本文選題：凡納濱對(duì)蝦 + 鄰苯二甲酸二甲酯��； 參考：《江蘇農(nóng)業(yè)科學(xué)》2017年17期

【摘要】：以凡納濱對(duì)蝦為試驗(yàn)對(duì)蝦,通過(guò)不同濃度0、2、10、20μg/L鄰苯二甲酸二甲酯(DMP)暴露不同時(shí)間(120、240 h)后,采用酶促反應(yīng)動(dòng)力學(xué)法及實(shí)時(shí)PCR檢測(cè)分析對(duì)蝦殼膜幾丁質(zhì)酶比活力及幾丁質(zhì)酶mRNA表達(dá)量差異。結(jié)果顯示,DMP暴露對(duì)對(duì)蝦殼膜幾丁質(zhì)酶比活力具有抑制作用,但不具有顯著性。不同濃度DMP暴露不同時(shí)間后,對(duì)對(duì)蝦殼膜幾丁質(zhì)酶系列基因的mRNA表達(dá)量均受到不同程度的影響,2μg/L DMP暴露120 h時(shí),對(duì)蝦殼膜Lv CHT3mRNA表達(dá)量顯著上升;2μg/L DMP暴露240 h時(shí),Lv CHT2 mRNA表達(dá)量顯著下降;20μg/L DMP暴露240 h時(shí),Lv CHT6 mRNA表達(dá)量也顯著下降,說(shuō)明DMP對(duì)對(duì)蝦生長(zhǎng)發(fā)育具有毒性。
[Abstract]:Using Penaeus vannamei as an experimental shrimp, after being exposed to DMP20 渭 g / L of dimethyl phthalate for 120240 h at different concentrations, The specific activity of chitinase and the difference of chitinase mRNA expression in shrimp shell membrane were analyzed by enzymatic kinetic method and real-time PCR detection. The results showed that the specific activity of chitinase in shrimp shell membrane was inhibited by DMP exposure, but it was not significant. After exposure to different concentrations of DMP for different time, the mRNA expression of chitinase genes in shrimp shell was affected by different degrees of exposure to 2 渭 g / L DMP for 120h. The expression of Lv CHT3mRNA in shrimp shell membrane was significantly increased with 2 渭 g / L DMP exposure for 240h. The expression of Lv CHT2 mRNA in shrimp shell membrane decreased significantly when exposed to 20 渭 g / L DMP for 240h, indicating that DMP is toxic to the growth and development of Penaeus penaeus.
【作者單位】： 泉州師范學(xué)院化工與材料學(xué)院;
【基金】：國(guó)家自然科學(xué)基金青年科學(xué)基金(編號(hào):31302190) 福建省自然科學(xué)基金(編號(hào):2012J01047) 福建省泉州市優(yōu)秀人才培養(yǎng)專項(xiàng)(編號(hào):16D04)
【分類號(hào)】：S917.4;X503.22

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