【摘要】：發(fā)掘和利用抗旱基因資源是水稻抗旱育種的基礎(chǔ)。本研究利用PVC管鑒定法,對24份轉(zhuǎn)入抗旱主效基因的材料進行苗期抗旱特性鑒定,設(shè)干旱脅迫(DD)和非干旱脅迫(WW)兩處理,研究中采用了最大根長、根干物質(zhì)重、葉干物質(zhì)重、根葉長比、分蘗數(shù)、葉面積、水份消耗量、水份利用率作為鑒定指標(biāo)。研究結(jié)果顯示,對于導(dǎo)入抗旱主效基因的材料,經(jīng)苗期抗旱性鑒定都具有一定的抗旱性,但抗旱的程度有差異。脅迫下,所有材料的最大根長值超過正常生長下的值,處理間差異顯著;在根葉長比、分蘗數(shù)、水份消耗量、水份利用率上部分材料表現(xiàn)為正增長;在根干物質(zhì)重、葉干物質(zhì)重、葉面積上均表現(xiàn)為負(fù)增長,處理間差異顯著,只是個別材料的值與WW下的生長量差異不顯著。鑒定出5份抗旱性表現(xiàn)較好的材料,5份中除IR83376-B-B-71-1外,都轉(zhuǎn)入了兩個以上的抗旱基因,說明聚合抗旱基因?qū)μ岣呖购敌允怯行У摹ｈb定指標(biāo)間存在較好的相關(guān)性并考慮指標(biāo)值測定的方便準(zhǔn)確,最大根長、根葉長比、分蘗數(shù)、水份消耗量可作為苗期較好的抗旱性鑒定指標(biāo)。
[Abstract]:It is the foundation of rice drought resistance breeding to explore and utilize drought resistance gene resources. In this study, the drought resistance characteristics of 24 transgenic materials with major drought resistance genes were identified by PVC method. Two treatments, drought stress (DD) and non drought stress (WW), were used in this study. The maximum root length and root dry matter weight were used in the study. Leaf dry matter weight, root / leaf length ratio, tillering number, leaf area, water consumption and water utilization ratio were used as identification indexes. The results showed that the drought resistance of the materials introduced with major drought resistance genes at seedling stage had certain drought resistance, but the degree of drought resistance was different. Under stress, the maximum root length value of all materials was higher than that under normal growth, and there were significant differences among the treatments, and some of the materials showed positive growth in root / leaf length ratio, tillering number, water consumption and water utilization rate. The root dry matter weight and leaf area showed negative growth, the difference among treatments was significant, but there was no significant difference between the value of individual materials and the growth under WW. Five materials with good drought resistance were identified, and more than two drought resistance genes were transferred into 5 of them except IR83376-B-71-1, indicating that the polymerized drought resistance gene is effective for improving drought resistance. There was a good correlation among the identification indexes and the determination of the index value was convenient and accurate, the maximum root length, root / leaf length ratio, tillering number and water consumption could be used as the identification indexes for drought resistance at seedling stage.
【作者單位】： 瀾滄縣富邦鄉(xiāng)農(nóng)業(yè)服務(wù)中心;云南省農(nóng)業(yè)科學(xué)院糧食作物研究所;
【基金】：云南省科技計劃項目(2015ZA003) 國家863計劃項目(2014AA10A603;2011AA10Z104)共同資助
【分類號】：S511

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本文編號：2443305