土壤鎘污染嚴(yán)重影響作物的產(chǎn)量和品質(zhì),并通過食物鏈威脅人類健康。對于受輕度或中度鎘污染的土壤,培育和種植耐鎘且食用器官低積累作物基因型/品種,是有效利用自然資源和保證農(nóng)產(chǎn)品安全生產(chǎn)的重要途徑之一。玉米（Zea mays L.）是目前世界上產(chǎn)量最高的糧食作物。本研究在分析比較95個玉米基因型（自交系）籽粒鎘含量篩選獲得籽粒鎘積累不同玉米基因型的基礎(chǔ)上,分析了鎘脅迫對不同籽粒鎘積累基因型玉米生長、植物激素等的影響及基因型差異,并通過比較轉(zhuǎn)錄組分析,探討了鎘耐性與低積累的相關(guān)基因。主要研究結(jié)果如下:1.玉米籽粒鎘低積累基因型篩選分析評估95份玉米基因型（自交系）籽粒鎘含量,篩選籽粒鎘低積累基因型。連續(xù)兩年的田間試驗在浙江大學(xué)長興農(nóng)業(yè)試驗站進行,分析測定籽粒鎘含量,結(jié)果表明,相同栽培條件下,95個玉米基因型籽粒鎘含量平均為0.013 mg kg^-1 DW,變化范圍為0.001-0.06 mg Cd kg^-1DW,基因型間差異顯著。連續(xù)兩年的試驗結(jié)果顯示,籽粒鎘含量基因型差異的變化趨勢完全一致,其中籽粒鎘含量最低和最高的分別為:基因型L42、L15和L5... 

【文章來源】：浙江大學(xué)浙江省 211工程院校 985工程院校 教育部直屬院校

【文章頁數(shù)】：78 頁

【學(xué)位級別】：碩士

【文章目錄】：
Acknowledgements
List of Abbreviations
ABSTRACT
摘要
CHAPTER1:Introduction
CHAPTER2:Literature review
    2.1 Cd toxicity
    2.2 Approaches for decreasing grain Cd accumulation
    2.3 Cadmium uptake,translocation and accumulation in grains
    2.4 Advances in molecular mechanisms of Cd transport and tolerance
    2.5 Research Objectives
CHAPTER3 Identification of maize genotypes with low grain Cd accumulation
    3.1 Material and Methods
        3.1.1 Field experiments design
        3.1.2 Analysis of heavy metal concentration in grains
        3.1.3 Statistical analysis
    3.2 Results
        3.2.1 Heavy metal concentrations in maize grains
        3.2.2 Correlation analysis among grain heavy metal concentrations
    3.3 Discussion
CHAPTER4 Differences in physiological features in response to Cd stress in the two maize genotypes with contrasting grain Cd accumulation
    4.1 Materials and Methods
        4.1.1 Plant materials and growth conditions
        4.1.2 Determination of heavy metal contents
        4.1.3 Measurement of plant growth parameters
        4.1.4 TEM(transmission electron microscope)ultrastructure
        4.1.5 Phytohormones determination
        4.1.6 Statistical analysis
    4.2 Results
        4.2.1 Plant growth parameters
        4.2.2 Cd content
        4.2.3 Element Analysis
        4.2.4 Phytohormone contents
        4.2.5 Examination of root ultrastructure
        4.2.6 Examination of leaf ultrastructure
    4.3 Discussion
CHAPTER5:Comparative transcription study in response to Cd stress between the two maize genotypes with contrasting grain Cd accumulation
    5.1 Materials and Methods
        5.1.1 Plant materials and growth conditions
        5.1.2 RNA isolation
        5.1.3 Library preparation and sequencing of RNAs
        5.1.4 Genome mapping
        5.1.5 qRT-PCR validation
    5.2 Results
        5.2.1 Functional categorization
        5.2.2 qRT-PCR confirms expression pattern of the transcriptome data
    5.3 Discussion
CHAPTER6 Major findings and future prospects
    6.1 Major Findings
    6.2 Future Prospects
References
Supplementary



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