牛源MRSA新疆流行株的分離鑒定及spa基因多態(tài)性分型
發(fā)布時(shí)間：2019-04-03 09:02
【摘要】：【目的】明確新疆地區(qū)牛源耐甲氧西林金黃色葡萄球菌(MRSA)流行株的流行特點(diǎn),為有效防控新疆奶牛乳房炎和子宮內(nèi)膜炎提供參考依據(jù)�！痉椒ā坎捎梅蛛x培養(yǎng)、生化鑒定、藥敏試驗(yàn)等方法對(duì)牛源MRSA進(jìn)行鑒定,并以PCR擴(kuò)增牛源MRSA新疆流行株spa基因的X區(qū),測(cè)序結(jié)果列提交至spa分型數(shù)據(jù)庫(kù)(http://www.ridom.de/spaserver/)進(jìn)行spa基因多態(tài)性分型�！窘Y(jié)果】從221份樣品中共檢測(cè)出71株金黃色葡萄球菌,其中對(duì)苯唑西林抑菌直徑小于10mm的有8株;而以mec A引物進(jìn)行PCR擴(kuò)增,發(fā)現(xiàn)擴(kuò)增結(jié)果呈陽(yáng)性的有13株,MRSA檢出率為18.3%。spa基因分型有4種,分別為t779、t2883、t13751和t1939,其中5株為t779(r08),4株為t2883(r07-r23-r21-r17-r34),2株為t13751(r07-r23-r12-r21-r17-r12),2株為t1939(r07-r23-r02-r34),即t779(r08)為MRSA新疆流行株的主要spa基因分型�！窘Y(jié)論】MRSA在新疆不同地區(qū)均有分布,且其耐藥情況不斷變化,以t779(r08)為MRSA新疆流行株的主要spa基因分型。
[Abstract]:[objective] to clarify the epidemic characteristics of methicillin-resistant Staphylococcus aureus (MRSA) strain in Xinjiang, and to provide reference for the prevention and control of mastitis and endometritis of dairy cows in Xinjiang. [methods] isolation culture and biochemical identification were used. MRSA of bovine origin was identified by drug sensitivity test, and X region of spa gene of bovine MRSA strain was amplified by PCR. The sequencing results were submitted to the spa typing database (http://www.ridom.de/spaserver/) for spa gene polymorphism typing. [results] 71 strains of Staphylococcus aureus were detected from 221 samples. Among them, 8 strains of oxacillin had a diameter smaller than that of 10mm. The results of PCR amplification with mec A primers showed that 13 strains were positive, and the positive rate of MRSA was 4 kinds of 18.3%.spa genotyping, which were t779, t2883, t13751 and t1939 respectively, among which 5 were t779 (R08), 5 of them were t779 (R08), and 5 of them were t779 (R08). 4 were t2883 (r07-r23-r21-r17-r34), 2 were t13751 (r07-r23-r12-r21-r17-r12), 2 were t1939 (r07-r23-r02-r34), 2 were t13751 (r07-r23-r12-r21-r17-r12), 2 were t1939 (r07-r23-r02-r34). [conclusion] T779 (R08) is the main spa genotyping of MRSA strains in Xinjiang [conclusion] MRSA is distributed in different areas of Xinjiang, and its drug resistance is constantly changing, and t779 (R08) is the main spa genotyping of Xinjiang epidemic strains of MRSA. [conclusion] T779 (R08) is the main genotyping of spa in Xinjiang endemic strains of MRSA.
【作者單位】： 石河子大學(xué)動(dòng)物科技學(xué)院;烏魯木齊市動(dòng)物疾病控制與診斷中心;中國(guó)農(nóng)業(yè)科學(xué)院蘭州獸醫(yī)研究所;
【基金】：國(guó)家“十三五”重點(diǎn)研發(fā)計(jì)劃項(xiàng)目(2016YFD0500900) 新疆研究生科研創(chuàng)新項(xiàng)目(XJGRI2016040) 兵團(tuán)中青年科技創(chuàng)新領(lǐng)軍人才計(jì)劃項(xiàng)目(2016BC001) 重慶市社會(huì)民生科技創(chuàng)新專項(xiàng)項(xiàng)目(cstc2016shmszx80121)
【分類號(hào)】：S852.611
本文編號(hào)：2453090
[Abstract]:[objective] to clarify the epidemic characteristics of methicillin-resistant Staphylococcus aureus (MRSA) strain in Xinjiang, and to provide reference for the prevention and control of mastitis and endometritis of dairy cows in Xinjiang. [methods] isolation culture and biochemical identification were used. MRSA of bovine origin was identified by drug sensitivity test, and X region of spa gene of bovine MRSA strain was amplified by PCR. The sequencing results were submitted to the spa typing database (http://www.ridom.de/spaserver/) for spa gene polymorphism typing. [results] 71 strains of Staphylococcus aureus were detected from 221 samples. Among them, 8 strains of oxacillin had a diameter smaller than that of 10mm. The results of PCR amplification with mec A primers showed that 13 strains were positive, and the positive rate of MRSA was 4 kinds of 18.3%.spa genotyping, which were t779, t2883, t13751 and t1939 respectively, among which 5 were t779 (R08), 5 of them were t779 (R08), and 5 of them were t779 (R08). 4 were t2883 (r07-r23-r21-r17-r34), 2 were t13751 (r07-r23-r12-r21-r17-r12), 2 were t1939 (r07-r23-r02-r34), 2 were t13751 (r07-r23-r12-r21-r17-r12), 2 were t1939 (r07-r23-r02-r34). [conclusion] T779 (R08) is the main spa genotyping of MRSA strains in Xinjiang [conclusion] MRSA is distributed in different areas of Xinjiang, and its drug resistance is constantly changing, and t779 (R08) is the main spa genotyping of Xinjiang epidemic strains of MRSA. [conclusion] T779 (R08) is the main genotyping of spa in Xinjiang endemic strains of MRSA.
【作者單位】： 石河子大學(xué)動(dòng)物科技學(xué)院;烏魯木齊市動(dòng)物疾病控制與診斷中心;中國(guó)農(nóng)業(yè)科學(xué)院蘭州獸醫(yī)研究所;
【基金】：國(guó)家“十三五”重點(diǎn)研發(fā)計(jì)劃項(xiàng)目(2016YFD0500900) 新疆研究生科研創(chuàng)新項(xiàng)目(XJGRI2016040) 兵團(tuán)中青年科技創(chuàng)新領(lǐng)軍人才計(jì)劃項(xiàng)目(2016BC001) 重慶市社會(huì)民生科技創(chuàng)新專項(xiàng)項(xiàng)目(cstc2016shmszx80121)
【分類號(hào)】：S852.611
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