【摘要】：目的:探討染料木素對UVA所致成纖維細胞凋亡過程中Sirt1基因表達的影響。方法:為了保證實驗的準確性和可重復性,本實驗將成纖維細胞分為7組,進行對照試驗,其中A組為空白對照組(不添加染料木素,不照射UVA),剩余B至G組均照射UVA,具體劃分是:B組為UVA照射對照組(不添加染料木素);實驗組包括C組~G組,其實驗藥物的濃度分別為0.01μmol/L、0.1μmol/L、1μmol/L、10μmol/L和100μmol/L。采用MTT比色法檢測成纖維細胞在UVA照射下染料木素對其增殖活性的影響,采用RT-PCR檢測染料木素對Sirt1 m RNA的表達水平的變化。結果:與B(0.401±0.011)組比較,C~F(0.672±0.054、0.863±0.021、0.887±0.052、0.583±0.023)組細胞增值活性有明顯升高,統(tǒng)計學上差異顯著(P0.05),各實驗組中D~E(0.863±0.021、0.887±0.052)組的細胞增值活性最高,統(tǒng)計學上差異顯著(P0.05);與B(0.713±0.019)組比較,C~F(0.864±0.062、1.026±0.065、1.096±0.054、0.876±0.020)組Sirt1表達顯著升高,統(tǒng)計學上有顯著差異(P0.05),其中E組的Sirt1表達量顯示最多,統(tǒng)計學上差異顯著(P0.05),而B組與G(0.704±0.029)組相對照,Sirt1 m RNA的表達量無統(tǒng)計學差異(P0.05)。結論:染料木素在一定濃度范圍內(0.01μmol/L~10μmol/L)對UVA致成纖維細胞的凋亡有防護作用,并且對成纖維細胞的Sirt1 m RNA的表達有顯著增強作用,其中在濃度為0.1μmol/L~1μmol/L內保護作用最強。
[Abstract]:Aim: to investigate the effect of genistein on Sirt1 gene expression during apoptosis of fibroblasts induced by UVA. Methods: in order to ensure the accuracy and reproducibility of the experiment, fibroblasts were divided into 7 groups and control trial was carried out. Group A was blank control group (no genistein was added, no UVA), residual B to G group was irradiated with UVA,). The specific division is as follows: group B is UVA irradiation control group (no genistein added); The experimental group included group C ~ G. The concentrations of the experimental drugs were 0.01 渭 mol / L, 0.1 渭 mol / L, 1 渭 mol / L, 10 渭 mol / L and 100 渭 mol / L, respectively. MTT colorimetric assay was used to detect the effect of genistein on the proliferation activity of fibroblasts irradiated by UVA, and RT-PCR was used to detect the expression of Sirt1 m RNA by genistein. Results: compared with group B (0.401 鹵0.011), C 鈮，

本文編號：2481990