【摘要】：采自湖南地區(qū)的辣椒輕斑駁病毒Pepper mild mottle virus(PMMoV)樣品經(jīng)單斑分離后,根據(jù)已經(jīng)報(bào)道的PMMoV序列基因保守區(qū)設(shè)計(jì)6對(duì)簡(jiǎn)并引物,采用片段重疊法和RACE方法擴(kuò)增、克隆獲得一個(gè)全長(zhǎng)為6 356bp的湖南分離物(PMMoV-HN1,登錄號(hào):KP345899)全基因組序列,編碼4個(gè)蛋白,分別為126kD蛋白(70~3 423nt)、183kD蛋白(70~4 908nt)、28kD蛋白(4 909~5 682nt)和17.5kD蛋白(5 685~6 158nt),5′-非編碼區(qū)(5′-UTR)和3′-非編碼區(qū)(3′-UTR)分別含有69和198個(gè)堿基,其中5′-UTR存在一個(gè)序列為m7G5′pppG的甲基化核苷酸帽子結(jié)構(gòu)。一致性分析發(fā)現(xiàn)PMMoV-HN1與PMMoV其他分離物的核酸一致性為94%~99%,編碼的氨基酸一致性為94%~99%。全基因組序列系統(tǒng)進(jìn)化分析表明PMMoV-HN1分離物與中國首次報(bào)道的PMMoV-CN分離物親緣關(guān)系最近。本研究是國內(nèi)報(bào)道的第二例PMMoV全基因組序列。
[Abstract]:After single spot isolation of pepper light mottle virus Pepper mild mottle virus (PMMoV) samples collected from Hunan Province, six pairs of degenerate primers were designed according to the conserved region of PMMoV sequence gene. The fragment overlap method and RACE method were used to amplify the whole genome sequence of 6 356bp Hunan isolate (PMMoV-HN1, accession number: KP345899), encoding four proteins, 126kD protein (70 鈮，

本文編號(hào)：2506421