本文選題：應(yīng)激　切入點(diǎn)：亞急性酒精中毒模型　出處：《皖南醫(yī)學(xué)院》2014年碩士論文

【摘要】：目的：觀察納洛酮對(duì)應(yīng)激狀態(tài)下亞急性酒精中毒大鼠模型影響大鼠學(xué)習(xí)記憶功能的干預(yù)作用。 方法：62只雄性SD大鼠隨機(jī)分為6組：空白對(duì)照組（C, n=11），生理鹽水組（NS，n=6），納洛酮組（N，n=11），應(yīng)激組（St，n=11），亞急性酒精中毒組（SA，n=11），應(yīng)激后亞急性酒精中毒組（St+SA，n=12）。適應(yīng)性飼養(yǎng)后開始建立模型，將St組和St+SA組大鼠放入電擊箱適應(yīng)1天后開始給予“聲音-電擊”配對(duì)刺激，連續(xù)3天，第5天將大鼠放入電擊箱不給予任何刺激，只檢測(cè)大鼠的木僵反應(yīng)時(shí)間；應(yīng)激后給予SA組和St+SA組大鼠進(jìn)行酒精灌胃10天，劑量為15ml/kg，灌胃酒精第1天給予5%濃度酒精溶液，之后在此基礎(chǔ)上每天增加5%濃度，第7天后酒精濃度保持為35%；以同樣的劑量給予NS組大鼠進(jìn)行生理鹽水灌胃。使用恐懼監(jiān)測(cè)系統(tǒng)檢測(cè)大鼠的應(yīng)激狀態(tài)，使用曠場(chǎng)試驗(yàn)檢測(cè)大鼠攝入酒精前后的活動(dòng)度和運(yùn)動(dòng)功能的協(xié)調(diào)度，并使用Morris水迷宮檢測(cè)納洛酮對(duì)應(yīng)激狀態(tài)下亞急性酒精中毒模型大鼠影響學(xué)習(xí)記憶功能的干預(yù)作用。 結(jié)果： 1.恐懼監(jiān)測(cè)結(jié)果分析 應(yīng)激前后、酒精灌胃前后和納洛酮干預(yù)后各組大鼠恐懼檢測(cè)數(shù)據(jù)顯示，測(cè)試日間F（3，168）=12.53，P0.001，處理組間F（5，56）=13.60，P0.001； SNK-q兩兩比較：St組和St+SA組與其他四組之間的恐懼監(jiān)測(cè)都存在著顯著性差異（P0.05）。首先，應(yīng)激前后6組大鼠恐懼監(jiān)測(cè)數(shù)據(jù)的比較：St組（t=-8.28，P0.001）與St+SA組（t=-6.62，P0.001）都具有顯著性差異。應(yīng)激后C組與St組（t=-7.52，P0.001）、C組與St+SA組（t=-5.19，，P0.001）、SA組與St+SA組之間也具有顯著性差異（t=-2.43，P0.05）。其次，酒精灌胃前后6組大鼠恐懼監(jiān)測(cè)數(shù)據(jù)的比較：N組（t=-3.89，P0.001）、St組（t=4.48，P0.001）、St+SA組（t=2.27，P0.05）具有顯著性差異；C組與NS組大鼠在酒精灌胃前（t=-0.12，P0.05）和酒精灌胃后（t=-0.56，P0.05）均無顯著性差異。最后，納洛酮干預(yù)后6組大鼠恐懼監(jiān)測(cè)數(shù)據(jù)相比F=3.50，P0.01；N組、St組和St+SA組分別與其他組相比P0.05。 2.曠場(chǎng)試驗(yàn)結(jié)果分析 6組大鼠酒精灌胃后的曠場(chǎng)試驗(yàn)中央格停留時(shí)間（F=2.36，P0.05）、總穿格數(shù)（F=7.04，P0.001）、站立次數(shù)（F=8.56，P0.001）都具有顯著性差異，修飾次數(shù)和糞便粒數(shù)（P0.05）無顯著性差異；采用SNK-q兩兩比較，總穿格數(shù)C組與其他5組之間P0.05， St組與其他4組（不包括N組）之間P0.05，SA組與其他5組之間P0.05，St+SA組與其他5組之間P0.05；站立次數(shù)C組和St組分別與SA組和St+SA組之間P0.05。6組大鼠酒精灌胃前后的曠場(chǎng)試驗(yàn)中總穿格數(shù)（t=9.57，P0.001）、站立次數(shù)（t=6.85，P0.001）、修飾次數(shù)（t=-4.93，P0.001）、糞便粒數(shù)（t=2.10，P0.05）都具有顯著性差異。