作為重要的糧食作物,水稻從傳統(tǒng)的經(jīng)驗育種向現(xiàn)代精準育種轉(zhuǎn)變的過程中,株型是影響水稻產(chǎn)量最為重要因素之一,它取決于植株高度、分蘗數(shù)目、分蘗角度、穗形態(tài)等因素。對于水稻株型的機制研究,是短期內(nèi)難以突破的科學問題和技術難題,其中矮化突變體是非常理想的研究對象,但是調(diào)控機制知之甚少。本文在前期研究的工作基礎上,對水稻矮化、少分蘗D26突變體進行系統(tǒng)研究,揭示它參與水稻油菜素內(nèi)酯激素調(diào)控的信號通路機制。本文從水稻突變體庫中組織培養(yǎng)變異獲得了2個農(nóng)藝性狀表現(xiàn)矮化、少分蘗的突變材料D26-1和D26-2,通過圖位克隆實現(xiàn)精細定位和進一步的功能分析,揭示了D26突變體矮化表型的遺傳機理,并從互作蛋白篩選和轉(zhuǎn)錄組測序分析角度,進一步挖掘水稻矮化機制。主要結果如下:D26突變體一個隱性單基因控制表型。利用D26突變體做母本和93-11親本做父本,構建雜交F₂群體,通過425個群體和531個群體將其分別定位在3號染色體短臂上63 kb區(qū)間,該區(qū)間內(nèi)包含12個候選基因;分析預測和克隆、測序表明D26基因是一個編碼GRAS家族蛋白的轉(zhuǎn)錄因子,兩個突變體在D26基因540 bp和560 bp... 

【文章來源】：華僑大學福建省

【文章頁數(shù)】：143 頁

【學位級別】：博士

【部分圖文】：

水稻油菜素內(nèi)酯相關基因研究網(wǎng)絡(Tong,2018)

D26基因分別從水稻秈稻明恢86和D26突變體的c DNA中擴增獲得全長,片段回收與pMD18-T連接、測序比對分析�；パa實驗載體構建:一個含有D26基因啟動子、c DNA全長和3"UTR非編碼區(qū)3563 bp全長片段從中間載體p UPGWPR上由HindIII/Eco RV酶切,與Bam HI酶切p CAMBIA1300載體分別回收,兩片段經(jīng)平端連接,最終構建互補載體p CAMBIAGWP26(見圖1)。2.2.8 植物葉片總RNA提取-TRIzol法

轉(zhuǎn)錄組測序流程

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