【摘要】：杜仲是世界上唯一的硬橡膠樹種,是能夠替代三葉橡膠的最具開發(fā)潛力的膠源植物。本研究以杜仲膠合成下游途徑Ref基因?yàn)檠芯繉ο?運(yùn)用Codon W和CUSPS等研究Ref基因的密碼子組成特點(diǎn)及使用偏好性。研究結(jié)果表明,杜仲Ref基因的GC含量為49%,密碼子第三位堿基的GC含量為59%;杜仲Ref基因有28個(gè)偏好使用的密碼子,其中約60%(17個(gè))的密碼子以G/C結(jié)尾;與大腸桿菌、酵母、擬南芥和煙草的基因組密碼子使用偏好性比較,杜仲與擬南芥基因組密碼子的使用偏性更為接近,擬南芥更適合作為Ref轉(zhuǎn)基因的受體。本研究為預(yù)測杜仲Ref基因的異源表達(dá),適宜表達(dá)系統(tǒng)的選擇以及Ref基因密碼子的改造以提高其在宿主中的表達(dá),進(jìn)行有效的基因功能驗(yàn)證提供理論參考。
[Abstract]:Eucommia ulmoides is the only hard rubber tree in the world, and is the most developing potential gum source plant capable of replacing trifoliate rubber. In this study, the Ref gene of the downstream pathway of Eucommia ulmoides gum was used as the research object, and the Codon W and the CUSPS were used to study the codon composition and preference of the Ref gene. The results showed that the GC content of the Ref gene of the Eucommia ulmoides was 49%, the GC content of the third base of the codon was 59%, there were 28 codons in the Eucommia ulmoides's Ref gene, of which about 60% (17) of the codons were at the end of G/ C; and E. coli, the yeast, The using preference of the genomic codons of Arabidopsis and tobacco is closer to the use of the genomic codons of the eucommia and the Arabidopsis, and the arabidopsis is more suitable as a receptor for the Ref transgene. The study is to predict the heterologous expression of the Ref gene of the Eucommia ulmoides, the selection of the appropriate expression system and the transformation of the Ref gene codon to improve the expression of the Ref gene in the host, and to provide the theoretical reference for the validation of the gene function.
【作者單位】： 中國林業(yè)科學(xué)研究院經(jīng)濟(jì)林研究開發(fā)中心;國家林業(yè)局杜仲工程技術(shù)研究中心;
【基金】：國家林業(yè)公益性行業(yè)科研專項(xiàng)(201004029)
【分類號】：S567.19
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本文編號：2499345