【摘要】：為了建立一種快速、特異、靈敏的檢測I群禽腺病毒的液相基因芯片方法。根據(jù)Hexon基因序列設計特異引物,上游引物5'端加TAG序列,下游引物5'端加生物素,進行PCR擴增,將擴增產(chǎn)物與鏈霉素親和蛋白、磁珠37℃孵育30 min,通過Luminex200儀器分析。用所建立的液相基因芯片方法進行特異性、靈敏度、重復性及病料樣品的檢測。結果該方法的特異性強;檢測的敏感性可達1×102copies/μL;批內批間的變異系數(shù)都在5%以下;檢測結果與SYBR Green I熒光PCR方法 100%相符。表明建立的液相基因芯片方法特異性好、靈敏高、重復性好,可用于I群禽腺病毒的檢測。
[Abstract]:In order to establish a rapid, specific and sensitive liquid-phase microarray method for detection of group I avian adenoviruses. According to the Hexon gene sequence, specific primers were designed. The upstream primer 5 'end added TAG sequence and downstream primer 5' end added biotin. The amplified product was amplified by PCR. The amplified product was incubated with streptomycin affinity protein at 37 鈩，

本文編號：2464016