C組與SA組大鼠在酒精灌胃前后曠場(chǎng)試驗(yàn)的中央格停留時(shí)間（t=3.31，P0.01）、總穿格數(shù)（t=2.60，P0.05）和站立次數(shù)（t=3.49，P0.01）均具有顯著性差異。 3. Morris水迷宮測(cè)試結(jié)果分析 采用雙因素方差分析（重復(fù)測(cè)量）對(duì)大鼠行為Morris水迷宮訓(xùn)練定位航行階段7天的逃避潛伏期進(jìn)行分析，測(cè)試日間F（6，336）=23.15，P0.001；處理組間F（5，56）=1.34，P0.05；測(cè)試日間與組別的交互作用F（1，56）=594.84，P0.001；且NS組與St+SA組之間差異有統(tǒng)計(jì)學(xué)意義（P0.05）。采用單因素方差分析對(duì)每組大鼠7天的行為Morris水迷宮訓(xùn)練定位航行階段的逃避潛伏期進(jìn)行分析，C組（F=6.19，P0.001），NS組（F=4.77，P0.001），N組（F=6.96，P0.001），St組（F=4.77，P0.001），SA組（F=1.14，P0.05），St+SA組（F=3.32，P0.01）。定位航行階段第4天C組與SA組大鼠的逃避潛伏期之間具有顯著性差異（t=2.32，P0.05）。 通過卡方檢驗(yàn)對(duì)大鼠7天定位航行階段的分析策略構(gòu)成比進(jìn)行分析，Day1(χ2=867.20，P0.001)，Day2(χ2=898.70，P0.001)，Day3(χ2=666.30，P0.001)，Day4(χ2=825.70，P0.001)，Day5(χ2=108.90，P0.001)，Day6(χ2=932.80，P0.001)，Day7(χ2=675.40，P0.001)。 采用雙因素方差分析（重復(fù)測(cè)量）對(duì)大鼠行為Morris水迷宮訓(xùn)練定位航行階段7天的游泳速度進(jìn)行分析，測(cè)試日間（F=19.19，P0.001），在定位航行階段第5天處理組間（F=3.25，P0.01），且在整個(gè)定位航行階段中各組的游泳速度呈下降趨勢(shì)；第6天C組與SA組之間（F=-2.68，P0.05），第5天C組與St+SA組具有顯著性差異（F=2.45，P0.05）。 比較大鼠在定位航行階段的靶象限活動(dòng)時(shí)間百分比，第4天C組分別與SA組（t=2.13，P0.05）、St+SA組（t=2.18，P0.05）之間差異具有統(tǒng)計(jì)學(xué)意義；第6天NS組與N組之間差異顯著（t=-2.25，P0.05）。 NS組與N組大鼠在空間探索階段的穿臺(tái)次數(shù)差異具有統(tǒng)計(jì)學(xué)意義（t=-2.34，P0.05）。 4.各組大鼠體重比較 采用雙因素方差分析（重復(fù)測(cè)量）對(duì)各組大鼠在應(yīng)激前后、酒精灌胃前后和納洛酮干預(yù)后的體重進(jìn)行分析，與C組相比，模型組大鼠的體重增長明顯受抑制（P0.05），并且各處理組間相比F（5，56）=11.71，P0.001；測(cè)試日間F（4，224）=213.80，P0.001。使用SNK-q進(jìn)行兩兩比較， SA組和St+SA組與其他組相比P0.05，而SA組和St+SA組等之間的體重?zé)o顯著性差異（P0.05）。 結(jié)論酒精對(duì)大鼠的活動(dòng)度和體重增長等具有抑制作用；應(yīng)激狀態(tài)下亞急性酒精中毒可損害大鼠學(xué)習(xí)記憶功能；納洛酮對(duì)應(yīng)激狀態(tài)下亞急性酒精中毒大鼠學(xué)習(xí)記憶功能有一定的改善作用。
[Abstract]:Objective : To observe the effect of naloxone on learning and memory function in rats with subacute alcoholism .

