大豆胞囊線蟲�。⊿CN,Heterodera glycines Ichinohe）是世界性的重要病害,每年可導(dǎo)致幾十億美元的經(jīng)濟損失。在大豆胞囊線蟲病的防控策略中,培育抗性品種是行之最為有效的方法。鑒于大豆胞囊線蟲病的危害嚴(yán)重性,開展大豆SCN抗性基因/位點的發(fā)掘具有重要意義。目前,由于混合群體分離法的新一代測序技術(shù)（BSA-seq）可以顯著加速豆類作物的抗病基因的鑒定和發(fā)掘效率,因此采用該技術(shù)來快速鑒定大豆胞囊線蟲病的抗性基因/位點是可行的。本研究主要針對大豆胞囊線蟲病4號小種（SCN4）采用BSA-seq方法發(fā)現(xiàn)了一個新的抗性基因位點和相應(yīng)候選基因,初步驗證了基因功能,并開發(fā)出了診斷分子標(biāo)記,可應(yīng)用于大豆胞囊線蟲病抗性育種中。本研究首先利用晉豆23（敏感品種）×灰皮支黑豆（抗性品種）衍生的重組自交系群體145個家系為材料,采用胞囊指數(shù)的方法鑒定該群體對SCN4號小種的抗病指數(shù),然后依據(jù)鑒定結(jié)果構(gòu)建了各包含15個家系的抗病和感病基因池,基于BSA-seq的方法分別對抗病與感基因池以及雙親進行重測序,依據(jù)歐式距離（ED）和SNP指數(shù)（SNP-index）的方法分析比較抗感池和雙親之間的... 

【文章來源】：中國農(nóng)業(yè)科學(xué)院北京市

【文章頁數(shù)】：109 頁

【學(xué)位級別】：博士

【文章目錄】：
附件
摘要
Abstract
List of abbreviations
Chapter1 Background Information and Literature Review
    1.1 Introduction
    1.2 The life cycle of soybean cyst nematode
    1.3 SCN Population and race
    1.4 Distribution of SCN
    1.5 Management of SCN in Soybean
    1.6 Cytological and histological process of syncytium
    1.7 Germplasm screening and sources of SCN resistance
    1.8 Identification of SCN resistant QTL and genes
    1.9 Functional gene analysis studies for SCN resistance
    1.10 Molecular mechanisms of SCN resistance
    1.11 Soybean Breeding Strategies for Resistance to SCN
    1.12 Summary
    1.13 Objectives
    1.14 Technical route map
Chapter2 Candidate Genes Mining for Resistance to Soybean Cyst Nematode Race4 Based on Bulk Segregant Analysis in Glycine max
    2.1 Introduction
    2.2 Materials and methods
        2.2.1 Plant materials and phenotypic analysis
        2.2.2 Construction of Sequencing Libraries and Sequencing
        2.2.3 Identification of genomic regions and putative candidate genes for SCN
    2.3 Results
        2.3.1 Phenotypic variations and construction of extreme bulks for SCN4 resistance
        2.3.2 Whole-genome resequencing,mapping of reads and identification of SNPs
        2.3.3 Identification of candidate genomic regions for SCN4 resistance
        2.3.4 Putative candidate genes for SCN4 resistance
        2.3.5 No copy number variation was observed in rhg1-paralog
    2.4 Discussion
    2.5 Summary
Chapter3 Functional Analysis of Gm SNAP11 for Resistance to Soybean Cyst Nematode Race4 in Glycine max
    3.1 Introduction
    3.2 Materials and methods
        3.2.1 Plant materials and phenotypic analysis
        3.2.2 Sequencing and identification of genetic variation
        3.2.3 Functional analysis of Gm SNAP11 and Gm PLAC8
        3.2.4 Hairy transformation system
        3.2.5 Nematode Infection of Transgenic Hairy Roots
        3.2.6 RNA Isolation and q PCR
    3.3 Results
        3.3.1 Sequencing and identification of genetic variation of Gm SNAP11
        3.3.2 Gm SNAP11 contributes to SCN4 resistance
    3.4 Discussion
    3.5 Summary
Chapter4 Development and Utilization of KASP Markers for the Soybean Cyst Nematode Resistance loci,rhg1,Rhg4 and rhg1-paralog
    4.1 Introduction
    4.2 Materials and methods
        4.2.1 Plant materials and phenotyping
        4.2.2 SNP identification and development of Kompetitive allele-specific PCR(KASP)genotyping assays
        4.2.3 SNP marker development and validation of candidate genomic regions identified
        4.2.4 Statistical analysis
    4.3 Results
        4.3.1 Application of KASP assays for selection of resistance to SCN
        4.3.2 The presence of three resistant loci exhibit a higher effect for SCN4 resistance
    4.4 Discussion
    4.5 Summary
Chapter5 Conclusions
References
Appendix
Acknowledgements
Resume


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期刊論文
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