【摘要】：第一代抗草甘膦轉(zhuǎn)CP4-EPSPs基因大豆GTS40-3-2是國際上應(yīng)用時(shí)間最長(zhǎng)、種植面積最大的轉(zhuǎn)基因作物。本文以6份GTS40-3-2衍生的抗草甘膦轉(zhuǎn)基因大豆新品系為親本,配制4個(gè)雜交組合,利用抗性分級(jí)法和相對(duì)株高法鑒定雜交親本及其F_(2:3)子代對(duì)草甘膦的耐受性差異,分析其抗性水平與遺傳背景的相關(guān)性。結(jié)果表明,以1230 g a.i.hm~( 2)草甘膦噴施處理時(shí),轉(zhuǎn)基因親本及其F_(2:3)子代的苗期生長(zhǎng)受草甘膦抑制不顯著,而當(dāng)噴施濃度提高至3690 g a.i.hm~( 2)和4920 g a.i.hm~( 2)時(shí)則抑制作用顯著。供試的6個(gè)雜交親本中以ZLHJ06-1568、ZLHJ10-713和ZLHJ06-698對(duì)草甘膦的耐受性相對(duì)較強(qiáng),而4個(gè)F_(2:3)組合中以ZLHJ10-713×ZLHJ06-698后代在草甘膦噴施后株高受抑制最小,對(duì)草甘膦耐受性最強(qiáng)。不同組合后代對(duì)草甘膦的耐受性普遍優(yōu)于其雙親,呈現(xiàn)出雜種優(yōu)勢(shì)。各組合后代與親本之間對(duì)草甘膦的耐受性均呈正相關(guān),但由于親本間互作效應(yīng)的不同,導(dǎo)致后代抗性水平產(chǎn)生差異。本研究表明草甘膦抗性基因CP4-EPSPs在大豆中的表達(dá)水平與其遺傳背景相關(guān)聯(lián),為利用轉(zhuǎn)基因大豆新種質(zhì)培育轉(zhuǎn)基因大豆新品種過程中目標(biāo)基因的定向選擇提供了參考依據(jù)。
[Abstract]:The first generation of glyphosate resistant CP4-EPSPs transgenic soybean GTS40-3-2 is the longest and largest transgenic crop in the world. In this paper, six new glyphosate resistant soybean lines derived from GTS40-3-2 were used as parents to prepare four hybrid combinations. The tolerance of hybrid parents and their F2:3 progenies to glyphosate were identified by resistance classification method and relative plant height method, and the correlation between resistance level and genetic background was analyzed. The results showed that glyphosate did not inhibit the seedling growth of transgenic parents and their F2:3 progenies when sprayed with 1230 g a.i.hm2 glyphosate, but the inhibitory effect was significant when the spraying concentration increased to 3690 g a.i.hm2 and 4920 g a.i.hm2.The effect of glyphosate on the seedling growth of transgenic parents and their F2:3 progenies was not significant when the spraying concentration was increased to 3690 g a.i.hm2 and 4920 g a.i.hm2.The effect of glyphosate on the seedling growth of transgenic parents and their F2:3 progenies was not significant. Among the six hybrid parents tested, ZLHJ06-1568,ZLHJ10-713 and ZLHJ06-698 had relatively strong tolerance to glyphosate, while among the four F2:3 combinations, ZLHJ10-713 脳 ZLHJ06-698 progenies had the least inhibition on plant height and the strongest tolerance to glyphosate after glyphosate spraying. The tolerance of different combinations to glyphosate was generally better than that of their parents, showing heterosis. There was a positive correlation between the tolerance of progenies and parents to glyphosate, but the resistance level of progenies was different due to the different interaction effect between parents. The results showed that the expression level of glyphosate resistance gene CP4-EPSPs in soybean was related to its genetic background, which provided a reference for the targeted selection of target genes in the process of breeding new transgenic soybean varieties.
【作者單位】： 呼倫貝爾市農(nóng)業(yè)科學(xué)研究所/國家大豆改良中心呼倫貝爾分中心;中國農(nóng)業(yè)科學(xué)院作物科學(xué)研究所/國家農(nóng)作物基因資源與遺傳改良重大科學(xué)工程/農(nóng)業(yè)部北京大豆生物學(xué)重點(diǎn)實(shí)驗(yàn)室;東北農(nóng)業(yè)大學(xué);黑龍江省農(nóng)業(yè)科學(xué)院大豆研究所;
【基金】：國家轉(zhuǎn)基因生物新品種培育重大專項(xiàng)(ZX2008004-001,ZX2011004-001,ZX2013004-001,ZX2014004-001)資助 supported by the Major Project of China on New Varieties of GMO Cultivation(ZX2008004-001,ZX2011004-001,ZX2013004-001,ZX2014004-001)
【分類號(hào)】：S565.1

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1 劉吉燾;馬曉杰;狄佳春;陳旭升;;棉花草甘膦抗性基因CP4-EPSPS的初步定位[J];江蘇農(nóng)業(yè)學(xué)報(bào);2013年03期

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本文編號(hào)：2514294