拉布拉多犬髖關(guān)節(jié)發(fā)育不良和神經(jīng)類型相關(guān)基因SNP位點(diǎn)的檢測(cè)
發(fā)布時(shí)間：2019-06-12 08:12
【摘要】：目的:1、檢測(cè)拉布拉多犬基因組DNA中與髖關(guān)節(jié)發(fā)育不良密切相關(guān)的5個(gè)基因中的6個(gè)SNP位點(diǎn),以期為拉布拉多犬的選育提供有效的遺傳學(xué)診斷方法。2、檢測(cè)拉布拉多犬基因組DNA中與神經(jīng)類型密切相關(guān)的5個(gè)基因中的7個(gè)SNP位點(diǎn),以期為拉布拉多犬神經(jīng)類型的早期鑒定提供分子遺傳學(xué)鑒定指標(biāo)。方法:本研究所選用的犬和其信息均由中國(guó)導(dǎo)盲犬大連培訓(xùn)基地(以下簡(jiǎn)稱基地)提供;1、經(jīng)影像學(xué)診斷,篩選出典型的6只髖關(guān)節(jié)發(fā)育不良和11只正常拉布拉多犬作為實(shí)驗(yàn)對(duì)象,采取靜脈血,提取血細(xì)胞的基因組DNA樣本,采用PCR/測(cè)序方法對(duì)17個(gè)樣本的5個(gè)基因中的6個(gè)SNP位點(diǎn):LAMA2(CFA1:70997779),LRR1(CFA8:29247021),SCR(CFA24:28944481),COL6A3(CFA25:51031100,51040259)和FN1(CFA37:25095511)進(jìn)行變異型檢測(cè),并計(jì)算分型所占的百分比,分析正常犬和髖關(guān)節(jié)發(fā)育不良犬的差異趨勢(shì)。2、依據(jù)基地成熟的導(dǎo)盲犬神經(jīng)類型行為學(xué)評(píng)估標(biāo)準(zhǔn),選取典型的安靜、活潑、膽小型(弱型)三種神經(jīng)類型的犬各6只為研究對(duì)象,采取靜脈血,提取血細(xì)胞的基因組DNA樣本,采用PCR-RFLP分析法和PCR/測(cè)序方法對(duì)18個(gè)樣本5個(gè)基因的7個(gè)SNP位點(diǎn):COMT(G482A)、MAOB(T199C)、SLC1A2(T471C)、ABCB1(A985T、T3442C、377-378 ins C)、GNB1L(961-962 ins G)進(jìn)行變異型檢測(cè),并統(tǒng)計(jì)分析SNP位點(diǎn)的變異型與神經(jīng)類型的關(guān)聯(lián)度。結(jié)果:1、LAMA2(CFA1:70997779),LRR1(CFA8:29247021),SCR(CFA24:28944481),COL6A3(CFA25:51031100)和FN1(CFA37:25095511)SNP位點(diǎn)的變異型與拉布拉多犬的髖關(guān)節(jié)發(fā)育不良沒有顯著差異趨勢(shì)。COL6A3(CFA25:51040259)SNP位點(diǎn)的A/A型與拉布拉多犬的髖關(guān)節(jié)發(fā)育不良存在明顯的差異趨勢(shì)(30%)。2、COMT基因的G482A SNP位點(diǎn)G/G型在安靜型犬中所占比例顯著高于膽小型(P=0.026),但該兩種類型的犬所占比例與活潑型均不存在顯著性差異(P0.05);G/A型在膽小型犬中所占比例顯著高于活潑型和安靜型(P=0.011,P=0.011);A/A型在活潑型犬中所占比例顯著高于安靜型和膽小型(P=0.008,P=0.008)。MAOB基因的T199C SNP位點(diǎn)在安靜、活潑、膽小這三種類型中所占比例均沒有顯著差異(P0.05)。SLC1A2基因的T471C SNP位點(diǎn)T/T型在活潑型犬中所占比例顯著高于安靜型和膽小型(P=0.026,P=0.006);T/C型在活潑型犬中所占比例顯著低于安靜型和膽小型(P=0.023,P=0.011);C/C型在活潑型犬中所占比例顯著低于安靜型和膽小型(P=0.008,P=0.008)。ABCB1基因的A985T SNP位點(diǎn)T/T型在活潑型犬中所占比例顯著高于膽小型(P=0.014),但該兩種類型的犬所占比例與安靜型均不存在顯著性差異(P0.05);T/A型在活潑型犬中所占比例顯著低于安靜型和膽小型(P=0.005,P=0.002);而A/A型在安靜、活潑、膽小這三種類型中所占比例均沒有顯著差異(P0.05)。ABCB1基因的T3442C SNP位點(diǎn)T/T型在活潑型犬中所占比例顯著高于膽小型(P=0.045),但該兩種類型的犬所占比例與安靜型均不存在顯著性差異(P0.05);T/C型在膽小型、安靜型、活潑型犬中所占比例均存在顯著性差異(P0.05),且膽小型所占比例最高(50%),活潑型所占比例最低(0%);C/C型在安靜、活潑、膽小這三種類型中所占比例均沒有顯著差異(P0.05)。ABCB1基因的377-378 ins C和GNB1L基因的961-962ins G均沒有發(fā)現(xiàn)顯著性差異(P0.05)。結(jié)論:1、COL6A3(CFA25:51040259)SNP位點(diǎn)的A/A型可能成為拉布拉多犬髖關(guān)節(jié)發(fā)育不良的實(shí)用性遺傳學(xué)診斷指標(biāo),但尚需大樣本確認(rèn)。2、COMT基因的G482A SNP位點(diǎn)的G/A型和A/A型,SLC1A2基因的T471 SNP位點(diǎn),ABCB1基因的A985T SNP位點(diǎn)的T/A和ABCB1基因的T3442C SNP位點(diǎn)的T/C型有望成為拉布拉多犬神經(jīng)類型早期鑒定的分子遺傳學(xué)診斷指標(biāo),但尚需大樣本確認(rèn)。
[Abstract]:Objective:1. To detect the 6 SNP sites in 5 genes closely related to the dysplasia of the hip in the Labrador genomic DNA, with a view to providing an effective genetic diagnosis method for the breeding of Labrador. Seven SNP sites in the 5 genes closely related to the type of nerve in the Labrador genomic DNA were detected, with a view to providing molecular genetic identification for the early identification of the Labrador type of nerve type. Methods: The dog and its information selected by the Institute were provided by the Dalian Training Base (hereinafter referred to as the base) of the Chinese guide dog;1. According to the image diagnosis, the typical 6 total hip dysplasia and 11 normal Labrador were selected as experimental subjects, and venous blood was taken. The genomic DNA samples