【摘要】：為了解慈竹WRKY生物學功能以及通過基因工程手段改良竹類植物。利用已獲得慈竹轉(zhuǎn)錄組數(shù)據(jù),克隆獲得2條WRKY轉(zhuǎn)錄因子,分別命名為BeWRKY1(Gen Bank:KJ462124)和Be WRKY2(Gen Bank:KJ462125),兩者分別編碼189和295個氨基酸殘基。序列分析表明,兩者編碼的蛋白均含有一個完整的WRKY superfamily的保守結(jié)構(gòu)域。BeWRKY1屬于Ⅱc亞家族;Be WRKY2則與Ta WRKY16和AtWRKY39等聚為一枝,屬于Ⅱd亞家族。定量PCR分析表明,Be WRKY1和Be WRKY2分別在莖和完全展開葉中具有較高表達量。200mmol·L~( 1)NaCl和20%PEG 6000脅迫處理激活了Be WRKY1表達,卻減少了BeWRKY2的轉(zhuǎn)錄積累。結(jié)果表明BeWRKY1可能短時間內(nèi)迅速參與逆境響應;Be WRKY2對干旱和ABA脅迫傷害更為敏感,而可能不參與高鹽類脅迫保護反應。
[Abstract]:In order to understand the biological function of Phyllostachys pubescens WRKY and improve bamboo plants by genetic engineering. Based on the obtained data of Zizhu transcriptional group, two WRKY transcription factors, named BeWRKY1 (Gen Bank:KJ462124) and Be WRKY2 (Gen Bank:KJ462125), were cloned, which encoded 189 and 295 amino acid residues, respectively. Sequence analysis showed that the proteins encoded by the two proteins contained a complete conserved domain of WRKY superfamily. BeWRKY1 belonged to the 鈪，

本文編號：2478560