Methods : 62 male SD rats were randomly divided into six groups : blank control group ( C , n = 11 ) , physiological saline group ( NS , n = 6 ) , naloxone group ( N , n = 11 ) , stress group ( St , n = 11 ) , subacute alcoholism group ( SA , n = 11 ) , post - stress subacute alcoholism group ( St + SA , n = 12 ) . After adaptive feeding , the model was established , and the rats in the St and St + SA groups were put into the electric shock box for 1 day , then the " voice - electric shock " pairing was started , and the rats were put into the electric shock box for 3 days , and the rats were put into the electric shock box without any stimulation , and the reaction time of the rats was detected only ;
After stress , the rats were given SA group and St + SA group for 10 days , the dosage was 15ml / kg , the alcohol solution of 5 % concentration was given on the first day of alcohol administration , then the concentration of alcohol was increased by 5 % and the alcohol concentration was kept at 35 % after 7 days .
In the same dose , NS group rats were given normal saline enema . Using the fear monitoring system to detect the stress state of rats , the activity degree and the coordination degree of motion function before and after alcohol intake were detected by open field test , and the effect of naloxone on learning and memory function was studied by Morris water maze test in rats with subacute alcoholism model .

Results :

1 . Analysis of the results of fear monitoring

The results showed that F ( 3,168 ) = 12.53 , P0.001 , F ( 5,56 ) = 13.60 , P0.001 ;
Compared with the other four groups ( t = - 8.28 , P0.001 ) and St + SA group ( t = - 6.62 , P0.001 ) , there was significant difference between group C and St group ( t = - 7.52 , P0.001 ) , group C and St + SA group ( t = - 5.19 , P0.001 ) , and there was significant difference between group SA and St + SA group ( t = - 2.43 , P0.05 ) . Secondly , there was significant difference between group N ( t = - 3.89 , P0.001 ) , St group ( t = 4.48 , P0.001 ) , St + SA group ( t = 2.27 , P0.05 ) .
There was no significant difference between group C and NS group ( t = - 0.12 , P0.05 ) and alcohol ( t = - 0.56 , P0.05 ) .
Compared with other groups , the group N , St and St + SA were significantly higher than those in other groups ( P0.05 ) .

2 . Analysis of open field test results

The time ( F = 2.36 , P < 0.01 ) and the number of standing ( F = 8.56 , P0.001 ) were significantly different between the six groups ( F = 2.36 , P < 0.01 ) .
Compared with other five groups , there was no significant difference between the group C and the other 5 groups ( P0.05 ) .
There was significant difference between group C and group SA ( t = 9.57 , P0.001 ) , number of standing ( t = 6.85 , P0.001 ) , number of days ( t = - 4.93 , P0.001 ) , number of feces ( t = - 4.93 , P0.001 ) , number of stool grains ( t = - 4.93 , P0.05 ) , and the number of standing ( t = 2.60 , P0.05 ) and standing frequency ( t = 3.49 , P0.01 ) .

3 . Analysis of Morris water maze test results

Two - factor variance analysis ( repeated measurement ) was used to analyze the escape latency of Morris water maze training for 7 days in Morris water maze training , and the daytime F ( 6,336 ) = 23.15 , P0.001 ;
F ( 5 , 56 ) = 1 . 34 , P 0 . 05 in treatment group ;
F ( 1 , 56 ) = 594.84 , P0.001 ;
In group C ( F = 6.19 , P0.001 ) , NS group ( F = 4.77 , P0.001 ) , NS group ( F = 4.77 , P0.001 ) , group N ( F = 6.96 , P0.001 ) , St group ( F = 4.77 , P0.001 ) , SA group ( F = 1.14 , P0.05 ) , St + SA group ( F = 3.32 , P0.01 ) . There was a significant difference ( t = 2.32 , P < 0.05 ) between group C and SA group ( t = 2.32 , P0.05 ) .

Day1 ( 蠂2 = 867.20 , P0.001 ) , Day2 ( 蠂2 = 898.70 , P0.001 ) , Day3 ( 蠂2 = 867.30 , P0.001 ) , Day5 ( 蠂2 = 108.90 , P0.001 ) , Day6 ( 蠂2 = 932.80 , P0.001 ) , Day7 ( 蠂2 = 670.40 , P0.001 ) .

Two - factor analysis ( repeated measurement ) was used to analyze the swimming speed of rats ' behavior Morris water maze training for 7 days , and the daytime ( F = 19.19 , P0.001 ) was measured , and the swimming speed of each group was decreased during the fifth day of the navigation phase ( F = 3.25 , P0.01 ) .
There was significant difference between group C and SA group ( F = - 2.68 , P0.05 ) .

Compared with SA group ( t = 2.13 , P 0.05 ) and St + SA group ( t = 2.18 , P 0.05 ) , the percentage of active time of target quadrant was compared with that of group SA ( t = 2.13 , P0.05 ) .
There was a significant difference between NS group and N group on Day 6 ( t = - 2.25 , P0.05 ) .

There was significant difference between NS group and N group ( t = - 2.34 , P0.05 ) .

4 . Body weight comparison in each group

Compared with group C , the weight gain of model group rats was significantly inhibited compared with group C ( P0.05 ) , and F ( 5,56 ) = 11.71 , P0.001 ;
Compared with other groups , there was no significant difference between SA group and St + SA group ( P0.05 ) .

Conclusion Alcohol has an inhibitory effect on the activity and body weight gain of rats .
Subacute alcoholism could damage the learning and memory function of rats in stress state .
The effects of naloxone on learning and memory function in rats with subacute alcoholism were improved .

【學(xué)位授予單位】：皖南醫(yī)學(xué)院
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2014
【分類號(hào)】：B842.3

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