光透法定量粗糙裂隙生物堵塞下的隙寬
發(fā)布時(shí)間:2018-02-08 13:23
本文關(guān)鍵詞: 裂隙 生物堵塞 隙寬 光透法 出處:《水文地質(zhì)工程地質(zhì)》2017年06期 論文類型:期刊論文
【摘要】:基于光透法定量測(cè)量裂隙隙寬的原理及在兩相流中的應(yīng)用,設(shè)計(jì)了一組二維粗糙裂隙模擬裝置研究微生物在裂隙地下水系統(tǒng)中的運(yùn)移行為,首次定量研究了生物堵塞過程中隙寬及分布的變化。研究結(jié)果表明:微生物大多以離散菌簇狀形態(tài)存在,未能形成明顯的連續(xù)生物膜,不同空間位置微生物分布具有一定的隨機(jī)性,本試驗(yàn)中微生物接種和營(yíng)養(yǎng)注入方式?jīng)Q定了微生物集中分布在裂隙底部區(qū)域;微生物生長(zhǎng)造成裂隙平均隙寬的下降(從1 181μm降低為1 086μm,下降8.04%),此外,基于光透法測(cè)量的不同空間位置隙寬(除2個(gè)點(diǎn)外)在微生物生長(zhǎng)過程中都有所下降,兩次微生物接種后點(diǎn)2處隙寬下降最明顯(從1 275μm減少到1 081μm),下降了194μm,約為15.22%。光透法作為一種高效無損的室內(nèi)監(jiān)測(cè)手段,將其應(yīng)用于裂隙介質(zhì)的生物堵塞尤其是定量計(jì)算生物膜厚度的研究中,對(duì)裂隙中生物堵塞研究具有重要的參考意義。
[Abstract]:Based on the principle of measuring fissure gap width by photo-permeation method and its application in two-phase flow, a set of two-dimensional rough fracture simulator was designed to study the migration behavior of microorganisms in fractured groundwater system. The changes of gap width and distribution in the process of biological blockage were quantitatively studied for the first time. The results showed that most of the microorganisms existed in the form of discrete bacteria clusters and could not form obvious continuous biofilms. The distribution of microorganism in different space is random. In this experiment, the way of inoculation and nutrition injection determines the distribution of microorganism in the bottom of fissure. The average gap width (from 1 181 渭 m to 1 086 渭 m) was decreased by 8. 04 渭 m. In addition, the gap width at different locations (except 2 points) measured by optical transmission method decreased during the growth process of microorganism, and the mean gap width decreased from 1 181 渭 m to 1 086 渭 m, and decreased by 8. 04 渭 m from 1 181 渭 m to 1 086 渭 m. The gap width at the two points after inoculation was the most obvious (from 1 275 渭 m to 1 081 渭 m, 194 渭 m, about 15.22). Its application in the study of biological blockage in fractured media, especially in the quantitative calculation of biofilm thickness, has an important reference significance for the study of biofilm in fissures.
【作者單位】: 南京大學(xué)地球科學(xué)與工程學(xué)院/表生地球化學(xué)教育部重點(diǎn)實(shí)驗(yàn)室;東華理工大學(xué)水資源與環(huán)境工程學(xué)院;
【基金】:國(guó)家自然科學(xué)基金項(xiàng)目(41472212,41602258)
【分類號(hào)】:X523
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本文編號(hào):1495542
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