣斿,鍚屾簮鎬у垎鍒笌ArthrobacternitroguajacolicusG2-1T,PseudomonasbrassicacearumATCC 49054T,Rhizobium radiobacter ATCC 19358TT,Pantoea conspicua LMG 24534T,Rhodococcus marinonascens DSM 43752T,Gordonia terraeNBRC 100016T,Arthrobbacter nicotianae DSM 20123T,Bacillus cereus ATCC 14579T,Gordonia effusa IFM 10200T鏈，

本文編號(hào)：2096112