【摘要】：為分析山西地區(qū)豬流行性腹瀉病毒(PEDV)的遺傳變異情況,試驗(yàn)利用RT-PCR方法對(duì)2014-2015年山西省疑似豬流行性腹瀉的陽(yáng)性病料進(jìn)行克隆和測(cè)序,獲得4個(gè)S基因片段,并對(duì)其基因序列和推導(dǎo)的氨基酸序列與國(guó)內(nèi)外毒株進(jìn)行比對(duì)分析。序列分析結(jié)果顯示,4株P(guān)EDV山西分離株的S基因與CV777vaccine相比,在170~171bp之間插入12個(gè)核苷酸,在401~402、454~455bp之間均插入3個(gè)核苷酸,在461~468bp之間缺失6個(gè)核苷酸。4株P(guān)EDV山西分離株S基因之間核苷酸和氨基酸同源性分別為99.2%~99.8%和98.6%~99.7%,與2011-2015年中國(guó)流行毒株、CV777vaccine、attenuated DR13、CV777的核苷酸同源性分別95.0%~98.5%、93.2%~93.6%、93.2%~93.7%、93.7%~94.4%,氨基酸同源性分別為96.2%~98.9%、91.9%~92.6%、92.1%~92.9%、92.9%~94.0%。遺傳進(jìn)化樹分析結(jié)果表明,PEDVS基因分為3個(gè)群,4株P(guān)EDV山西分離株屬于第一群,與2010年以后國(guó)內(nèi)流行毒株(除AH-M、SQ2014)的親緣關(guān)系較近,與2010年以前中國(guó)流行毒株、2個(gè)日本株、7個(gè)韓國(guó)株、2個(gè)疫苗株的親緣關(guān)系較遠(yuǎn)。研究結(jié)果提示山西省流行的PEDV發(fā)生較明顯的變異,需研發(fā)新的疫苗來(lái)控制PEDV的暴發(fā)。
[Abstract]:In order to analyze the genetic variation of swine epidemic diarrhea virus (PEDV) in Shanxi Province, four S gene fragments were obtained by RT-PCR cloning and sequencing of suspected swine epidemic diarrhea positive materials in Shanxi Province from 2014 to 2015. The gene sequence and deduced amino acid sequence were compared with those of domestic and foreign strains. The results of sequence analysis showed that compared with CV777vaccine, the S gene of 4 PEDV Shanxi isolates inserted 12 nucleotides between 170~171bp and 3 nucleotides between 401~402454~455bp. Six nucleotides were deleted between 461~468bp. The homology of nucleotides and amino acids between S genes of four PEDV Shanxi isolates was 99.2% 鈮，

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