本文關(guān)鍵詞：耐草甘膦轉(zhuǎn)基因大豆對(duì)土壤線(xiàn)蟲(chóng)多樣性的影響，由筆耕文化傳播整理發(fā)布。

耐草甘膦轉(zhuǎn)基因大豆對(duì)土壤線(xiàn)蟲(chóng)多樣性的影響

Effect of Glyphosate-tolerant Soybean on Diversity of Soil Nematodes

[1] [2] [3] [4] [5] [6] [7]

YANG Zhi-guo, ZHAO Jian-ning, LI Gang, XIU Wei-ming, ZHANG Qiu-yan, CHANG Hong, YANG Dian-lin （ 1.College of Life Science, Shanxi Agricultural University, Taigu 030801, China;

[1]山西農(nóng)業(yè)大學(xué)生命科學(xué)學(xué)院,山西太谷030801; [2]農(nóng)業(yè)部環(huán)境保護(hù)科研監(jiān)測(cè)所農(nóng)業(yè)部產(chǎn)地環(huán)境質(zhì)量重點(diǎn)實(shí)驗(yàn)室/天津市農(nóng)業(yè)環(huán)境與農(nóng)產(chǎn)品安全重點(diǎn)實(shí)驗(yàn)室,天津300191

文章摘要：為了評(píng)價(jià)耐草甘膦轉(zhuǎn)基因大豆對(duì)土壤線(xiàn)蟲(chóng)的影響，在網(wǎng)室盆栽試驗(yàn)條件下，研究了成熟期耐草甘膦轉(zhuǎn)基因大豆M88根際土壤線(xiàn)蟲(chóng)豐度、營(yíng)養(yǎng)類(lèi)群組成和生態(tài)學(xué)指數(shù)以及土壤環(huán)境因子和線(xiàn)蟲(chóng)群落之間的相關(guān)性。結(jié)果表明：試驗(yàn)共分離鑒定出23個(gè)土壤線(xiàn)蟲(chóng)屬，8個(gè)功能群，，其中非轉(zhuǎn)基因大豆中黃13（Z13）種植條件下分離鑒定出土壤線(xiàn)蟲(chóng)23屬，耐草甘膦轉(zhuǎn)基因大豆M88種植條件下分離鑒定出土壤線(xiàn)蟲(chóng)21屬；耐草甘膦轉(zhuǎn)基因大豆M88的土壤線(xiàn)蟲(chóng)平均豐度為（1143．42±135．04）·100g^-1干土，顯著高于（P〈O．05）非轉(zhuǎn)基因大豆中黃13[（756．83±141．33）·100g^-1干土】；耐草甘膦轉(zhuǎn)基因大豆M88顯著升高了雜食捕食性線(xiàn)蟲(chóng)擬桑尼屬（Thorneella）的相對(duì)豐度（P〈0．01）。雖然非轉(zhuǎn)基因大豆中黃13種植條件下的自由生活線(xiàn)蟲(chóng)成熟度指數(shù)腳和結(jié)構(gòu)指數(shù)s，顯著低于耐草甘膦轉(zhuǎn)基因大豆M88（P〈0．05），但是兩種大豆種植條件下土壤線(xiàn)蟲(chóng)的Shannon—Wiener多樣性指數(shù)日’、屬豐富度指數(shù)GR和營(yíng)養(yǎng)多樣性指數(shù)TD均無(wú)顯著差異，說(shuō)明耐草甘膦大豆M88對(duì)線(xiàn)蟲(chóng)群落結(jié)構(gòu)多樣性影響不顯著。冗余分析RDA排序圖顯示：線(xiàn)蟲(chóng)功能組Om4和土壤有機(jī)質(zhì)OM呈顯著的正相關(guān)，而與硝態(tài)氮NN呈顯著的負(fù)相關(guān)。

Abstr：Transgenic plants may affect microbial community structure and functions in soil ecosystems. A pot culture experiment was con- ducted to study the responses of soil nematodes to herbicide-tolerant soybean by analyzing the abundance, trophic group composition, eco- logical indices of soil nematodes and the relationship between soil environmental factors and soil nematode communities using non-trans- genic soybean Z13 as the control. Nematodes in the rhizosphere of herbicide-tolerant soybean M88 and non-transgenic soybean Z13 were separated by the Baermann tray method and identified to genus level using an inverted Compound microscope. Twenty-three genera and twenty-one genera were found in the rhizosphere of the Z13 and M88, respectively. The average abundance of nematodes was （ 1 143.42 ± 135.04） individuals per 100 g dry soil for the M88, whereas （756.83 ±141.33 ）individuals per 100 g dry soil for the Z13. They were signif- icantly different（P〈0.05 ）. Compared with the Z13, the M88 significantly increased the relative abundance of Thorneella genus（P〈0.01 ）. Although the maturity index（M1） and structure index（Sl） of free-living nematodes were significantly higher in the M88 than in the Z13, but no significant differences were found in Shannon-Wiener diversity index H＇, Genus richness Index GR and Trophic diversity Index TD be- tween the M88 and Z13. The redundancy analysis（RDA） diagram showed that the functional guilds Om4 had significant positive correlation with organic matter（OM）, but significant negative correlation with nitrate nitrogen（NN）. These results suggest that herbicide-tolerant soy- bean M88 has no significant effects on the diversity of soil nematode community.

文章關(guān)鍵詞：

Keyword：:glyphosate-tolerant soybean soil nematode ecological index redundancy analysis i RDA）

課題項(xiàng)目：基金項(xiàng)目：轉(zhuǎn)基因生物新品種培育重大專(zhuān)項(xiàng)（2013ZX08012-005）;中央級(jí)公益性科研院所基本科研業(yè)務(wù)費(fèi)專(zhuān)項(xiàng)資金（農(nóng)業(yè)部環(huán)境保護(hù)科研監(jiān)測(cè)所）;農(nóng)業(yè)部產(chǎn)地環(huán)境質(zhì)量重點(diǎn)實(shí)驗(yàn)室,天津市農(nóng)業(yè)環(huán)境與農(nóng)產(chǎn)品安全重點(diǎn)實(shí)驗(yàn)室開(kāi)放基金項(xiàng)目

  本文關(guān)鍵詞：耐草甘膦轉(zhuǎn)基因大豆對(duì)土壤線(xiàn)蟲(chóng)多樣性的影響，由筆耕文化傳播整理發(fā)布。