目的:對卡波西肉瘤病毒（Kaposi’s sarcoma-associated herpesvirus,KSHV）編碼的小分子RNA kshv-miR-K12-1-5p進(jìn)行靶基因預(yù)測和靶基因生物信息學(xué)分析,為未來對kshv-miR-K12-1-5p靶基因的研究提供可行的方向。方法:利用數(shù)據(jù)庫PubMed、miRTarBase、TargetScanHuman Custom、miRDB等對kshv-miR-K12-1-5p靶基因進(jìn)行查詢和預(yù)測,之后對這些靶基因進(jìn)行GO和KEGG的富集分析以及蛋白互作網(wǎng)絡(luò)的分析,并對互作網(wǎng)絡(luò)的關(guān)鍵模塊和關(guān)鍵基因進(jìn)行提取和分析。結(jié)果:通過數(shù)據(jù)庫miRTarBase查詢到經(jīng)過驗證的靶基因有NF-Kappa-B抑制因子α和細(xì)胞轉(zhuǎn)錄因子MAF;通過PubMed查詢到經(jīng)過驗證的靶基因有抑制細(xì)胞因子信號轉(zhuǎn)導(dǎo)6。通過生物信息學(xué)分析預(yù)測得到8個靶基因,分別是PPIH、POLR2B、HNRNPU、MAGOHB、DNAJC8、HNRNPC、RBM22、HNRNPA3。結(jié)論:本次研究通過利用生物信息學(xué)的方法,對KSHV編碼的kshv-miR-K12-1-5p靶基因進(jìn)行預(yù)測,得到一... 

【文章來源】：腫瘤預(yù)防與治療. 2020,33(12)

【文章頁數(shù)】：7 頁

【部分圖文】：

TargetScan Human Custom和miRDB預(yù)測得到的kshv-miR-K12-1-5p靶基因

表2 關(guān)鍵基因的簡要介紹Table 2. Brief Introduction of Hubgenes No. Name Full name Brief introduction 1 PPIH Peptidylprolyl isomerase H Diseases associated with PPIH include tropical sprue and urofacial syndrome 1 2 POLR2B RNA polymerase II subunit B Diseases associated with POLR2B include Fusariosis and eumycotic mycetoma 3 HNRNPU Heterogeneous nuclear ribonucleoprotein U Diseases associated with HNRNPU include epileptic encephalopathy, early infantile, 54 and visual epilepsy 4 MAGOHB Mago homolog B, exon junction complex subunit Among its related pathways are cleavage of growing transcript in the termination region and transport of mature transcript to cytoplasm 5 DNAJC8 DnaJ heat shock protein family (Hsp40) member C8 A protein coding gene; among its related pathways are gene expression and mRNA splicing-major pathway 6 HNRNPC Heterogeneous nuclear ribonucleoprotein C Diseases associated with HNRNPC include mixed connective tissue disease and oculopharyngeal muscular dystrophy 7 RBM22 RNA binding motif protein 22 Required for pre-mRNA splicing as component of the activated spliceosome, involved in the first step of pre-mRNA splicing 8 HNRNPA3 Heterogeneous nuclear ribonucleoprotein A3 Diseases associated with HNRNPA3 include dermatopathia pigmentosa reticularis and multisystem proteinopathy圖3 使用Cytoscape 軟件MCODE插件獲取的靶基因網(wǎng)絡(luò)中最關(guān)鍵模塊

圖2 STRING數(shù)據(jù)庫獲取的kshv-miR-K12-1-5p靶基因蛋白互作網(wǎng)絡(luò)圖4 使用Cytoscape 軟件 BiNGO 插件獲得的最關(guān)鍵基因的富集結(jié)果


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