發(fā)布時(shí)間：2019-06-15 20:45

【摘要】：以27個(gè)雞品種為試驗(yàn)材料,通過目標(biāo)捕獲測(cè)序技術(shù)檢測(cè)NFKB1基因SNPs位點(diǎn)多態(tài)性,分析雞NFKB1基因的遺傳效應(yīng);通過最大簡(jiǎn)約法進(jìn)行系統(tǒng)進(jìn)化樹構(gòu)建,探討NFKB1基因的多態(tài)性與不同雞品種的關(guān)系。結(jié)果表明:27個(gè)雞品種的NFKB1基因上共存在233個(gè)突變位點(diǎn),其中外顯子存在6個(gè)突變位點(diǎn),顯示出豐富的基因多態(tài)性;外顯子14上的SNPs位點(diǎn)特異性存在于隱性白羽雞和AA雞,并引起了相應(yīng)的氨基酸變化,該位點(diǎn)可作為這一基因的特異性候選位點(diǎn)。同時(shí),NFKB1基因在外來雞品種與地方雞品種、蛋用型與肉用型雞品種間存在差異,為進(jìn)一步研究不同雞品種間的差異提供參考資料。
[Abstract]:The SNPs polymorphism of NFKB1 gene was detected by target capture and sequencing technique in 27 chicken breeds, and the genetic effect of NFKB1 gene was analyzed, and the relationship between NFKB1 gene polymorphism and different chicken breeds was discussed by constructing phylogenetic tree by maximum reduction method. The results showed that there were 233mutation sites in NFKB1 gene of 27 chicken breeds, of which 6 mutation sites existed in exons, showing rich gene polymorphism, and the SNPs loci in exon14 existed specifically in recessed white feather chickens and AA chickens, and caused corresponding amino acid changes, which could be used as a specific candidate site for this gene. At the same time, there were differences in NFKB1 gene between foreign chicken breeds and local chicken breeds, egg type and meat type chicken breeds, which provided reference for further study of the differences among different chicken breeds.
【作者單位】： 揚(yáng)州大學(xué)動(dòng)物科學(xué)與技術(shù)學(xué)院;中國(guó)農(nóng)業(yè)科學(xué)院家禽研究所;
【基金】：“十二五”國(guó)家科技支撐計(jì)劃項(xiàng)目(2015BAD03B03) 江蘇省科技支撐計(jì)劃項(xiàng)目(BE2013392)
【分類號(hào)】：S831.2

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