本文選題：不動(dòng)桿菌 + 柴油��； 參考：《中國環(huán)境科學(xué)》2017年11期

【摘要】：在遼河口濕地油田石油污染土壤中分離到一株耐鹽柴油高效降解菌株L7,經(jīng)過形態(tài)學(xué)觀察、16S r RNA基因序列分析鑒定菌株L7屬于不動(dòng)桿菌屬(Acinetobacter).該菌株能夠以柴油、十五烷、十六烷、十七烷、十八烷、十九烷、二十烷和二十二烷為唯一碳源和能源生長(zhǎng).在柴油無機(jī)鹽培養(yǎng)基中,菌株的最適生長(zhǎng)溫度為30°C,最適pH值為7.0,菌株L7在pH 6.0~9.0,鹽度范圍為3%以內(nèi)都可以生長(zhǎng).與其他的菌株相比較,菌株L7在柴油中生長(zhǎng)更加迅速,在最適培養(yǎng)條件下培養(yǎng)4d后菌株的OD600nm就可以達(dá)到4.0,通過紫外分光光度法測(cè)定培養(yǎng)5d后,柴油的降解效率為61.5%.通過對(duì)菌株L7的全基因組測(cè)序和分析,在基因組上找到一個(gè)烷烴羥化酶基因alk B,將基因克隆到p ME6032質(zhì)粒上,并電轉(zhuǎn)至不依賴柴油生長(zhǎng)的惡臭假單胞菌KT2440中,含有該質(zhì)粒的KT2440菌株能夠在柴油無機(jī)鹽培養(yǎng)基中生長(zhǎng).GC-MS檢測(cè)確定了菌株L7及其alk B基因?qū)︼柡屯闊N的降解能力.推測(cè)菌株L7中烷烴降解途徑為末端氧化途徑,是由烷烴羥化酶催化反應(yīng)生成相應(yīng)的醇,最終通過β-氧化反應(yīng)實(shí)現(xiàn)完全礦化.
[Abstract]:A strain L7 was isolated from oil-contaminated soil of Liaohekou Wetland Oilfield. The strain L7 was identified as Acinetobacter by morphological observation and sequence analysis of 16s r RNA gene. The strain can grow with diesel, pentadecane, hexadecane, heptadecane, octadecane, eicosane and 22 alkane as the sole carbon source and energy source. In diesel-inorganic salt medium, the optimum growth temperature was 30 擄C, the optimum pH value was 7.0, the strain L7 could grow within 3% salinity and pH 6.0. Compared with other strains, strain L7 grew more rapidly in diesel oil. The OD600nm of strain L7 could reach 4.0 after 4 d culture under the optimum culture conditions. The degradation efficiency of diesel oil was 61.5% after 5 days of cultivation by UV spectrophotometry. By sequencing and analyzing the whole genome of strain L7, an alkane hydroxylase gene alk B was found in the genome. The gene was cloned into p ME6032 plasmid and transferred to KT2440 of Pseudomonas oxysporum that did not depend on diesel oil growth. The KT2440 strain containing the plasmid was able to grow in diesel inorganic salt medium. GC-MS analysis confirmed the ability of the strain L7 and its alk B gene to degrade saturated alkanes. It is inferred that the degradation pathway of alkanes in strain L7 is the terminal oxidation pathway, which is catalyzed by alkane hydroxylase to produce corresponding alcohols, and finally realizes the complete mineralization by 尾 -oxidation reaction.
【作者單位】： 中國海洋大學(xué)環(huán)境科學(xué)與工程學(xué)院海洋環(huán)境與生態(tài)教育部重點(diǎn)實(shí)驗(yàn)室;青島農(nóng)業(yè)大學(xué)生命科學(xué)學(xué)院;
【基金】：國家水體污染控制與治理科技重大專項(xiàng)(2013ZX07202-007)
【分類號(hào)】：X172

【相似文獻(xiàn)】

相關(guān)期刊論文 前10條

1 張曉霞;王直強(qiáng);李世貴;顧金剛;姜瑞波;;脂肪酸組分分析在不動(dòng)桿菌鑒定中的應(yīng)用[J];生物技術(shù)通報(bào);2009年06期

2 劉子驥;王焱;岳啟安;閻小芹;趙乃昕;徐煥州;咸慶杰;;新型洛菲不動(dòng)桿菌的分離與鑒定[J];中國病原生物學(xué)雜志;2011年07期

3 潘洪濤,劉偉林,孫喜東,蔡穎華,趙金紅;不動(dòng)桿菌的分離鑒定及各類標(biāo)本中的分布[J];微生物學(xué)雜志;1999年03期

4 于秀娟;姚蘋;孔慶蓮;;高耐藥洛菲不動(dòng)桿菌分離株超廣譜β內(nèi)酰胺酶的基因變異[J];山東大學(xué)學(xué)報(bào)(醫(yī)學(xué)版);2007年04期

5 李晴;邵宗澤;;不動(dòng)桿菌生物乳化劑活性成分研究[J];應(yīng)用與環(huán)境生物學(xué)報(bào);2008年04期

6 江寧;余茂R，

本文編號(hào)：1978711