適時開花和成熟對于大豆來說是重要的生態(tài)和農(nóng)藝性狀,這決定了在不同環(huán)境下大豆的適應(yīng)性、生長發(fā)育、品質(zhì)及產(chǎn)量等。自然變異是進(jìn)化的主要來源,它決定了一個物種的適應(yīng)潛力。多數(shù)自然變異呈現(xiàn)數(shù)量性狀,由多個位點(diǎn)和基因的分子遺傳多樣性決定。在光周期調(diào)控的開花和發(fā)育過程中,BBX基因家族成員CONSTANS（CO）是中心調(diào)控基因,引發(fā)可移動成花素FT基因的表達(dá),進(jìn)而使得作物進(jìn)入開花階段。在大豆中,CONSTANS-LIKE基因不僅在開花階段起作用,在其他重要生理過程中也發(fā)揮了重要作用。然而,COL家族基因的自然變異與大豆開花和成熟的關(guān)系尚不明確。本研究中,選取來自14個生育期組（MG0000-MGX）的128個大豆品種作為試驗(yàn)材料。材料種植于中國北京的自然光照條件下,并對各生育時期-出苗期（VE）、初花期（R1）和完熟期（R8）進(jìn)行調(diào)查。供試大豆品種的開花期和成熟期具有顯著差異。根據(jù)基因型和生長發(fā)育結(jié)構(gòu),供試品種分為3組MG0000-MG0,MGI-MGV和MGVI-MGX。在開花期（VE-R1）方面,生育期組MG0000,MGIX+X和MGVII的大豆品種開花期差異十分顯著;成熟期（VE-R7）方面... 

【文章來源】：中國農(nóng)業(yè)科學(xué)院北京市

【文章頁數(shù)】：175 頁

【學(xué)位級別】：博士

【文章目錄】：
附件
摘要
Abstract
LIST OF ABBREVIATIONS
1 Chapter 1 Introduction
    1.1 Preamble
    1.2 Natural variation, flowering time, and maturity trait in soybean
    1.3 Photoperiodism phenomenon- remarkable findings in plant science
    1.4 Regulatory networks and genes influencing flowering time in plants
    1.5 Establishment of soybean growth season
    1.6 The purpose and significance of this study
2 Chapter 2 GmCOL Genes in Maturity and Flowering Time
    2.1 Photoperiod and relevant genes that influencing maturity and flowering
    2.2 CONSTANS and CONSTANS-Like genes
    2.3 Functional and evolutionary role of COL family gene in soybean
    2.4 Flowering time regulated by day length
    2.5 Co-relation between maturity, flowering and CONSTANS genes
    2.6 Regulatory role of COL family genes in photoperiod responsive in flowering in different species
    2.7 Identification and selection of determinate growth habit MG cultivars
3 Chapter 3 Materials and Methods
    3.1 Phenotype investigation of soybean in different maturity groups
        3.1.1 Experimental materials
        3.1.2 Methods
        3.1.3 Phenotypic evaluation
        3.1.4 Growth traits
        3.1.5 Phenotypic evaluation of stem growth habits
    3.2 Genetic Study
        3.2.1 Plant materials
        3.2.2 Genetic materials
        3.2.3 Gene identity of 21 GmCOL family genes
        3.2.4 Genomic DNA extraction
        3.2.5 Data mining and primer design
        3.2.6 PCR amplification and purification
        3.2.7 Sequencing and sequence data analysis
4 Chapter 4 Results and Analysis
    4.1 Analysis of soybean varieties in different growth stages
        4.1.1 Investigation results of growth period traits
        4.1.2 Association of flowering and maturity dates of soybean shown divergence
        4.1.3 Clustering analysis
        4.1.4 Analysis of soybean maturity group varieties growth period
        4.1.5 Soybean varieties matured differently with different beginning bloom days
    4.2 Bioinformatics analysis
        4.2.1 Construction and analysis of phylogram of COL genes in soybean
        4.2.2 Analysis and association of soybean determinacy
    4.3 Sequencing results: Analysis of COL genes in soybean varieties with different growth stages
        4.3.1 Gene sequencing results and genotype analysis
        4.3.2 Genotype distribution of GmCOL family genes in soybean cultivars
        4.3.3 Genotype number and genotype frequency of GmCOL genes
        4.3.4 GmCOL genes maturity groups and genotype distribution
        4.3.5 Statistical analysis of genotypes and phenotypes
        4.3.6 Association analysis GmCOL genotypes and pairwise comparison effect
        4.3.7 Soybean COL gene family exhibited different linkage disequilibrium
        4.3.8 Haplotype analysis of 21 soybean GmCOL family genes
        4.3.9 Haplotypes and natural variation of flowering time of GmCOL family genes…
5 Chapter 5 Discussion
    5.1 Selected soybean cultivars from different maturity groups show diversity in flowering time and maturity
    5.2 Natural variation, growth period group system and genotypes at the GmCOL loci of accessions
    5.3 Conservation and divergence of TFL1 homologs in soybean and other species
    5.4 Polymorphism of GmCOL family genes – association to flowering time and maturity…
6 Chapter 6 Summery and Conclusion
7 Chapter 7 References
Appendixes
Resume
Acknowledgements



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