【摘要】：在進行馬尾松抗蟲材料轉(zhuǎn)錄組測序分析時發(fā)現(xiàn)一個編碼CYP77A1的cDNA,命名為PmCYP77A1。序列分析結(jié)果顯示,該基因全長1 620 bp,編碼539個氨基酸。氨基酸序列分析表明其具有CYP450家族基因的保守結(jié)構(gòu)域,但CYP77A1基因中的FXXGXXXCXG保守特征序列最后一個堿基被A堿基所代替。與其他植物核酸序列進行比對覆蓋率和同源性均較差,而氨基酸序列比對覆蓋率達到88%~96%,但同源性不高,基本都在50%~55%之間,PmCYP77A1與白云杉遺傳聚類最近,與其他被子植物遺傳聚類較遠。利用熒光定量PCR技術(shù)分析得知,PmCYP77A1在馬尾松的不同組織中均有表達,其中在嫩莖、雌雄花和嫩葉中的表達量較高,而在球果、成熟組織和根系中的表達量最低。在不同抗蟲材料日變化中PmCYP77A1表達量出現(xiàn)先升高后降低的表達趨勢,該基因在抗蟲材料中表達啟動早。研究結(jié)果將為進一步研究PmCYP77A1的生物學功能及在馬尾松抗蟲防御中的作用機理奠定基礎(chǔ)。
[Abstract]:In the sequencing analysis of Pinus massoniana insect-resistant material transcriptional group, it was found that a cDNA, encoding CYP77A1 was named PmCYP77A1.. The results of sequence analysis showed that the full length of the gene was 1620 bp, encoding 539 amino acids. Amino acid sequence analysis showed that it had the conserved domain of CYP450 family gene, but the last base of FXXGXXXCXG conserved characteristic sequence in CYP77A1 gene was replaced by A base. The coverage and homology of nucleic acid sequences were poor with other plant nucleic acid sequences, but the amino acid sequence ratio was 88% 鈮，

本文編號：2478145