醋甘遂與炙甘草免煎顆粒配伍對(duì)CYP2E1、CYP3A4基因和蛋白表達(dá)的影響
發(fā)布時(shí)間：2019-04-30 12:42
【摘要】：目的:通過檢測(cè)大鼠肝功能指標(biāo)和肝組織CYP2E1、CYP3A4的基因、蛋白表達(dá),初步觀察醋甘遂與炙甘草配伍對(duì)肝臟的損傷以及對(duì)肝藥酶2個(gè)亞型的誘導(dǎo)作用。方法:將大鼠按體質(zhì)量隨機(jī)分為正常組、炙甘草組(免煎顆粒劑)、醋甘遂組(研末)、炙甘草-醋甘遂組、甘遂半夏湯組。其中正常組給予生理鹽水灌胃,其余各組給予相應(yīng)藥液灌胃,連續(xù)給藥14天。實(shí)驗(yàn)結(jié)束時(shí),腹主動(dòng)脈取血分離血清和血漿檢測(cè)谷草轉(zhuǎn)氨酶(AST)、谷丙轉(zhuǎn)氨酶(ALT)、乳酸脫氫酶(LDH)含量;取肝組織一部分用福爾馬林固定觀察肝組織病理形態(tài),一部分液氮凍存,采用RT-PCR和western-blot技術(shù)檢測(cè)肝組織中CYP2E1、CYP3A4的基因、蛋白表達(dá)情況。結(jié)果:與正常組相比,各給藥組ALT含量各組變化不明顯,無統(tǒng)計(jì)學(xué)差異(P0.05),AST含量甘遂組以及醋甘遂-炙甘草配伍組、甘遂半夏湯組較正常組降低,其中甘遂半夏湯組降低顯著,有統(tǒng)計(jì)學(xué)差異(P0.05),LDH含量各給藥組均較正常組顯著降低,有統(tǒng)計(jì)學(xué)差異(P0.05,P0.01)。與空白組比較,炙甘草組、醋甘遂組、醋甘遂-炙甘草組和甘遂半夏湯組CYP2E1基金和蛋白表達(dá)均有所升高,CYP3A4的基因和蛋白表達(dá)則有所下降,二者均具有統(tǒng)計(jì)學(xué)差異(P0.01),其中以醋甘遂-炙甘草組升高或降低最為明顯;與醋甘遂-炙甘草組比較,甘遂半夏湯組下調(diào)CYP2E1基金和蛋白表達(dá),上調(diào)CYP3A4的基因和蛋白表達(dá)。結(jié)論:本實(shí)驗(yàn)從分子機(jī)制上證實(shí)了醋甘遂和炙甘草配伍有增毒效應(yīng),以復(fù)方的形式使用則能降低毒性,但在生化指標(biāo)方面未表現(xiàn)出明顯增毒作用,可能還存在其他配伍機(jī)制有待進(jìn)一步研究,且需要配合病理狀態(tài)的研究以確定是否有增效作用。
[Abstract]:Aim: to detect the liver function index and the expression of CYP2E1,CYP3A4 gene and protein in liver tissue of rats, and to observe the liver damage induced by the combination of Radix Glycyrrhizae Sinensis and the induction of two subtypes of hepatic drug enzyme. Methods: rats were randomly divided into three groups according to their body weight: normal group, broiled glycyrrhizin group, vinegar-Gansui group and Gansui Banxia decoction group. The normal group was given saline intragastric administration, the other groups were given the corresponding drug solution, and the other groups were administered continuously for 14 days. At the end of the experiment, the serum and plasma were collected from abdominal aorta to detect the content of (AST), (ALT), LDH (LDH). Part of the liver tissue was fixed with formalin to observe the pathological morphology of liver tissue, and part of the liver tissue was frozen with liquid nitrogen. RT-PCR and western-blot techniques were used to detect the expression of CYP2E1,CYP3A4 gene and protein in the liver tissue. Results: compared with the normal group, the content of ALT in each group was not significantly changed, and there was no statistical difference (P0.05), AST content in Gansui group and the combination group of vinegar and Gansui-fried licorice, and the content of Gansui Banxia decoction group was lower than that in normal group. Gansui Banxia decoction group decreased significantly (P0.05), LDH content was significantly lower than the normal group, there was statistical difference (P0.05, P0.01). Compared with the blank group, the expression of CYP2E1 fund and protein in the grilled licorice group, vinegar Gansui-fried licorice group and Gansui Pinellia decoction group were increased, while the expression of CYP3A4 gene and protein were decreased, the difference was statistically significant (P0.01). Among them, the increase or decrease was the most obvious in the vinegar kansui-broiled glycyrrhizae group. Compared with the vinegar gansui-fried licorice group, Gansui Banxia decoction group down-regulated the expression of CYP2E1 fund and protein, and up-regulated the expression of CYP3A4 gene and protein. Conclusion: the experiment confirmed that the combination of vinegar and licorice could increase toxicity in molecular mechanism, but it could reduce toxicity in the form of compound prescription, but there was no obvious toxic effect in biochemical index, but there was no obvious toxic effect in biochemical index, but there was no obvious toxic effect in combination with Fructus Glycyrrhizae. There may be other compatibility mechanisms to be further studied, and it is necessary to cooperate with the pathological study to determine whether there is synergistic effect.
