菜豆普通細(xì)菌性疫病抗性基因定位
[Abstract]:Common bacterial blight of kidney bean is one of the most serious diseases in the world. Long kidney bean No. 5 is the main cultivar of Heilongjiang Province in China, and it has good resistance to common bacterial blight of kidney bean. In order to locate the resistance gene derived from Longrendou No. 5, a F2 segregation population containing 785 individual plants was constructed in this study. Based on the population, a genetic map consisting of 206 SSR markers with a total length of 1648.42 cm and an average genetic distance of 8.00 cm between markers was constructed. The map contains 12 linkage groups, the average length of each linkage group is 137.37 cm, and the number of markers on linkage group is 3 / 35. Combined with the results of greenhouse phenotypic identification, the complete region mapping method of QTL Ici Mapping v4.0 software was used for QTL localization and effect estimation. A disease-resistant QTL. was detected on the Pv06 chromosome 14 days after inoculation. The locus was located between marker p6s249 and p6s183, and the additive effect value was 0.44, indicating that the synergist gene originated from Longrendou 5, the LOD value was 5.93, the phenotypic contribution rate was 4.61%, and the effective value of the disease resistance QTL was relatively low. It will play a role in the cultivation of stable and lasting varieties resistant to common bacterial blight of kidney bean. Finally, 11 pairs of SSR primers closely linked to the resistance gene to common bacterial blight of kidney bean showed that the SSR marker p6s249 was highly significantly associated with the resistance to common bacterial blight of kidney bean (P0.001). This marker can be used for disease resistance molecular breeding.
【作者單位】: 中國(guó)農(nóng)業(yè)科學(xué)院作物科學(xué)研究所;
【基金】:國(guó)家現(xiàn)代農(nóng)業(yè)產(chǎn)業(yè)技術(shù)體系建設(shè)專項(xiàng)(CARS-09) 國(guó)家科技支撐計(jì)劃項(xiàng)目(2013BAD01B03-18a) 中國(guó)農(nóng)業(yè)科學(xué)院科技創(chuàng)新工程項(xiàng)目資助~~
【分類號(hào)】:S436.43
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