本文關(guān)鍵詞：生物表面活性劑對(duì)東海原甲藻生長(zhǎng)的影響，由筆耕文化傳播整理發(fā)布。

中國(guó)環(huán)境科學(xué) 2004,24(6)：692~696 China Environmental Science

生物表面活性劑對(duì)東海原甲藻生長(zhǎng)的影響

2.中國(guó)海洋大學(xué)生命學(xué)院,山東 青島 266003)

龔良玉1,李雁賓2,王修林1＊,梁生康1,祝陳堅(jiān)1,韓秀榮1 (1.中國(guó)海洋大學(xué)化學(xué)化工學(xué)院,山東 青島 266003；

摘要：以東海原甲藻為實(shí)驗(yàn)材料,研究了銅綠假單胞菌產(chǎn)鼠李糖脂類生物表面活性劑對(duì)藻細(xì)胞的抑制和殺藻作用.結(jié)果表明,鼠李糖脂在較低濃度下對(duì)東海原甲藻的生長(zhǎng)有明顯的抑制效果,增大用量,可直接殺滅藻細(xì)胞.生長(zhǎng)延滯期的藻細(xì)胞對(duì)鼠李糖脂的作用更為敏感.在相同實(shí)驗(yàn)條件下,鼠李糖脂對(duì)綠藻生長(zhǎng)的影響基本可以忽略,在低濃度下對(duì)中肋骨條藻和湛江叉鞭金藻生長(zhǎng)的影響也很弱,鼠李糖脂的濃度增至5.0mg/L以上時(shí),對(duì)中肋骨條藻和湛江叉鞭金藻的生長(zhǎng)表現(xiàn)出一定的抑制作用. 關(guān)鍵詞：東海原甲藻；生物表面活性劑；生長(zhǎng)抑制；殺藻

中圖分類號(hào)：X171 文獻(xiàn)標(biāo)識(shí)碼：A 文章編號(hào)：1000－6923(2004)06－0692－05

The influence of biosurfactant on the growth of Prorocentrum donghaiense. GONG Liang-yu1, LI Yan-bin2, WANG Xiu-lin1, LIANG Sheng-kang1, ZHU Chen-jian1, HAN Xiu-rong1 (1.College of Chemistry and Chemical Engineering, Ocean University of China, Qingdao 266003, China；2.College of Marine Life Science, Ocean University of China, Qingdao 266003, China). China Environmental Science, 2004,24(6)：692~696

Abstract：The inhibiting and killing functions of rhamnolipid biosurfactants produced by Pseudomonas aeruginosa on alga cells were studied with Prorocentrum donghaiense as experiment material. The growth of P. donghaiense was inhibited markedly in lower concentration of rhamnolipids, moreover, the alga cell would be killed directly with increasing its using amount. The alga cells in the lag phase of growth were more susceptible to the function of rhamnolipids. Under the same experiment condition, the influence of the rhamnolipids on green algae was negligible basically; at low concentration of the rhamnolipids the influence on S. costatum and D. zhanjiangensis was also very weak; and expressed only definite inhibition action at concentration increased to above 5.0mg/L. Key words：Prorocentrum donghaiense；biosurfactant；growth inhibition；alga-killing

東海原甲藻(Prorocentrum donghaiense)(又稱具齒原甲藻,Prorocentrum dentatum)是長(zhǎng)江口和浙江海域的主要藻類之一[1],近年來(lái)多次引發(fā)大面積赤潮[2], 嚴(yán)重破壞了海洋生態(tài)環(huán)境.

研究表明,表面活性劑容易吸附在具有膜層結(jié)構(gòu)的藻細(xì)胞表面,從而影響和破壞藻細(xì)胞正常的生理功能[3].曹西華等[3]探討了季銨鹽陽(yáng)離子表面活性劑對(duì)赤潮異灣藻的殺滅作用.Ahn等[4]分析了含有生物表面活性劑的細(xì)菌發(fā)酵液對(duì)藍(lán)藻生長(zhǎng)的影響.生物表面活性劑與化學(xué)表面活性劑相比,具有生物可降解性、低毒性和通過(guò)微生物發(fā)酵生產(chǎn)簡(jiǎn)單等優(yōu)點(diǎn),在赤潮治理中具有廣泛的應(yīng)用前景.銅綠假單胞菌培養(yǎng)時(shí)產(chǎn)生的鼠李糖脂類生物表面活性劑,具有較強(qiáng)的抑菌活性[5],但.表面活性劑用于東海原甲藻生長(zhǎng)影響以及殺藻作用的實(shí)驗(yàn)研究,以期為東海原甲藻赤潮的治理提供參考. 1 材料與方法　1.1 實(shí)驗(yàn)材料

實(shí)驗(yàn)藻種為東海原甲藻0201-01[國(guó)家重點(diǎn)基礎(chǔ)研究發(fā)展規(guī)劃項(xiàng)目(2001CB409703)組提供]、亞心形扁藻(Platymonas subcordiformis)、青島大扁藻(Platymonas helgolandica)、鹽生杜氏藻(Dunaliella salina)、湛江叉鞭金藻(Dicrateria

收稿日期：2004－03－29

基金項(xiàng)目：國(guó)家重點(diǎn)基礎(chǔ)研究發(fā)展規(guī)劃項(xiàng)目（２００１ＣＢ４０９７０３，，　２００２Ｃ�。拢罚保矗埃埃福�

* , 教授

  本文關(guān)鍵詞：生物表面活性劑對(duì)東海原甲藻生長(zhǎng)的影響，由筆耕文化傳播整理發(fā)布。