【作者單位】： 河北中醫(yī)學(xué)院;
【基金】：國家自然科學(xué)基金青年基金項(xiàng)目(81503268) 河北省中醫(yī)藥管理局項(xiàng)目(2015001) 河北省大學(xué)生創(chuàng)新創(chuàng)業(yè)訓(xùn)練計(jì)劃項(xiàng)目(201414432022)
【分類號(hào)】：R285.5
本文編號(hào)：2468769
[Abstract]:Aim: to detect the liver function index and the expression of CYP2E1,CYP3A4 gene and protein in liver tissue of rats, and to observe the liver damage induced by the combination of Radix Glycyrrhizae Sinensis and the induction of two subtypes of hepatic drug enzyme. Methods: rats were randomly divided into three groups according to their body weight: normal group, broiled glycyrrhizin group, vinegar-Gansui group and Gansui Banxia decoction group. The normal group was given saline intragastric administration, the other groups were given the corresponding drug solution, and the other groups were administered continuously for 14 days. At the end of the experiment, the serum and plasma were collected from abdominal aorta to detect the content of (AST), (ALT), LDH (LDH). Part of the liver tissue was fixed with formalin to observe the pathological morphology of liver tissue, and part of the liver tissue was frozen with liquid nitrogen. RT-PCR and western-blot techniques were used to detect the expression of CYP2E1,CYP3A4 gene and protein in the liver tissue. Results: compared with the normal group, the content of ALT in each group was not significantly changed, and there was no statistical difference (P0.05), AST content in Gansui group and the combination group of vinegar and Gansui-fried licorice, and the content of Gansui Banxia decoction group was lower than that in normal group. Gansui Banxia decoction group decreased significantly (P0.05), LDH content was significantly lower than the normal group, there was statistical difference (P0.05, P0.01). Compared with the blank group, the expression of CYP2E1 fund and protein in the grilled licorice group, vinegar Gansui-fried licorice group and Gansui Pinellia decoction group were increased, while the expression of CYP3A4 gene and protein were decreased, the difference was statistically significant (P0.01). Among them, the increase or decrease was the most obvious in the vinegar kansui-broiled glycyrrhizae group. Compared with the vinegar gansui-fried licorice group, Gansui Banxia decoction group down-regulated the expression of CYP2E1 fund and protein, and up-regulated the expression of CYP3A4 gene and protein. Conclusion: the experiment confirmed that the combination of vinegar and licorice could increase toxicity in molecular mechanism, but it could reduce toxicity in the form of compound prescription, but there was no obvious toxic effect in biochemical index, but there was no obvious toxic effect in biochemical index, but there was no obvious toxic effect in combination with Fructus Glycyrrhizae. There may be other compatibility mechanisms to be further studied, and it is necessary to cooperate with the pathological study to determine whether there is synergistic effect.
【作者單位】： 河北中醫(yī)學(xué)院;
【基金】：國家自然科學(xué)基金青年基金項(xiàng)目(81503268) 河北省中醫(yī)藥管理局項(xiàng)目(2015001) 河北省大學(xué)生創(chuàng)新創(chuàng)業(yè)訓(xùn)練計(jì)劃項(xiàng)目(201414432022)
【分類號(hào)】：R285.5
【相似文獻(xiàn)】
相關(guān)期刊論文 前3條
1 ;醋甘遂粉治療肝硬化腹水15例[J];廣西中醫(yī)藥;1997年04期
2 陳明校，孫洪慶，劉向選，沈?qū)毺铮叽浩�，祖景蘭;單味醋甘遂粉治療慢性腎炎水腫1例[J];中西醫(yī)結(jié)合實(shí)用臨床急救;1995年04期
3 ;[J];;年期
相關(guān)會(huì)議論文 前1條
1 束曉云;丁安偉;張麗;黃海燕;;甘遂和醋甘遂醇提物及其不同極性部位的藥效和毒性研究[A];中華中醫(yī)藥學(xué)會(huì)四大懷藥與地道藥材研究論壇暨中藥炮制分會(huì)第二屆第五次學(xué)術(shù)會(huì)與第三屆會(huì)員代表大會(huì)論文集[C];2007年
，本文編號(hào)：2468769
本文鏈接：http://sikaile.net/kejilunwen/jiyingongcheng/2468769.html
最近更新
教材專著
