基于線粒體16S rDNA基因序列沼蝦屬的系統(tǒng)發(fā)育研究
發(fā)布時間:2019-06-14 22:35
【摘要】:為了解沼蝦屬Macrobrachium各種間的系統(tǒng)發(fā)育關(guān)系,本文通過PCR方法擴(kuò)增羅氏沼蝦M.rosenbergii線粒體16SrDNA基因片段,并測序。通過比對發(fā)現(xiàn),511個位點中,可變位點178個,簡約信息位點162個。在優(yōu)化的模型中,堿基頻率為ηA=0.306 3,ηC=0.065 7,ηG=0.204 6,ηT=0.423 3;替代矩陣為[A-C]=0.042 7,[A-G]=0.248 59,[A-T]=0.028 4,[C-G]=0.032 9,[C-T]=0.629 0,[G-T]=0.018 4;不變位點的比例為0.428 7,變異位點的Gamma參數(shù)為0.429 8。在所有物種中,發(fā)現(xiàn)轉(zhuǎn)換值高于顛換值,16S基因的轉(zhuǎn)換和顛換未達(dá)到飽和。利用貝葉斯法構(gòu)建了Bayes樹,長臂蝦屬Palaemon與其他沼蝦屬構(gòu)成了顯著的并系類群。所有的物種聚成3個大的分支,其中馬來亞沼蝦M.malayanum構(gòu)成一個大分支,真沼蝦M.carcinus、美洲沼蝦M.americanum和非洲沼蝦M.vollenhovenii構(gòu)成另一個大的分支,其余32個沼蝦品種構(gòu)成第三大分支。而羅氏沼蝦與大和沼蝦M.japonicum、南方沼蝦M.meridionalis、細(xì)額沼蝦M.gracilirostre、嘉羅沼蝦M.jaroense、霍氏沼蝦M.horstii、熱帶沼蝦M.placidulum、擬鱗指沼蝦M.lepidactyloides、貪食沼蝦M.lar、寬掌沼蝦M.latimanus、俄亥俄沼蝦M.ohione形成一個群集。
[Abstract]:In order to understand the phylogenetic relationship between the genus Macrobrachium, the M.rosenbergii mtDNA 16SrDNA gene fragment was amplified by PCR and sequenced. Among the 511 loci, 178 were variable loci and 162 were minimized information sites. In the optimized model, the base frequency is 畏 A 鈮,
本文編號:2499751
[Abstract]:In order to understand the phylogenetic relationship between the genus Macrobrachium, the M.rosenbergii mtDNA 16SrDNA gene fragment was amplified by PCR and sequenced. Among the 511 loci, 178 were variable loci and 162 were minimized information sites. In the optimized model, the base frequency is 畏 A 鈮,
本文編號:2499751
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