【摘要】：目的:檢測肺癌患者血清中抑癌基因p16、FHIT、APC的甲基化,探討它們在肺癌發(fā)生和診斷中的臨床價值。方法:收集120例原發(fā)性肺癌患者(肺癌組)、46例肺良性疾病患者(對照組)的血液標(biāo)本,采用甲基化PCR方法檢測2組患者血清p16、FHIT、APC基因甲基化。采用logistic回歸分析肺癌發(fā)生的危險因素,計(jì)算上述基因甲基化診斷肺癌的準(zhǔn)確率。結(jié)果:肺癌組血清中p16、FHIT、APC的甲基化率分別為37.5%、34.2%、31.7%,對照組中分別為6.5%、13.0%、10.9%,差異有統(tǒng)計(jì)學(xué)意義(P均0.05)。Logistic回歸分析提示p16、FHIT基因甲基化均為肺癌發(fā)生的獨(dú)立危險因素,OR(95%CI)分別為7.509(2.160~26.099)、2.927(1.096~7.814)(P均0.05)。p16、FHIT、APC基因甲基化單項(xiàng)診斷肺癌的準(zhǔn)確率分別為53%、49%、47%,3種基因甲基化聯(lián)合診斷肺癌的準(zhǔn)確率為87%,優(yōu)于單項(xiàng)指標(biāo)。結(jié)論:p16、FHIT基因甲基化均為肺癌發(fā)生的獨(dú)立危險因素;聯(lián)合檢測p16、FHIT、APC甲基化可提高肺癌診斷的準(zhǔn)確性。
[Abstract]:Objective: To detect the methylation of p16, FHIT and APC in the serum of patients with lung cancer and to explore their clinical value in the occurrence and diagnosis of lung cancer. Methods: The blood samples of 120 patients with primary lung cancer (lung cancer) and 46 patients with benign lung disease (control group) were collected. The methylation of p16, FHIT and APC gene was detected by methylation PCR. The risk factors of lung cancer were analyzed by logistic regression, and the accuracy of the above-mentioned gene methylation in the diagnosis of lung cancer was calculated. Results: The methylation rates of p16, FHIT and APC in the serum of the lung cancer group were 37.5%, 34.2%, 31.7%, and 6.5%, 13.0% and 10.9% in the control group, respectively (P <0.05). Logistic regression analysis suggested that the methylation of p16 and FHIT gene was an independent risk factor for lung cancer, OR (95% CI) was 7.509 (2.160-26.099), 2.927 (1.096-7.814) (P <0.05). The accuracy of p16, FHIT and APC gene methylation in the diagnosis of lung cancer was 53%,49% and 47%, respectively. Conclusion: The methylation of p16 and FHIT is an independent risk factor for lung cancer, and the detection of p16, FHIT and APC methylation can improve the accuracy of the diagnosis of lung cancer.
【作者單位】： 鄭州大學(xué)第一附屬醫(yī)院呼吸科;鄭州大學(xué)公共衛(wèi)生學(xué)院勞動衛(wèi)生學(xué)教研室;
【基金】：河南省教育廳科技攻關(guān)項(xiàng)目12A320020 河南省科技廳創(chuàng)新人才項(xiàng)目154200510015
【分類號】：R734.2

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