【摘要】：CRISPR-Cas9系統(tǒng)自問世以來一直被廣泛地應(yīng)用于基因組編輯技術(shù)中,直到人們通過點(diǎn)突變的方式將Cas9的Ruv C和HNH剪切結(jié)構(gòu)域失活得到d Cas9后,這個(gè)系統(tǒng)又有了新的功能——調(diào)節(jié)基因的表達(dá)水平。研究者通過改變g RNA的結(jié)構(gòu),并與RNA結(jié)合蛋白配對(duì),從而招募轉(zhuǎn)錄因子以調(diào)節(jié)基因的轉(zhuǎn)錄水平,達(dá)到影響基因表達(dá)的目的。本文主要討論了CRISPR-d Cas9系統(tǒng)的結(jié)構(gòu)特點(diǎn)、作用原理和目前對(duì)調(diào)節(jié)基因表達(dá)的最新研究進(jìn)展,以期為此領(lǐng)域的研究者提供參考。
[Abstract]:Since its inception, CRISPR-Cas9 system has been widely used in genome editing technology, until people inactivated the Ruv C and HNH splicing domains of Cas9 by point mutation to obtain d Cas9. The system has a new function-regulating the level of gene expression. By altering the structure of g-RNA and pairing with RNA-binding proteins, the researchers recruited transcription factors to regulate the transcriptional level of the gene to influence gene expression. In this paper, we mainly discuss the structure and mechanism of CRISPR-d Cas9 system, as well as the latest research progress in regulating gene expression, so as to provide references for researchers in this field.
【作者單位】： 上海市控江中學(xué);同濟(jì)大學(xué)生命科學(xué)與技術(shù)學(xué)院;
【基金】：國家自然科學(xué)青年基金(81402399)項(xiàng)目資助
【分類號(hào)】：Q78
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本文編號(hào)：2470644