鹽分是限制全球水稻產量的主要非生物脅迫因素之一。長期的鹽脅迫會引起水稻植株慢性離子毒害和水分滲透失衡,并導致死亡。水稻是全球三大主要糧食作物之一,因此,研究水稻在不同脅迫水平下耐鹽性的遺傳機制將有助于水稻抗逆育種。本研究,我們調查了超級雜交稻兩優(yōu)培九（LYP9）的親本93-11和Pei-ai64s（PA64s）衍生的132個重組自交系（RIL）的耐鹽性。進行了鹽脅迫下數量性狀基因座（QTL）和全基因組轉錄組分析,以及新主效QTL的精細定位。該研究的結果如下:一.鑒定了38個與鹽脅迫相關的QTL。為了確定水稻鹽分脅迫耐受性的遺傳基礎,在兩種鹽濃度處理下,對6個相關性狀的QTL進行了定位�；�132個RIL的高密度遺傳連鎖圖譜,我們對50和100 mM NaCl脅迫下的6個重要農藝性狀進行了QTL分析,包括莖長（SL）、根長（RL）、莖鮮重（SFW）、根鮮重（RFW）、莖干重量（SDW）和根干重（RDW）。共定位到38個與6個性狀相關的QTL,分布在1、2、3、4、5、6、7和10號染色體上。在2種脅迫水平下,共有19個QTL分布在5個QTL簇中。特別是,在兩種鹽濃度水平下,都檢測到一個控... 

【文章來源】：中國農業(yè)科學院北京市

【文章頁數】：109 頁

【學位級別】：博士

【文章目錄】：
摘要
Abstract
LIST OF ABBREVIATIONS
CHAPTER 1 INTRODUCTION
    1.1 General Introduction
    1.2 Review of literature
        1.2.1 Rice:present status
        1.2.2 Salinity stress effects on rice production
        1.2.3 Salinity stress effects on rice plants
    1.3 Salinity stress sensing in rice plant
    1.4 Response of rice under salinity
        1.4.1 Morpho-physiological response
        1.4.2 Response at the biochemical level
        1.4.3 Response at molecular level
    1.5 Defense system of rice against salinity stress
    1.6 Transcriptional regulation and gene expression of salinity tolerance
    1.7 Molecular genetic mapping analysis of QTLs
    1.8 Genetics and QTL mapping of salinity tolerance
CHAPTER 2 Mapping Quantitative Trait Loci Associated with Salinity Tolerance in RIL Population of Rice(Oryza sativa L.)
    2.1 Introduction
    2.2 Materials and methods
        2.2.1 Plant materials and population development
        2.2.2 Treatment of salt stress
        2.2.3 Phenotypic evaluation of parents and progenies
        2.2.4 Statistical analysis
        2.2.5 Construction of linkage map and QTL analysis
    2.3 Results
        2.3.1 Evaluation of phenotypic characteristics under salt stress
        2.3.2 Correlation analysis of traits
        2.3.3 Identification of QTLs for seedling-stage salt tolerance traits
    2.4 Discussion
    2.5 Conclusion
CHAPTER 3 Comparative Transcriptome Profiling of93-11 and PA64s and Identification of Candidate Genes Involved in Salinity Tolerance
    3.1 Introduction
    3.2 Materials and methods
        3.2.1 Plant growth conditions and salinity stress treatment
        3.2.2 Phenotypic evaluation of seedlings under salt stress treatment
        3.2.3 RNA extraction and c DNA library preparation and sequencing
        3.2.4 Data filtering and assembly
        3.2.5 Analysis and functional annotation of DEGs
        3.2.6 Preparation of RNA
        3.2.7 Synthesis of c DNA
    3.3 Results
        3.3.1 PA64s seedlings are more tolerant to salinity than93-11 seedlings
        3.3.2 m RNA sequencing and functional annotation
        3.3.3 Expression profiling analysis in the PA64s and93-11 under salinity
        3.3.4 Gene ontology(GO)enrichment classification of the DEGs
        3.3.5 Identification of differential transcription factor(TFs)
        3.3.6 KEGG pathway enrichment analysis of the DEGs
        3.3.7 Identification of candidate genes within the stable QTLs via RNA-seq
    3.4 Discussion
    3.5 Conclusion
CHAPTER 4 Genetic Dissection of Major Quantitative Trait Locus q SL7 for Shoot Length under Salinity Stress in Rice(Oryza sativa L.)
    4.1 Introduction
    4.2 Materials and methods
        4.2.1 Plant materials and population development
        4.2.2 Growing conditions
        4.2.3 Phenotypic evaluation of93-11,CSSL-q SL7 and mapping populations
        4.2.4 Na+/K+ion measurement
        4.2.5 Photosynthetic pigment content measurement
        4.2.6 Determination of soluble sugar
        4.2.7 Determination of proline
        4.2.8 DNA isolation and PCR analysis
        4.2.9 Statistical analysis of data
    4.3 Result
        4.3.1 CSSL-q SL7 shows more salt tolerant than93-11
        4.3.2 Photosynthetic capacity in93-11 and CSSL-qSL7
        4.3.3 Accumulation of soluble sugar and proline in93-11 and CSSL-q SL7
        4.3.4 Fine mapping of qSL7
    4.4 Discussion
    4.5 Conclusion
CHAPTER 5 Major findings and future perspectives
    5.1 Major findings
    5.2 Future perspectives
REFERENCES
APPENDIX
LIST OF PUBLICATIONS
ACKNOWLEDGEMENT
AUTHOR'S RESUME


【參考文獻】：
期刊論文
[1]水稻耐鹽基因定位與克隆及品種耐鹽性分子標記輔助選擇改良研究進展[J]. 井文,章文華.  中國水稻科學. 2017(02)
[2]Selection of Rice Genotypes for Salinity Tolerance Through Morpho-Biochemical Assessment[J]. Md. Nasim ALI,Bhaswati GHOSH,Saikat GANTAIT,Somsubhra CHAKRABORTY.  Rice Science. 2014(05)
[3]Dissection of genetic overlap of salt tolerance QTLs at the seedling and tillering stages using backcross introgression lines in rice[J]. ZANG JinPing 1 ,SUN Yong 1 ,WANG Yun 1 ,YANG Jing 1 ,LI Fang 1 ,ZHOU YongLi 1 ,ZHU LingHua 1 , Reys JESSICA2,Fotokian MOHAMMADHOSEIN 2,XU JianLong 1&LI ZhiKang 1,2 1Institute of Crop Sciences/National Key Facility for Crop Gene Resources&Genetic Improvement,Chinese Academy of Agricul- tural Sciences,Beijing 100081,China; 2International Rice Research Institute,DAPO Box 7777,Metro Manila,Philippines.  Science in China（Series C:Life Sciences）. 2008(07)
[4]高質量植物基因組DNA的分離[J]. 羅志勇,周鋼,陳湘暉,陸秋恒,胡維新.  湖南醫(yī)科大學學報. 2001(02)



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