【摘要】：目的探討中藥復(fù)方固本防哮飲對哮喘緩解期小鼠氣道杯狀細胞增生與黏液分泌相關(guān)基因IRE1β、SPDEF、AGR2和mCLCA3的影響。方法結(jié)合前期研究采用卵蛋白(OVA)致敏和OVA-呼吸道合胞病毒(RSV)聯(lián)合誘導(dǎo)激發(fā)BALB/c雌性小鼠,建立哮喘緩解期動物模型,隨機分為正常組,模型組,固本防哮飲低、中、高劑量組,孟魯司特鈉組及地塞米松組。qPCR法檢測肺組織中IRE1β、SPDEF、AGR2和mCLCA3 mRNA表達水平。結(jié)果模型組小鼠肺組織中IRE1β、SPDEF、AGR2mRNA表達顯著高于正常組(P0.05),而mCLCA3mRNA表達顯著低于正常組(P0.05);固本防哮飲高、中、低劑量組,孟魯司特鈉組及地塞米松組小鼠肺組織中IRE1β、SPDEF、AGR2 mRNA水平較模型組存在不同程度的下降,而固本防哮飲低劑量組和孟魯司特鈉組小鼠肺組織中mCLCA3mRNA表達顯著高于正常組(P0.05)。結(jié)論固本防哮飲防治哮喘抑制氣道杯狀細胞增生和減少黏液分泌的作用機制可能是通過降低IRE1β、SPDEF、AGR2mRNA表達所致。
[Abstract]:Objective to investigate the effect of Guben Fangxiao decoction on the genes IRE1 尾, SPDEF,AGR2 and mCLCA3 related to airway goblet cell proliferation and mucus secretion in mice with asthma remission. Methods BALB/c female mice were induced by ovalbumin (OVA) and OVA- respiratory syncytial virus (RSV). The animal model in remission stage of asthma was established and randomly divided into normal group, model group, Gubenfangxiao Yin low, middle and high dose groups, montelukast sodium group and dexamethasone group. The expression of IRE1 尾, SPDEF,AGR2 and mCLCA3 mRNA in lung tissue was detected by qPCR. Results the expression of IRE1 尾 and SPDEF,AGR2mRNA in lung tissue of model group was significantly higher than that of normal group (P 0.05), while the expression of mCLCA3mRNA was significantly lower than that of normal group (P 0.05). The levels of IRE1 尾 and SPDEF,AGR2 mRNA in lung tissue of mice in high, middle and low dose groups, montelukast sodium group and dexamethasone group were lower than those in model group, while the expression of mCLCA3mRNA in lung tissue of mice in Gubenfangxiao decoction low dose group and montelukast sodium group was significantly higher than that in normal group (P 0.05). Conclusion the mechanism of Guben Fangxiao decoction in preventing asthma from inhibiting goblet cell proliferation and reducing mucus secretion may be caused by decreasing the expression of IRE1 尾 and SPDEF,AGR2mRNA.
【作者單位】： 江蘇省兒童呼吸疾病(中醫(yī)藥)重點實驗室南京中醫(yī)藥大學(xué)中醫(yī)兒科學(xué)研究所;
【基金】：江蘇省自然科學(xué)研究重大項目(15KJA360003) 國家自然科學(xué)基金(81473723) 江蘇省研究生創(chuàng)新課題(KYZZ15_0269)
【分類號】：R285.5

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