of the blood cells were extracted and six SNP sites in the five genes of the 17 samples were analyzed by a PCR/ sequencing method: LAM2 (CFA1:70997779), LRR1 (CFA8:29247021), SCR (CFA24:28944481), COL6A3 (CFA25:51031100,51040259) and FN1 (CFA37:25095511), and the percentage of the typing was calculated, The differences of dogs in normal and hip dysplasia were analyzed.2. The typical quiet, active and timid (weak) three types of dogs were selected for the purpose of study, and venous blood was taken for each of the three types of dogs of the type of quiet, active and timid (weak type). The genomic DNA samples of blood cells were extracted, and 7 SNP sites of five genes of 18 samples were detected by PCR-RFLP and PCR/ sequencing methods: COMT (G482A), MAOB (T199C), SLC1A2 (T471C), ABCB1 (A985T, T3442C,377-378 ins C), and GNB1L (961-962 ins G). And the incidence degree of the variant and the nerve type of the SNP locus is analyzed. Results:1, LAM2 (CFA1:70997779), LRR1 (CFA8:29247021), SCR (CFA24:28944481), COL6A3 (CFA25:51031100) and FN1 (CFA37:25095511) SNP site did not have a significant difference in the hip dysplasia of the Labrador. There was a significant difference (30%) between the type A/ A of COL6A3 (CFA25:51040259) and the dysplasia of the hip in the Labrador dog (30%). The proportion of the G482A SNP site G/ G of the COMT gene in the quiet dog was significantly higher than that of the cowardly dog (P = 0.026). However, there was no significant difference between the two types of dogs (P0.05); the proportion of the G/ A type in the cowardly dog was significantly higher than that of the active and the quiet type (P = 0.011, P = 0.011); the proportion of the A/ A in the active dog was significantly higher than that of the quiet and timid (P = 0.008, P = 0.008). The T41C SNP site of the MAOB gene has no significant difference in the three types of quiet, active and timid (P0.05). The proportion of the T471C SNP site T/ T of the SLC1A2 gene in the active dog is significantly higher than that of the quiet and timid (P = 0.026, P = 0.006); The proportion of T/ C in the active dog was significantly lower than that of the quiet and timid (P = 0.023, P = 0.011); the proportion of C/ C in the active dog was significantly lower than that of the quiet and timid (P = 0.008, P = 0.008). The proportion of A985T SNP site T/ T in the ABCB1 gene was significantly higher in the active dog (P = 0.014), but there was no significant difference between the two types of dogs (P = 0.014); the proportion of the T/ A type in the active dog was significantly lower than that of the quiet and timid (P = 0.005, P = 0.002); The ratio of type A/ A in the three types of quiet, active and timid (P0.05). The proportion of T/ T in the T/ T type of the ABCB1 gene in the active dog was significantly higher than that of the cowardly type (P = 0.045), but there was no significant difference between the two types of dogs (P0.05). The proportion of T/ C in the timid, quiet and active dogs was significant (P0.05), and the proportion of the timid (50%) and the active type was the lowest (0%), and the C/ C type was quiet and active. There was no significant difference in the three types of the cowardice (P0.05). No significant difference was found between the 377-378 ins C of the ABCB1 