【摘要】：[目的]本文旨在對(duì)薔薇科果樹(shù)液泡膜單糖轉(zhuǎn)運(yùn)蛋白(TMT)基因家族成員進(jìn)行鑒定,分析其染色體定位、序列特性、進(jìn)化關(guān)系及在白梨中的表達(dá)特征,為薔薇科果樹(shù)TMT基因功能研究和利用奠定基礎(chǔ)。[方法]基于蘋(píng)果、草莓、梅、桃、白梨、西洋梨和黑樹(shù)莓7個(gè)薔薇科果樹(shù)物種的全基因組測(cè)序結(jié)果,應(yīng)用生物信息學(xué)方法進(jìn)行序列分析,通過(guò)RT-q PCR技術(shù)分析白梨TMT基因在各器官組織中和果實(shí)不同發(fā)育階段的表達(dá)特性。[結(jié)果]蘋(píng)果、草莓、梅、桃、白梨、西洋梨和黑樹(shù)莓等TMT基因家族成員數(shù)量分別為5、2、4、4、6、4、2,各物種TMT基因不均勻地分布在2~4條染色體上。TMT蛋白結(jié)構(gòu)高度保守,均存在11個(gè)α螺旋跨膜結(jié)構(gòu)域,氨基酸序列長(zhǎng)度700左右,等電點(diǎn)(p I)為4.79~5.93,最大疏水系數(shù)為0.312~0.438,為疏水性蛋白,亞細(xì)胞定位預(yù)測(cè)均位于液泡膜。系統(tǒng)進(jìn)化分析表明,7個(gè)薔薇科果樹(shù)物種的TMT家族基因明顯聚為兩組,組Ⅰ中的TMT基因可能為其主要的功能基因。TMT基因家族經(jīng)歷過(guò)2次全基因組復(fù)制事件,進(jìn)化過(guò)程中主要受純化選擇作用。定量分析發(fā)現(xiàn),白梨中鑒定的Pb TMT1、Pb TMT2、Pb TMT3在成熟葉中和果實(shí)膨大期表達(dá)量最高,Pb TMT4的表達(dá)量隨著果實(shí)發(fā)育不斷增高,Pb TMT6的表達(dá)量在成熟葉中最高,而在果實(shí)發(fā)育后期急劇下降。[結(jié)論]薔薇科果樹(shù)TMT基因家族成員高度保守,主要在液泡膜上起重要功能,不僅在果實(shí)糖分積累過(guò)程中發(fā)揮重要作用,還參與了植物發(fā)育和抗逆過(guò)程。
[Abstract]:[objective] to identify the family members of vacuolar monosaccharide transporter (TMT) gene in fruit trees of Rosaceae, and to analyze its chromosome localization, sequence characteristics, evolutionary relationship and expression characteristics in white pear. It lays a foundation for the study and utilization of TMT gene in Rosaceae fruit trees. [methods] based on the whole genome sequencing results of 7 species of Rosaceae fruit trees, including apple, strawberry, plum, peach, white pear, western pear and black raspberry, the sequence analysis was carried out by bioinformatics method. RT-q PCR technique was used to analyze the expression characteristics of TMT gene in different organs and tissues and different stages of fruit development. [results] the number of TMT gene family members in apple, strawberry, plum, peach, white pear, western pear and black raspberry was 5, 2, 4, 4, 6, 4, 2, respectively. The TMT gene of each species was unevenly distributed on 2 and 4 chromosomes, and the structure of TMT protein was highly conserved. There are 11 偽-helix transmembrane domains, the amino acid sequence length is about 700, the isoelectric point (p I) is 4.79 ~ 5.93, the maximum hydrophobic coefficient is 0.312 ~ 0.438, and the subcellular localization prediction is located in the vacuolar membrane. Phylogenetic analysis showed that the TMT family genes of 7 species of Rosaceae fruit trees were obviously clustered into two groups, and the TMT gene in group 鈪，

本文編號(hào)：2443965