【摘要】：為探討共培養(yǎng)條件下沙門菌對(duì)糞腸球菌N41分離株生長(zhǎng)特性及其不同生長(zhǎng)時(shí)期26種毒力基因轉(zhuǎn)錄的影響,將N41菌株、沙門菌菌株單獨(dú)/混合培養(yǎng)后,分別進(jìn)行菌落計(jì)數(shù)并繪制生長(zhǎng)曲線,觀察其生長(zhǎng)狀況及影響,同時(shí)提取其對(duì)數(shù)中期、對(duì)數(shù)后期、穩(wěn)定前期和穩(wěn)定期的總RNA,SYBR GreenⅠ實(shí)時(shí)熒光定量PCR方法分析N41菌株26種毒力基因的轉(zhuǎn)錄情況。結(jié)果顯示,共培養(yǎng)后沙門菌和N41生長(zhǎng)均受到影響,其中,在N41菌體濃度降低約3.9倍,而沙門菌菌體濃度降低約110倍。在N41菌株26種毒力基因中,沙門菌促進(jìn)了ebpA、ebpC、rnjB、ace、ebpR、psr等12種毒力基因在4個(gè)不同生長(zhǎng)時(shí)期的轉(zhuǎn)錄,但同時(shí)也降低了毒力基因SlyA和sprE在4個(gè)生長(zhǎng)期中轉(zhuǎn)錄水平;另外,除了CylL-S、CylL-L、efaA和AS在4個(gè)生長(zhǎng)時(shí)期變化不明顯外,其余的大部分毒力基因都在對(duì)數(shù)后期轉(zhuǎn)錄水平有明顯上升,但在其他生長(zhǎng)時(shí)期則表現(xiàn)不同。這說明,兩菌共培養(yǎng)后,N41菌株明顯抑制了沙門菌的生長(zhǎng),同時(shí)沙門菌也整體促進(jìn)了N41菌株毒力基因的轉(zhuǎn)錄。本試驗(yàn)為研究細(xì)菌間相互作用機(jī)制以及腸球菌的致病機(jī)制奠定基礎(chǔ)。
[Abstract]:In order to investigate the effects of Salmonella on the growth characteristics of Enterococcus faecalis N41 isolates and the transcription of 26 virulent genes at different growth stages under co-culture conditions, the strains N41 and Salmonella strains were isolated or co-cultured. Colony count was carried out and growth curve was drawn to observe the growth status and influence. At the same time, the total RNA, of logarithmic, Prophase and stable phase were extracted. The transcription of 26 virulent genes of N41 strain was analyzed by SYBR Green 鈪，

本文編號(hào)：2433612