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生物炭基固定化菌劑對(duì)石油類(lèi)污染物的高效降解

發(fā)布時(shí)間:2018-01-16 11:57

  本文關(guān)鍵詞:生物炭基固定化菌劑對(duì)石油類(lèi)污染物的高效降解 出處:《環(huán)境工程學(xué)報(bào)》2017年11期  論文類(lèi)型:期刊論文


  更多相關(guān)文章: 生物炭 固定化菌劑 除油率 結(jié)構(gòu) 固定化條件


【摘要】:以生物炭為載體,采用吸附法制備固定化菌劑,通過(guò)分析不同材料生物炭的結(jié)構(gòu)差異,探討了不同生物炭的結(jié)構(gòu)在固定化中的影響機(jī)制。SEM和EDS分析表明,降解菌主要固定在生物炭表面。生物炭?jī)?nèi)含有C、N和P成分,能為降解菌提供營(yíng)養(yǎng)物質(zhì)。生物炭的多孔結(jié)構(gòu)能促進(jìn)石油污染物的降解。結(jié)果表明,經(jīng)玉米芯和秸稈生物炭固定化后F-3、R-7及其混合菌的除油率顯著提高,分別為41.7%和29.5%、52.5%和42.8%、63.8%和53.2%。玉米芯生物炭固定化菌劑的除油率比秸稈生物炭固定化菌劑高10.6%。玉米芯生物炭表面比秸稈生物炭粗糙,其固定的微生物量為4.2×1010cfu·g-1,固定效率達(dá)71.2%;秸稈生物炭固定的微生物量為2.5×1010cfu·g-1,固定效率為57.6%。固定化菌劑的最佳制備條件為:選擇500℃下熱解3 h的玉米芯生物炭為載體,微生物接種量為10%,載體投加量為10 g·L-1,置于35℃、130r·min-1搖床中固定18 h。
[Abstract]:The immobilized microbial agent was prepared by adsorption method with biochar as carrier. The structural differences of biochar with different materials were analyzed. The influence mechanism of the structure of different biochar on immobilization was discussed. SEM and EDS analysis showed that the biodegradable bacteria were mainly fixed on the surface of biochar, and the biochar contained Con N and P components. The porous structure of biochar can promote the degradation of petroleum pollutants. The results showed that F-3 was immobilized with corncob and straw biochar. The oil removal rates of R-7 and its mixed bacteria were 41.7% and 29.52.5% and 42.8%, respectively. The oil removal rate of corn cob biochar was 10.6 higher than that of straw biochar, and the surface of corncob was rougher than that of straw biochar. The fixed microbial biomass was 4.2 脳 10 ~ (10) CFU 路g ~ (-1), and the fixation efficiency was 71.2%. The microbial biomass fixed by straw biochar was 2.5 脳 10 10 CFU 路g -1. The immobilization efficiency was 57.6. The optimum preparation conditions of immobilized bacteria were as follows: the corn cob biochar was pyrolyzed at 500 鈩,

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