發(fā)布時(shí)間：2020-12-29 20:28

　　小麥紋枯病主要由土傳真菌Rhizoctonia cerealis侵染小麥莖基部引起。它已經(jīng)成為世界范圍內(nèi)小麥生產(chǎn)的限制性因素,在中國(guó)更為嚴(yán)重。小麥紋枯病的防治已經(jīng)成為小麥生產(chǎn)可持續(xù)發(fā)展的主要問(wèn)題。培育和種植抗性小麥品種是防治小麥紋枯病最環(huán)保、高效的方法。小麥紋枯病抗性遺傳機(jī)制研究有助于解析小麥抗性相關(guān)基因的功能和作用。本研究中,我們從抗紋枯病的小麥材料CI12633中鑒定了一個(gè)L型凝集素受體激酶基因,命名為T(mén)aLecRK-Ⅳ.1。與感紋枯病小麥溫麥6號(hào)相比,在CI12633中該基因的表達(dá)受紋枯病侵染誘導(dǎo)。序列分析表明,該基因編碼含有676個(gè)氨基酸殘基的L型凝集素受體激酶。利用病毒誘導(dǎo)基因沉默技術(shù)（virus-induced gene silencing,VIGS）技術(shù)沉默CI12633的TaLecRK-Ⅳ.1后,小麥植株表現(xiàn)矮化的表型,表明該基因可能是小麥矮化的負(fù)調(diào)控因子。進(jìn)一步在含有矮化基因Rht-D1b（formerly Rht2）的小麥中分析了該基因的轉(zhuǎn)錄,結(jié)果表面TaLecRK-Ⅳ.1在矮化小麥中的表達(dá)量很低。另外,參與植物生長(zhǎng)和發(fā)育的激素---赤霉素和生長(zhǎng)素誘導(dǎo)TaLecRK... 

【文章來(lái)源】：中國(guó)農(nóng)業(yè)科學(xué)院北京市

【文章頁(yè)數(shù)】：118 頁(yè)

【學(xué)位級(jí)別】：博士

【文章目錄】：
摘要
Abstract
Chapter 1 Introduction and literature review
    1.1 Introduction
        1.1.1 History and origin of wheat
        1.1.2 Social and economic importance
        1.1.3 Wheat cultivation around the world
        1.1.4 Influence of plant height on wheat production
        1.1.5 Role of phytohormones on plant growth
        1.1.6 Factor that favor wheat adaptability
        1.1.7 Constraints of wheat production
        1.1.8 Wheat sharp eyespot
        1.1.9 Employment of RNA-seq to identify putative sharp eyespot resistant genes
        1.1.10 Gene introgression from wheat wild relatives
        1.1.11 Importance of molecular markers in crop breeding
        1.1.12 Molecular marker development
        1.1.13 Recombinant inbred line （RIL） populations in breeding research
    1.2 Literature review
        1.2.1 Sharp eyespot
        1.2.2 Plants and disease
        1.2.3 Plant defense mechanisms
        1.2.4 Signal perception
        1.2.5 Genes mediated resistances
        1.2.6 Hypersensitive response （HR）
        1.2.7 Biochemical defenses
        1.2.8 Pathogenesis-related proteins
    1.3 Purpose of the study
Chapter 2 Silencing of L-type lectin-like receptor kinase gene Ta Lec RK-Ⅳ.1 leads to dwarf phenotype in wheat line CI12633
    Introduction
    2.1 Materials and Methods
        2.1.1 Plant and fungal materials, growth conditions and treatments
        2.1.2 RNA Sequencing-based transcriptome analysis
        2.1.3 Methodology
        2.1.4 Virus-Induced Gene Silencing
        2.1.5 BSMV inoculation
        2.1.6 Test for susceptibility to sharp eyespot
        2.1.7 Phytohormones treatment （GA, Auxin, ABA and SA）
    2.2 Results
        2.2.1 Identification of Ta Lc RK-Ⅳ.1 by RNA-Seq and cloning of the gene sequence
        2.2.2 Ta Lc RK encodes an L-type lectin domain containing receptor kinase Ⅳ.1
        2.2.3 Chromosomal location of Ta Lec RK -Ⅳ.1
        2.2.4 Phenotype resulting from the gene silencing experiment
        2.2.5 Sharp eyespot responses of the plants subjected to the VIGS experiment
        2.2.6 Relative expression of Ta Lec RK-Ⅳ.1 and its homologue gene in wheat line with Rht-D1b
        2.2.7 Analysis of the expression of GA biosynthetic enzymes Ta GA20ox and Ta GA3ox2 in Rht2 wheat line and CI12633 plants inoculated with BSMV:Ta Lec RK-Ⅳ.1
        2.2.8 Expression patterns of Ta Lec RK-Ⅳ.1 in response to exogenous hormone treatments
    Discussion
Chapter 3 Analysis for quantitative loci using molecular markers derived from wheat genes induced by sharp eyespot
    Introduction
    3.1 Materials and methods
        3.1.1 Plant materials
        3.1.2 Pathogen material
        3.1.3 Pathogen tests
        3.1.4 Pathogen assessment
        3.1.5 DNA isolation and PCR amplification
        3.1.6 Linkage map construction and QTL analysis
    3.2 Results
        3.2.1 Phenotypic assessments of sharp eyespot infection
        3.2.2 Marker development, genotyping and linkage map construction
        3.2.3 Analysis of the phenotypic and genotypic data for QTL associated with the resistance trait
    Discussion
Chapter 4 General discussion
Summary
References
Acknowledgements
Funding
Manuscript in preparation


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期刊論文
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