gene and 961-962 ins G of the GNB1L gene (P0.05). Conclusion:1. The A/ A type of the SNP site of COL6A3 (CFA25:51040259) may be a practical genetic diagnostic index for the development of the hip joint in the Labrador, but it still needs to be confirmed by large samples. The G/ A type of the G482A SNP site of the COMT gene and the T471 SNP site of the A/ A type and the SLC1A2 gene, The T/ C type of the T3442C SNP site of the A985T SNP site of the ABCB1 gene is expected to be a molecular genetic diagnostic index for the early identification of the Labrador nerve type, but a large sample confirmation is required.
【學(xué)位授予單位】：大連醫(yī)科大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2016
【分類號(hào)】：S858.292
[Abstract]:Objective:1. To detect the 6 SNP sites in 5 genes closely related to the dysplasia of the hip in the Labrador genomic DNA, with a view to providing an effective genetic diagnosis method for the breeding of Labrador. Seven SNP sites in the 5 genes closely related to the type of nerve in the Labrador genomic DNA were detected, with a view to providing molecular genetic identification for the early identification of the Labrador type of nerve type. Methods: The dog and its information selected by the Institute were provided by the Dalian Training Base (hereinafter referred to as the base) of the Chinese guide dog;1. According to the image diagnosis, the typical 6 total hip dysplasia and 11 normal Labrador were selected as experimental subjects, and venous blood was taken. The genomic DNA samples of the blood cells were extracted and six SNP sites in the five genes of the 17 samples were analyzed by a PCR/ sequencing method: LAM2 (CFA1:70997779), LRR1 (CFA8:29247021), SCR (CFA24:28944481), COL6A3 (CFA25:51031100,51040259) and FN1 (CFA37:25095511), and the percentage of the typing was calculated, The differences of dogs in normal and hip dysplasia were analyzed.2. The typical quiet, active and timid (weak) three types of dogs were selected for the purpose of study, and venous blood was taken for each of the three types of dogs of the type of quiet, active and timid (weak type). The genomic DNA samples of blood cells were extracted, and 7 SNP sites of five genes of 18 samples were detected by PCR-RFLP and PCR/ sequencing methods: COMT (G482A), MAOB (T199C), SLC1A2 (T471C), ABCB1 (A985T, T3442C,377-378 ins C), and GNB1L (961-962 ins G). And the incidence degree of the variant and the nerve type of the SNP locus is analyzed. Results:1, LAM2 (CFA1:70997779), LRR1 (CFA8:29247021), SCR (CFA24:28944481), COL6A3 (CFA25:51031100) and FN1 (CFA37:25095511) SNP site did not have a significant difference in the hip dysplasia of the Labrador. There was a significant difference (30%) between the type A/ A of COL6A3 (CFA25:51040259) and the dysplasia of the hip in the Labrador dog (30%). The proportion of the G482A SNP site G/ G of the COMT gene in the quiet dog was significantly higher than that of the cowardly dog (P = 0.026). However, there was no significant difference between the two types of dogs (P0.05); the proportion of the G/ A type in the cowardly dog was significantly higher than that of the active and the quiet type (P = 0.011, P = 0.011); the proportion of the A/ A in the active dog was significantly higher than that of the quiet and timid (P = 0.008, P = 0.008). The T41C SNP site of the MAOB gene has no significant difference in the three types of quiet, active and timid (P0.05). The proportion of the T471C SNP site T/ T of the SLC1A2 gene in the active dog is significantly higher than that of the quiet and timid (P = 0.026, P = 0.006); The proportion of T/ C in the active dog was significantly lower than that of the quiet and timid (P = 0.023, P = 0.011); the proportion of C/ C in the active dog was significantly lower than that of the quiet and timid (P = 0.008, P = 0.008). The proportion of A985T SNP site T/ T in the ABCB1 gene was significantly higher in the active dog (P = 0.014), but there was no significant difference between the two types of dogs (P = 0.014); the proportion of the T/ A type in the active dog was significantly lower than that of the quiet and timid (P = 0.005, P = 0.002); The ratio of type A/ A in the three types of quiet, active and timid (P0.05). The proportion of T/ T in the T/ T type of the ABCB1 gene in the active dog was significantly higher than that of the cowardly type (P = 0.045), but there was no significant difference between the two types of dogs (P0.05). The proportion of T/ C in the timid, quiet and active dogs was significant (P0.05), and the proportion of the timid (50%) and the active type was the lowest (0%), and the C/ C type was quiet and active. There was no significant difference in the three types of the cowardice (P0.05). No significant difference was found between the 377-378 ins C of the ABCB1 gene and 961-962 ins G of the GNB1L gene (P0.05). Conclusion:1. The A/ A type of the SNP site of COL6A3 (CFA25:51040259) may be a practical genetic diagnostic index for the development of the hip joint in the Labrador, but it still needs to be confirmed by large samples. The G/ A type of the G482A SNP site of the COMT gene and the T471 SNP site of the A/ A type and the SLC1A2 gene, The T/ C type of the T3442C SNP site of the A985T SNP site of the ABCB1 gene is expected to be a molecular genetic diagnostic index for the early identification of the Labrador nerve type, but a large sample confirmation is required.
【學(xué)位授予單位】：大連醫(yī)科大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2016
【分類號(hào)】：S858.292
【參考文獻(xiàn)】
相關(guān)期刊論文 前10條
1 趙明媛;韓芳;李雅嬋;俞劍熊;張雅麗;周子娟;王福金;王愛國(guó);王靖宇;;拉布拉多犬髖關(guān)節(jié)發(fā)育不良相關(guān)基因的SNP位點(diǎn)的檢測(cè)[J];實(shí)驗(yàn)動(dòng)物科學(xué);2015年04期
2 趙明媛;俞劍熊;韓芳;李雅嬋;張雅麗;周子娟;王福金;王愛國(guó);王靖宇;;拉布拉多犬神經(jīng)類型相關(guān)基因SNP的分析[J];實(shí)驗(yàn)動(dòng)物科學(xué);2015年03期
3 俞劍熊;張雅麗;周子娟;趙明媛;韓芳;王福金;王愛國(guó);王靖宇;;犬毛色、毛色基因型及性別與導(dǎo)盲犬培訓(xùn)成功率的相關(guān)性研究[J];實(shí)驗(yàn)動(dòng)物科學(xué);2014年06期
4 俞劍熊;張雅麗;周子娟;王福金;王愛國(guó);王靖宇;;拉布拉多犬與金毛獵犬毛色基因MC1R(R306ter)與TYRP1(Q331ter)SNP位點(diǎn)的檢測(cè)[J];實(shí)驗(yàn)動(dòng)物科學(xué);2014年03期
5 王福金;王愛國(guó);王亮;李慧玲;董建一;王靖宇;;偏側(cè)性測(cè)試在預(yù)測(cè)成功導(dǎo)盲犬中的應(yīng)用[J];實(shí)驗(yàn)動(dòng)物科學(xué);2012年03期
6 王愛國(guó);王福金;王亮;李慧玲;董建一;王靖宇;;氣質(zhì)測(cè)試在導(dǎo)盲犬培育中的應(yīng)用[J];畜牧與獸醫(yī);2012年02期
7 馬昆;張孌松;盧德章;范宏剛;馬海濵;王洪斌;;犬髖關(guān)節(jié)發(fā)育不良的診斷、遺傳控制和預(yù)防管理[J];黑龍江畜牧獸醫(yī);2011年03期
8 馮爍;沈向真;;犬髖關(guān)節(jié)發(fā)育異常的研究進(jìn)展[J];中國(guó)畜牧獸醫(yī);2010年08期
9 杜曉鵬;顧_g;;賞析拉布拉多犬與金毛獵犬[J];中國(guó)工作犬業(yè);2010年07期
10 翟，
本文編號(hào)：2497866
本文鏈接：http://sikaile.net/kejilunwen/jiyingongcheng/2497866.html
最近更新
教材專著
