本文關(guān)鍵詞：固碳產(chǎn)油微藻的基因工程改造，由筆耕文化傳播整理發(fā)布。

固碳產(chǎn)油微藻的基因工程改造

Genetic Engineering Modification of Microalgae to Enhance CO2 Fixation and Oil Formation

[1] [2] [3]

YU Shui-yan ZHAO Quan-yu SHI Ji-ping （Shanghai Advanced Research Institute, Chinese Academy of Sciences, Shanghai 201210, China）

中國科學(xué)院上海高等研究院,上海201210

文章摘要：藻種的選育和基因工程改造是微藻生物柴油研究的核心。為此，，簡要綜述了微藻從光合作用到甘油三酯（TAG）合成過程中的關(guān)鍵基因及其代謝調(diào)控等方面的研究進展。從光合作用固碳、中心碳代謝、脂肪酸合成、TAG的組裝、抑制TAG合成的競爭途徑及脂類的分解途徑等幾個方面入手，淺析各個代謝途徑中關(guān)鍵基因的作用及其表達調(diào)控。在此基礎(chǔ)上，探討微藻基因工程改造的可行性并指出微藻生物柴油在生物質(zhì)能源領(lǐng)域中的前景及其綜合利用的發(fā)展優(yōu)勢。

Abstr：Fossil fuels are un-renewable and over-emission of CO2 brings on the global warming. Biodiesel from microalgae is considered as a potential alternative energy. CO2 could be fixed by microalgae via photosynthesis to synthesize the oil. Microalgal biodiesel is further produced by trans-esterification of triglycerides with methanol. There are several advantages of microalgae biodiesel compared with other biofuels. Mie a next generation feedstock for biofuel production have obtained worldwide attention. The neutral lipids roalgae especiallytriacylglycerols （TAG） which are the main feedstock of oil can be accumulated in many microalgae cells under stress conditions. Three promising strategies are feasible to enhance TAG accumulation in microalgae： the biochemical engineering approach, the genetic engineering （GE） approach, and the transcription factor engineering approach The genetic engineering modification has now become a new research hot point to enhance the lipid production in oil-rich microalgae pathway in microalgae, and the metabolic An overview of the advances of some genes in lipid biosynthesis regulation strategies for enhancing lipid accumulation was presented. Correlated with the TAG accumulating, some genes in six metabolic pathways are summarized including carbon fixation, central carbon metabolism, fatty acid biosynthesis, TAG assembling, inhibition of competing pathways of TAG and the lipid degradation catabolism. Exploring these enzymes and their functions involved in the pathways are helpful for the genetic engineering modification of mieroalgae. Finally, the current genetic engineering methods and technical issues microalgae and possibilities of microalgae discussed of microalgae, the feasibility of genetic engineer

文章關(guān)鍵詞：

Keyword：:Algae design Genetic engineering Carbon metabolism Metabolic regulation

課題項目：國家自然科學(xué)基金（31201975）、上海市浦江人才計劃（12PJ1408000）、上海市博士后科研資助計劃（12R214216000）資助項目

作者信息：會員可見

  本文關(guān)鍵詞：固碳產(chǎn)油微藻的基因工程改造，由筆耕文化傳播整理發(fā)布。