調(diào)控CD163基因表達(dá)的miRNA鑒定及其在PRRSV感染中的作用分析
發(fā)布時(shí)間：2019-04-02 21:00
【摘要】：豬繁殖與呼吸綜合征(PRRS)是由豬繁殖與呼吸綜合征病毒(PRRSV)感染引起的一種危害大、控制難的豬傳染性疾病。PRRSV必須通過特異性受體誘導(dǎo)才能入侵宿主細(xì)胞,而CD163就是其中一個(gè)關(guān)鍵受體。在獲得靶向作用CD163基因miRNA的基礎(chǔ)上,進(jìn)一步研究發(fā)現(xiàn)過表達(dá)miR-181c、miR-4262可極顯著抑制細(xì)胞內(nèi)CD163 mRNA的表達(dá)(P0.01);且在Marc-145細(xì)胞中證實(shí)miR-181c、miR-23b、miR-4262均具有極顯著抑制PRRSV增殖的功能(P0.01),其中miR-4262抑制效果最佳。3個(gè)miRNA抗PRRSV增殖的分子機(jī)制比較復(fù)雜,miR-181c、miR-4262可通過靶向抑制受體CD163發(fā)揮抗PRRSV增殖作用,而miR-23b是通過其他途徑發(fā)揮作用。并首次發(fā)現(xiàn)miR-4262具有抑制PRRSV增殖的作用,但其具體的分子作用機(jī)制還有待進(jìn)一步深入研究。
[Abstract]:Porcine reproductive and respiratory syndrome (PRRS) is a porcine infectious disease caused by porcine reproductive and respiratory syndrome virus (PRRSV) infection. PRRSv must be induced by specific receptors in order to invade host cells. CD163 is one of the key receptors. On the basis of targeting CD163 gene miRNA, it was found that over-expression of miR-181c,miR-4262 could significantly inhibit the expression of CD163 mRNA in cells (P0.01). Moreover, in Marc-145 cells, it was confirmed that miR-181c,miR-23b,miR-4262 could inhibit the proliferation of PRRSV significantly (P0.01), among which miR-4262 had the best inhibitory effect. The molecular mechanism of three miRNA against PRRSV proliferation was complex, and miR-181c, was found to be the most effective in inhibiting the proliferation of PRRSV (P0.01). MiR-4262 can inhibit the proliferation of PRRSV by targeting receptor CD163, while miR-23b plays a role through other pathways. It is found for the first time that miR-4262 can inhibit the proliferation of PRRSV, but its molecular mechanism needs to be further studied.
【作者單位】： 動(dòng)物胚胎工程及分子育種湖北省重點(diǎn)實(shí)驗(yàn)室/湖北省農(nóng)業(yè)科學(xué)院畜牧獸醫(yī)研究所;
【基金】：國家自然科學(xué)基金(編號(hào):31501924) 湖北省自然科學(xué)基金(編號(hào):2015CFA105) 湖北省技術(shù)創(chuàng)新重大項(xiàng)目(編號(hào):2016ABA117) 湖北省科技支撐計(jì)劃(編號(hào):2014BBB010) 湖北省農(nóng)業(yè)科技創(chuàng)新中心創(chuàng)新團(tuán)隊(duì)項(xiàng)目(編號(hào):2016-620-000-001-022) 湖北省農(nóng)業(yè)科學(xué)院競爭性計(jì)劃項(xiàng)目(編號(hào):2016jzxjh029)
【分類號(hào)】：S852.651
本文編號(hào)：2452917
[Abstract]:Porcine reproductive and respiratory syndrome (PRRS) is a porcine infectious disease caused by porcine reproductive and respiratory syndrome virus (PRRSV) infection. PRRSv must be induced by specific receptors in order to invade host cells. CD163 is one of the key receptors. On the basis of targeting CD163 gene miRNA, it was found that over-expression of miR-181c,miR-4262 could significantly inhibit the expression of CD163 mRNA in cells (P0.01). Moreover, in Marc-145 cells, it was confirmed that miR-181c,miR-23b,miR-4262 could inhibit the proliferation of PRRSV significantly (P0.01), among which miR-4262 had the best inhibitory effect. The molecular mechanism of three miRNA against PRRSV proliferation was complex, and miR-181c, was found to be the most effective in inhibiting the proliferation of PRRSV (P0.01). MiR-4262 can inhibit the proliferation of PRRSV by targeting receptor CD163, while miR-23b plays a role through other pathways. It is found for the first time that miR-4262 can inhibit the proliferation of PRRSV, but its molecular mechanism needs to be further studied.
【作者單位】： 動(dòng)物胚胎工程及分子育種湖北省重點(diǎn)實(shí)驗(yàn)室/湖北省農(nóng)業(yè)科學(xué)院畜牧獸醫(yī)研究所;
【基金】：國家自然科學(xué)基金(編號(hào):31501924) 湖北省自然科學(xué)基金(編號(hào):2015CFA105) 湖北省技術(shù)創(chuàng)新重大項(xiàng)目(編號(hào):2016ABA117) 湖北省科技支撐計(jì)劃(編號(hào):2014BBB010) 湖北省農(nóng)業(yè)科技創(chuàng)新中心創(chuàng)新團(tuán)隊(duì)項(xiàng)目(編號(hào):2016-620-000-001-022) 湖北省農(nóng)業(yè)科學(xué)院競爭性計(jì)劃項(xiàng)目(編號(hào):2016jzxjh029)
【分類號(hào)】：S852.651
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1 吳俊靜;彭先文;喬木;武華玉;劉貴生;梅書棋;;利用雙熒光素酶報(bào)告系統(tǒng)鑒定靶向作用豬CD163基因的miRNA[J];湖北農(nóng)業(yè)科學(xué);2015年19期
【共引文獻(xiàn)】
相關(guān)期刊論文 前1條
1 吳俊靜;喬木;武華玉;彭先文;梅書棋;;調(diào)控CD163基因表達(dá)的miRNA鑒定及其在PRRSV感染中的作用分析[J];江蘇農(nóng)業(yè)科學(xué);2017年13期
，本文編號(hào)：2452917
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